A5034600040- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
University of California, Santa Barbara
2014-2021
ABSTRACT Contact-dependent growth inhibition (CDI) systems encode CdiA effectors, which bind to specific receptors on neighboring bacteria and deliver C-terminal toxin domains suppress target cell growth. Two classes of effectors that distinct surface have been identified, but the molecular basis receptor specificity is not understood. Alignment BamA-specific EC93 from Escherichia coli OmpC-specific EC536 E. 536 suggests receptor-binding domain resides within a central region varies between...
Contact-dependent growth inhibition (CDI) is a mode of bacterial competition orchestrated by the CdiB/CdiA family two-partner secretion proteins. The CdiA effector extends from surface CDI(+) inhibitor cells, binds to receptors on neighbouring bacteria and delivers toxin domain derived its C-terminal region (CdiA-CT). Here, we show that CdiA-CT translocation requires proton-motive force (pmf) within target bacteria. pmf also critical for colicin toxins, which exploit energized Ton Tol...
Bacteria use several different secretion systems to deliver toxic EndoU ribonucleases into neighboring cells. Here, we present the first structure of a prokaryotic toxin in complex with its cognate immunity protein. The contact-dependent growth inhibition CdiA-CTSTECO31 from Escherichia coli STEC_O31 adopts eukaryotic fold and shares greatest structural homology nuclease domain coronavirus Nsp15. contains canonical His-His-Lys catalytic triad same arrangement as domains, but lacks...
Contact-dependent growth inhibition (CDI) systems are widespread amongst Gram-negative bacteria where they play important roles in inter-cellular competition and biofilm formation. CDI+ use cell-surface CdiA proteins to bind neighboring deliver C-terminal toxin domains. cells also express CdiI immunity that specifically neutralize toxins delivered from adjacent siblings. Genomic analyses indicate cdi loci commonly found on plasmids genomic islands, suggesting these Type 5 secretion spread...
Contact-dependent growth inhibition (CDI) is a common form of interbacterial competition in which cells use CdiA effectors to deliver toxic proteins into their neighbors. recognizes target bacteria through specific receptor molecules on the cell surface.