- Phytase and its Applications
- Crop Yield and Soil Fertility
- Genetics and Plant Breeding
- Plant Micronutrient Interactions and Effects
- Genetic Mapping and Diversity in Plants and Animals
- Food composition and properties
- Genetically Modified Organisms Research
- Phytochemicals and Antioxidant Activities
- Wheat and Barley Genetics and Pathology
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Insect Resistance and Genetics
- Transgenic Plants and Applications
- Biofuel production and bioconversion
- Plant Reproductive Biology
- Animal Genetics and Reproduction
- Iron Metabolism and Disorders
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Metalloenzymes and iron-sulfur proteins
- Chromosomal and Genetic Variations
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Genetic and phenotypic traits in livestock
- Plant Molecular Biology Research
Agricultural Research Service
2016-2025
Texas A&M University
2024
Iowa State University
2009-2020
Antalya Eğitim ve Araştırma Hastanesi
2020
United States Department of Agriculture
1991-2016
Ames National Laboratory
2013
Arizona State University
2003
University of Nebraska–Lincoln
1995-1998
Purdue University West Lafayette
1991-1998
University of Copenhagen
1995
Genomic selection (GS) is a method to estimate the breeding values of individuals by using markers throughout genome. We evaluated accuracies GS data from five traits on 446 oat (Avena sativa L.) lines genotyped with 1005 Diversity Array Technology (DArT) and two methods (ridge regression–best linear unbiased prediction [RR-BLUP] BayesCπ) under various training designs. Our objectives were (i) determine accuracy increasing marker density population size, (ii) assess when divided over time,...
Significance Each species contains a subset of genes that are uniquely present in species; the functions and origins vast majority these “orphan genes” not well-understood. Expression Arabidopsis QQS ( Qua-Quine Starch ; At3g30720 ) orphan transgene increases level protein soybean lines with high low acts across flowering plants to increase content maize rice. Our results begin dissect mechanism by identifying it binds conserved transcription factor nuclear Y, subunit C4 (NF-YC4). Increased...
The assembly of 11S globulin seed storage proteins in plants is regulated part by the activity a protease that cleaves between asparagine and glycine residues. Post-translational cleavage subunit precursors into acidic basic polypeptides associated with ability subunits trimers to aggregate hexamers vitro . An present extracts from immature soybean seeds specifically Vicia faba mature proteins. Sequence microanalysis has been used demonstrate proglycinin prolegumin are cut at legitimate site...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTComparative digestibility of legume storage proteinsS. Suzanne Nielsen, Sudhir S. Deshpande, Mark A. Hermodson, and M. Paul ScottCite this: J. Agric. Food Chem. 1988, 36, 5, 896–902Publication Date (Print):September 1, 1988Publication History Published online1 May 2002Published inissue 1 September 1988https://pubs.acs.org/doi/10.1021/jf00083a004https://doi.org/10.1021/jf00083a004research-articleACS PublicationsRequest reuse permissionsArticle...
ABSTRACT β‐glucan, a soluble fiber found in oat ( Avena sativa L.) grain, is good for human health, and selection higher levels of this compound regarded as an important breeding objective. Recent advances DNA markers present opportunity to investigate new methods polygenic traits such β‐glucan concentration. Our objectives study were compare genomic, marker‐assisted, best linear unbiased prediction (BLUP)–based phenotypic short‐term response ability maintain genetic variance Starting with...
11S seed storage proteins are synthesized as precursors that cleaved post-translationally in vacuoles by an asparaginyl endopeptidase. To study the specificity of reaction catalyzed this endopeptidase, we prepared a series octapeptides and mutant legumin B G4 glycinin subunits. These contained amino acid mutations region surrounding cleavage site. The endopeptidase had absolute for Asn on N-terminal side severed peptide bond but exhibited little acids C-terminal side. ability unmodified...
The B subunit of Escherichia coli heat labile enterotoxin (LT-B) is a potent oral immunogen with potential for use as vaccine, carrier molecule to deliver antigens gut-associated lymphoid tissues, and possibly an adjuvant make coadministered vaccines more effective. LT-B produced in plants was shown be functional immunogenic animals humans. In this work, we show that maize-derived strongly associated starch granules endosperm. Using immunogold labeling/electron microscopy, cell...
Objectives of this study were to understand how opaque-2 (o2) mutation and quality protein maize (QPM) affect kernel composition starch structure, property, enzyme digestibility. Kernels o2 contained less (9.6-12.5%) than those the wild-type (WT) counterparts (12.7-13.3%). a severe mutant B46o2 also (66.9%) B46wt (73.0%). QPM starches amylose (28.0 26.0%, respectively) others (31.9-33.7%). The consisted amylopectin with fewest branch chains DP 13-24. Thus, was most susceptible porcine...
Maize opaque2 (o2) mutations are beneficial for endosperm nutritional quality but cause negative pleiotropic effects reasons that not fully understood. Direct targets of the bZIP transcriptional regulator encoded by o2 include pdk1 and pdk2 specify pyruvate phosphate dikinase (PPDK). This enzyme reversibly converts AMP, pyrophosphate, phosphoenolpyruvate to ATP, orthophosphate, provides diverse functions in plants. study addressed PPDK function maize starchy where it is highly abundant...
Abstract The Ga2 locus controls unilateral cross incompatibility in Zea species. Molecular characterization of this indicates that fully functional loci contain two components function male and female reproductive tissues, Ga2P Ga2F respectively. Some mays varieties have been reported to be capable overcoming the barrier including widely used inbred line Mo17. Our objective was better understand structure, distribution To do this, we first examined ability overcome across a diverse sample...
ABSTRACT Phytate is the dominant storage form of phosphorus (P) in mature cereal and oil grains. Phosphorus bound phytate nutritionally unavailable to monogastric animals thus contributes water pollution because it excreted waste. Also, can chelate certain minerals exacerbate human mineral deficiencies. Our primary objective was develop a rapid inexpensive method measuring inorganic P (P i ) concentrations maize ( Zea mays L.). The procedure reported herein derived from previously published...
The ga1 locus of maize confers unilateral cross incompatibility, preventing pollination between females carrying the incompatible Ga1-s allele and males not a corresponding compatible allele. To characterize this system at molecular level, we carried out transcript profiling experiment in which silks from near isogenic lines alleles were compared. While several differentially expressed genes identified, only one mapped to known location ga1. This gene is pectin methylesterase (PME),...
The maize (Zea mays) aleurone layer occupies the single outermost of endosperm. defective kernel1 (dek1) gene is a central regulator required for cell fate specification. dek1 mutants have pleiotropic phenotypes including lack cells, aborted embryos, carotenoid deficiency, and soft, floury endosperm deficient in zeins. Here we describe thick aleurone1 (thk1) mutant that defines novel negative function regulation differentiation. Mutants possess multiple layers cells as well embryos. Clonal...