A5012196373- Developmental Biology and Gene Regulation
- Marine Ecology and Invasive Species
- Animal Genetics and Reproduction
- Genetic diversity and population structure
- Neurobiology and Insect Physiology Research
- MicroRNA in disease regulation
- Genetics and Physical Performance
- Congenital heart defects research
- Protist diversity and phylogeny
- Reproductive biology and impacts on aquatic species
- Marine Biology and Environmental Chemistry
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Neuroendocrine regulation and behavior
- Mass Spectrometry Techniques and Applications
- Retinal Development and Disorders
Kansas State University
2020-2022
Konan University
2010-2021
Abstract Tunicate larvae have a non-reproductive gonadotropin-releasing hormone (GnRH) system with multiple ligands and receptor heterodimerization enabling complex regulation. In Ciona intestinalis type A larvae, one of the gnrh genes, gnrh2 , is conspicuously expressed in motor ganglion nerve cord, which are homologous structures to hindbrain spinal respectively, vertebrates. The gene also proto-placodal sensory neurons, proposed homologue vertebrate olfactory neurons. occupy dispersal...
The notochord is a defining feature of the chordates. transcription factor Brachyury (Bra) key regulator fate but here we show that it not unitary master in model chordate
The tunicate Ciona intestinalis , an invertebrate chordate, has recently emerged as a powerful model organism for gene regulation analysis. However, few studies have been conducted to identify and characterize its transcription start sites (TSSs) promoters at the genome-wide level. Here, using TSS-seq, we identified TSSs scale characterized in C. . Specifically, TSS clusters (TSCs), high-density regions of TSS-seq tags, each which appears originate from identical promoter. TSCs were found...
In vertebrate embryos, dorsal midline tissues, including the notochord, prechordal plate, and floor play important roles in patterning of central nervous system, somites, endodermal tissues by producing extracellular signaling molecules, such as Sonic hedgehog (Shh). Ciona , hedgehog.b one two genes, is expressed plate embryonic neural tube, while none genes are notochord. We have identified a cis -regulatory region that was sufficient to drive reporter gene expression plate. The also drove...
The relatively simple structure of ascidians and the number associated molecular resources that are available make an excellent experimental system for Investigating mechanisms underlying neural tube formation. ascidian demonstrates same basic morphology as vertebrates. We have described expression tubespecific gene CiNut1, which is expressed within precursor cells from gastrula stage, along entire length during its In this study, we focused on transcriptional regulate CiNut1 expression....
Abstract The notochord is a defining feature of the chordates. transcription factor Brachyury (Bra) key regulator fate but here we show that it not unitary master in model chordate Ciona . Ectopic Bra expression only partially reprograms other cell types to notochord-like transcriptional profile and subset notochord-enriched genes are unaffected by CRISPR disruption. We identify Foxa.a Mnx as potential co-regulators find combinatorial cocktails more effective at reprograming than alone....
Summary An expression system for transgenes is an important tool gene function analyses. The present study describes a promoter sequence that can drive ubiquitous in ascidian development and optimization of plasmid vector maximizing transgene expression. Using the elongation factor 1-alpha as test promoter, we evaluated vector, polyadenylation signals, cleavage ribosome entry site sequences. In embryos, most element efficient was signal. signal derived from SV40 3′-UTR C. intestinalis actin...
A major goal in biology is to understand the rules by which cis-regulatory sequences control spatially and temporally precise expression patterns. Here we present a systematic dissection of proximal enhancer for notochord-specific transcription factor brachyury ascidian chordate Ciona. The study uses quantitative image-based reporter assay that incorporates dual-reporter strategy variable electroporation efficiency. We identified mutated multiple predicted binding sites interest based on...
ABSTRACT Tunicate larvae have a non-reproductive gonadotropin-releasing hormone (GnRH) system with multiple ligands and receptor heterodimerization enabling complex regulation. In the Ciona larva, one of gnrh genes, gnrh2 , is conspicuously expressed in motor ganglion nerve cord, which are homologous structures to hindbrain spinal respectively, vertebrates. The gene also proto-placodal sensory neurons, proposed homologue vertebrate olfactory neurons. tunicate occupy dispersal stage, yet role...