A5005866571- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Immunodeficiency and Autoimmune Disorders
- HIV Research and Treatment
- Immune Response and Inflammation
- CAR-T cell therapy research
- Tuberculosis Research and Epidemiology
- Influenza Virus Research Studies
- Chronic Lymphocytic Leukemia Research
- Diabetes and associated disorders
- Immune responses and vaccinations
- Cell Adhesion Molecules Research
- Respiratory viral infections research
- Mycobacterium research and diagnosis
- Cytomegalovirus and herpesvirus research
- Lymphoma Diagnosis and Treatment
- T-cell and Retrovirus Studies
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Cancer Immunotherapy and Biomarkers
- Galectins and Cancer Biology
- Histiocytic Disorders and Treatments
Imperial College London
2005-2025
Breast Center
2018
Invitae (United States)
2018
Dallas Surgical Group
2018
Advocate Health Care
2018
University of Oxford
2007-2017
Medawar Building for Pathogen Research
2009-2017
Lung Institute
2017
Cancer Research UK
1994-2015
Jenner Institute
2002-2011
A group of mAb recognizing the 200- and/or 220-kDa determinants (CD45R) leukocyte common Ag such as 2H4, WR16, MD4.3, and SN130 cross-block each other showing that they recognize a closely related epitope. The antibody UCHL1 reacts with 180-kDa determinant exhibits reciprocal T subset distribution pattern to CD45R group. Peripheral blood cells were 40% positive for 58 65% antibodies; less than 1% stained both. On activation CD45R+,UCHL1- by PHA, up became both day 3. By 7, fell from 90 21%...
The properties of human lymphocyte fractions isolated either by sheep red cell(E) rosetting or fluorescence-activated cell sorting after staining with UCHT1 monoclonal anti-T antibody have been compared. Two populations E+ cells very different phenotype and function identified. E+/UCHT1+ respond well to the T mitogens phytohemagglutinin concanavalin A provide help for an in vitro specific response. They can also suppress response allegeneic peripheral blood mononuclear cells. In contrast,...
While memory T cells are maintained by continuous turnover, it is not clear how human regulatory CD4+CD45RO+CD25hi Foxp3+ lymphocyte populations persist throughout life. We therefore used deuterium labeling of cycling in vivo to determine whether these could be replenished proliferation. found that CD4+CD45RO+Foxp3+CD25hi lymphocytes were highly proliferative, with a doubling time 8 days, compared CD4+CD45RO+Foxp3–CD25– (24 days) or naive CD4+CD45RA+Foxp3–CD25– (199 days). However, the...
The CD4 (or T4) surface antigen of human T lymphocytes is an important part the receptor for immunodeficiency virus (HIV). After binding to receptor, HIV may enter cell and induce formation syncytia. In attempt identify site more closely, monoclonal antibodies (Mab's) were tested their ability block infection in a syncytium assay, epitopes so identified mapped by antibody cross-blocking. that showed strong inhibition fell into two main families while third group Mab's blocked syncytia weakly...
Summary Human natural killer (NK) cells form a circulating population in state of dynamic homeostasis. We investigated NK cell homeostasis by labelling dividing vivo using deuterium‐enriched glucose young and elderly healthy subjects patients with viral infection. Following 24‐hr intravenous infusion 6,6‐D 2 ‐glucose, CD3 – CD16 + sorted from peripheral blood mononuclear (PBMC) fluorescence‐activated sorter (FACS) were analysed for DNA deuterium content gas chromatography mass spectrometry...
Abstract Boosting bacillus Calmette-Guérin (BCG)-primed mice with a recombinant adenovirus expressing Mycobacterium tuberculosis Ag 85A by different administration routes has very effects on protection against aerosol challenge M. tuberculosis. Mice boosted intradermally make strong splenic CD4 and CD8 Th1 cytokine responses to 85A, but show no change in lung mycobacterial burden over BCG primed animals. In contrast, intranasally greatly reduced much weaker response an increased purified...
Background. A 23-valent unconjugated pneumococcal polysaccharide vaccine (23vP), routinely administered at the age of 65, has limited effectiveness, and revaccination induces attenuated antibody responses. It is not known whether polysaccharide-protein conjugated vaccines (PCV), although highly effective in infants, offer any immunological advantages over 23vP adults.
Abstract Using limiting dilution analysis, we investigated proliferative responses of UCHL1 + and − T cell populations to compare the precursor frequencies following recall alloantigen stimulation, complexity cellular interactions within populations. We find high antigen among UCHL l , but not cells. In contrast, both contain similar responsive Our results are consistent with single‐hit kinetics in as well show no complex responding conclusion, difference between response 1 cells observed...
UCHL1 is a murine monoclonal antibody that recognises 180-185 kD determinant on CD4 (72%) and CD8 (36%) positive T cells. This effective in formalin fixed paraffin embedded tissues, using the immunoperoxidase method. One hundred forty three cases of malignant lymphoma were examined. Neoplastic cells 100% Mycosis fungoides (n = 10), 83% peripheral cell 25), 78% (T-ALL) acute lymphoblastic 9) stained by this antibody. In addition, staining was seen histiocytosis intestine 13), condition now...
This paper describes the use of a panel seven monoclonal antibodies (selected so as to include reagents reactive with both epithelial and lymphoid cells) for distinguishing between anaplastic carcinoma high grade lymphoma. Details are given immunohistological reactions these against wide range normal malignant tissues number practical instances in which antibody enabled diagnosis be made when routine histological examination had been inconclusive.
Human immunodeficiency virus (HIV) binds to cells via an interaction between CD4 and the envelope glycoprotein, gp120. Previous studies have localized high affinity binding site for gp120 first domain of CD4, monoclonal antibodies (mAbs) reactive with this region compete thereby block infectivity syncytium formation. Despite a detailed understanding little is known subsequent events leading membrane fusion entry. We describe two new mAbs third that inhibit steps critical HIV cell fusion....
Memory T cells can be divided into central-memory (T(CM)) and effector-memory (T(EM)) cells, which differ in their functional properties. Although both subpopulations persist long term, it is not known whether they are maintained by similar mechanisms. We used vivo labeling with deuterated glucose to measure the turnover of CD4(+) healthy humans. The CD45R0(+)CCR7(-) T(EM) subpopulation was shown have a rapid proliferation rate 4.7% per day compared 1.5% for CD45R0(+)CCR7(+) T(CM) cells;...
Abstract Two monoclonal antibodies, 1F5 and B1, directed against the CD20 (Bp35) antigen were found to have both stimulatory inhibitory effects on B cells. 1F5, but not induces small resting tonsillar cells prolymphocytic leukemia enlarge, rapidly increase their RNA synthesis, become responsive growth factors present in mixed lymphocyte reaction supernatants. In addition, a moderate thymidine uptake, which is accompanied by enhanced viability of cells, any total cell number or detectable...
The HER2 protooncogene encodes a 185-kD transmembrane phosphoglycoproteins, human epidermal growth factor receptor 2 (p185HER2), whose amplified expression on the cell surface can lead to malignant transformation. Overexpression of HER2/p185HER2 is strongly correlated with progression ovarian and breast carcinomas. Recent studies have shown that T cells be targeted bispecific antibody react against tumor in vitro. We developed F(ab')2 molecule consisting humanized arm specificity p185HER2...