A5026980545- Cellular transport and secretion
- Tuberous Sclerosis Complex Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Heme Oxygenase-1 and Carbon Monoxide
- Hereditary Neurological Disorders
- Retinal Development and Disorders
- Amyotrophic Lateral Sclerosis Research
- Neuroscience and Neural Engineering
- Ubiquitin and proteasome pathways
- Developmental Biology and Gene Regulation
- Neural dynamics and brain function
- Intracerebral and Subarachnoid Hemorrhage Research
- Lipid Membrane Structure and Behavior
- Lysosomal Storage Disorders Research
- S100 Proteins and Annexins
- Neurosurgical Procedures and Complications
- Neurogenesis and neuroplasticity mechanisms
- Genetic and Kidney Cyst Diseases
University of Cambridge
2024
Vrije Universiteit Amsterdam
2017-2019
Amsterdam Neuroscience
2017-2019
Uppsala University Hospital
2014
Uppsala University
2013
The complicated secondary molecular and cellular mechanisms following traumatic brain injury (TBI) are still not fully understood. In the present study, we have used mass spectrometry to identify specific proteins in an vitro model of TBI. A standardized was induced by scalpel cuts through a mixed cell culture astrocytes, oligodendrocytes neurons. Twenty-four hours after injury, medium whole-cell fractions were collected for analysis. We found 53 46 fraction that specifically expressed known...
Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies human brain disorders. However we lack networks with balanced excitatory-inhibitory activities, which are suitable for single cell analysis. We generated low-density hPSC-derived GABAergic and glutamatergic cortical neurons. used two different co-culture models astrocytes. show that these have synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging mRNA These...
The neural tube consists of progenitors (NPs) that acquire different characteristics during gestation due to patterning factors. However, the influence such factors on human pluripotent stem cells (hPSCs) in vitro differentiation is often unclear. This study compared induction protocols involving with single SMAD inhibition (SSI), retinoic acid (RA) administration and dual (DSI). While derived NP expressed known markers, they differed their expression profile potential. Cortical neuronal...
Neurons communicate by regulated secretion of chemical signals from synaptic vesicles (SVs) and dense-core (DCVs). Here, we investigated the maturation these two secretory pathways in micro-networks human iPSC-derived neurons. These abundantly expressed endogenous SV DCV markers, including neuropeptides. transport was microtubule dependent, preferentially anterograde axons, 2-fold faster axons than dendrites. were strictly Ca2+ SNARE dependent. capacity matured until day vitro (DIV) 36, with...
Mutation of the ATL1 gene is one most common causes hereditary spastic paraplegia (HSP), a group genetic neurodegenerative conditions characterised by distal axonal degeneration corticospinal tract axons. Atlastin-1, protein encoded ATL1, three mammalian atlastins, which are homologous dynamin-like GTPases that control endoplasmic reticulum (ER) morphology fusing tubules to form three-way junctions characterise ER networks. However, it not clear whether atlastin-1 required for correct in...
Traumatic brain injury (TBI) is a heterogeneous disease, and the discovery of diagnostic prognostic TBI biomarkers highly desirable in order to individualize patient care. We have previously published study which we identified possible by mass spectrometry 24 h after cell culture model. Ezrin-radixin-moesin (ERM) proteins were found abundantly medium trauma, present extracellular ezrin as biomarker for trauma analyzing from injured primary neurons glia measuring cerebrospinal fluid (CSF)...
Crosstalk between neurons and oligodendrocytes is important for proper brain functioning. Multiple co-culture methods have been developed to study oligodendrocyte maturation, myelination or the effect of on neurons. However, most these contain cells derived from animal models. In current protocol, we human with oligodendrocytes. Neurons precursor (OPCs) were differentiated separately pluripotent stem according previously published protocols. To neuron-glia cross-talk, OPCs plated in mode...
Abstract Mutation of the ATL1 gene is one most common causes hereditary spastic paraplegia (HSP), a group genetic neurodegenerative conditions characterised by distal axonal degeneration corticospinal tract axons. Atlastin-1, protein encoded , three mammalian atlastins, which are homologous dynamin-like GTPases that control endoplasmic reticulum (ER) morphology fusing tubules to form three-way junctions characterise ER networks. However, it not clear whether atlastin-1 required for correct...
Abstract Fission of transport tubules from early endosomes is required for endosomal sorting, but mechanisms tubule fission (ETF) are incompletely understood. We show protrudin acts at ER-endosome contacts to promote ETF and endosome-to-Golgi traffic. Protrudin-mediated its ability interact with ER-localised VAP proteins, phosphoinositides KIF5. These properties also regulated the distance between tubules. The defective phenotype increased tubulation in cells lacking was phenocopied by...