A5086318639- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Wnt/β-catenin signaling in development and cancer
- Virology and Viral Diseases
- Mosquito-borne diseases and control
- Nerve injury and regeneration
- Chronic Lymphocytic Leukemia Research
- Transgenic Plants and Applications
- Cancer-related gene regulation
- Food composition and properties
- Blood groups and transfusion
- Rabies epidemiology and control
- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Genetic factors in colorectal cancer
- Crystallization and Solubility Studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Venomous Animal Envenomation and Studies
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- Proteins in Food Systems
- Signaling Pathways in Disease
- Axon Guidance and Neuronal Signaling
- Nutritional Studies and Diet
Lesaffre (France)
2024
LFB (France)
2015-2021
LFB (United States)
2014
Centre National de la Recherche Scientifique
2011
Centre de Physiopathologie de Toulouse-Purpan
2005-2011
Université de Toulouse
2011
Université Toulouse III - Paul Sabatier
2008-2011
Inserm
2003-2011
Marie Curie
2010
Fondation de l'Avenir
2006
The long serum t1/2 of IgGs is ensured by their interaction with the neonatal Fc receptor (FcRn), which salvages IgG from intracellular degradation. glycosylation thought not to influence FcRn binding and longevity in vivo. In this article, we demonstrate that hypersialylation asparagine 297 (N297) enhances persistence. This polarized achieved using a novel mutation, glutamate residue deletion at position 294 (Del) endows an up 9-fold increase lifespan. strongest impact was observed when Del...
Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase, initially discovered as part of the NPM-ALK fusion protein, resulting from t(2;5) translocation that frequently associated with anaplastic large-cell lymphomas. The native ALK protein normally expressed in developing and, at weaker level, adult nervous system. We recently demonstrated oncogenic, constitutively kinase-activated was antiapoptotic when Jurkat lymphoblastic cells treated cytotoxic drugs. In contrast, we now show...
Following infection of the central nervous system (CNS), immune is faced with challenge eliminating pathogen without causing significant damage to neurons, which have limited capacities renewal. In particular, it was thought that neurons were protected from direct attack by cytotoxic T lymphocytes (CTL) because they do not express major histocompatibility class I (MHC I) molecules, at least steady state. To date, most our current knowledge on specifics neuron-CTL interaction based studies...
Despite the reasonably long half-life of immunoglogulin G (IgGs), market pressure for higher patient convenience while conserving efficacy continues to drive IgG improvement. is dependent on neonatal Fc receptor (FcRn), which among other functions, protects from catabolism. FcRn binds domain at an acidic pH ensuring that endocytosed will not be degraded in lysosomal compartments and then released into bloodstream. Consistent with this mechanism action, several Fc-engineered increased...
While glyco-engineered monoclonal antibodies (mAbs) with improved antibody-dependent cell-mediated cytotoxicity (ADCC) are reaching the market, extensive efforts have also been made to improve their pharmacokinetic properties generate biologically superior molecules. Most therapeutic mAbs human or humanized IgG molecules whose half-life is dependent on neonatal Fc receptor FcRn. FcRn reduces catabolism by binding domain of endocytosed in acidic lysosomal compartments, allowing them be...
Infection by Borna disease virus (BDV) enables the study of molecular mechanisms whereby a can persist in central nervous system and lead to altered brain function absence overt cytolysis inflammation. This neurotropic infects wide variety vertebrates causes behavioral diseases. The basis BDV-induced impairment remains largely unknown. Here, we investigated whether BDV infection neurons affected synaptic activity, studying rate vesicle (SV) recycling, good indicator activity. Vesicular...
Blockade of the human epidermal growth factor receptor 3 (HER3) and downstream phosphatidylinositide 3-kinase (PI3K)/AKT pathway is a prerequisite for overcoming drug resistance to develop novel treatments cancers that are not eligible currently approved targeted therapies. To this end, we generated specific antibodies (Abs) against domain 1 (D1) (D3) HER3 recognize epitopes do overlap with neuregulin-binding site. The fully H4B-121 Ab mouse monoclonal Abs 16D3-C1 9F7-F11 inhibited tumor in...
N-cadherin is expressed throughout skeletal myogenesis and has been proposed to be involved in the differentiation program of myogenic precursors. Here, we further characterize involvement its mechanism action at onset differentiation, through controlled activation by plating isolated C2 myoblasts on surfaces coated with a chimeric Ncad-Fc homophilic ligand (N-cadherin ectodomain fused immunoglobulin G Fc fragment). We show that substitutes for cell density promoting myogenin troponin T...
The physiological role of the prion protein is largely unknown. Here, clustering at surface GT1‐7 cells was observed upon anti‐prion antibody treatments. This associated with a rapid and transient phosphorylation mitogen activated kinases (MAPKs) extracellular receptor 1 2 (ERK1/2), also microtubule‐destabilizing stathmin serine 16. specificity this antibody‐mediated activation ascertained by its inhibition small interfering RNA. ERK1/2 but not that abolished MAPK/ERK kinase inhibitor U0126,...
The monoclonal antibody (mAb) ST40, specific for the immunoglobulin complementarity-determining region (CDR) 3-like loop in domain 1 of CD4 molecule, inhibits human immunodeficiency virus type (HIV-1) promoter activity and viral transcription HIV-infected cells. To design synthetic peptides from ST40 paratope that could mimic these biological properties, a set 220 overlapping 12-mer frameshifted by one residue, corresponding to deduced amino acid sequence, was synthesized Spot method tested...
ABSTRACT The neurotropic virus Borna disease (BDV) persists in the central nervous systems of a wide variety vertebrates and causes behavioral disorders. BDV represents an intriguing example whose persistence neurons leads to altered brain function absence overt cytolysis inflammation. bases BDV-induced impairment remain largely unknown. To better characterize neuronal response infection, we compared proteomes primary cultures cortical with without infection. We used two-dimensional liquid...
Bread can vary in textural and nutritional attributes based on differences the bread making process (e.g., flour type, fermentation agent, time). Four recipes (BRs) made with sourdough preferments (BR1, white flour; BR2, whole grain flour) or regular yeast breads (BR3, BR4, were evaluated for texture, digestibility, their effect metabolic activity composition of gut microbiota using texture profile analysis (TPA) coupled vitro upper gastrointestinal (GIT) digestion colonic (Colon-on-a-plate™...
Novel molecules that directly target the neonatal Fc receptor (FcRn) and/or gamma receptors (FcγRs) are emerging as promising treatments for immunoglobulin G (IgG)-dependent autoimmune pathologies. Mutated regions and monoclonal antibodies FcRn currently in clinical development hold promise reducing levels of circulating IgG. Additionally, engineered structures containing multimeric allow dual targeting FcγRs; however, their tolerance needs to first be validated phase I studies. Here, time,...
The normal cellular prion protein is a small sialoglycoprotein highly expressed in neurons, the physiological function of which largely unknown. Due to extensive N-glycosylations with wide range oligosaccharides, displays complex glycosylation pattern that could be relevance for its function. patterns adult mouse and rat brain, neuronal cell lines, appeared heterogeneous, as distinct levels glycoforms were revealed by immunoblotting corresponding samples. Amongst N2a neuroblastoma cells low...
Monoclonal antibodies are now widely used to measure the concentration of steroid hormones in human serum samples. The great development molecular engineering techniques over past 10 years has made possible improvement specificity and/or sensitivity selected antibodies. We have obtained two monoclonal antibodies, 17E12E5 and 10G6D6, using estradiol-6-ethyl methoxy carbonyl (EMC)-bovine albumin (BSA) as immunogen. To tentatively improve their affinities for natural estradiol, we initiated...