A5019649839- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Neurogenesis and neuroplasticity mechanisms
- Retinal Diseases and Treatments
- Genetic Neurodegenerative Diseases
- Ocular Surface and Contact Lens
- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- Mitochondrial Function and Pathology
- Microplastics and Plastic Pollution
- Retinopathy of Prematurity Studies
- Genetics and Neurodevelopmental Disorders
- Heavy Metal Exposure and Toxicity
- Polydiacetylene-based materials and applications
- Trypanosoma species research and implications
- Corneal Surgery and Treatments
- RNA Interference and Gene Delivery
- Ocular Disorders and Treatments
- RNA Research and Splicing
- Neurogenetic and Muscular Disorders Research
- CRISPR and Genetic Engineering
- Phagocytosis and Immune Regulation
- Lysosomal Storage Disorders Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Fish Biology and Ecology Studies
Universidade de Santiago de Compostela
2018-2024
Université Paris Cité
2024
Inserm
2024
Instituto de Investigación Biomédica de A Coruña
2022
Abstract Cellular senescence is a stress response that limits the proliferation of damaged cells by establishing permanent cell cycle arrest. Different stimuli can trigger but excessive production or impaired clearance these lead to their accumulation during aging with deleterious effects. Despite this potential negative side senescence, its physiological role as pro‐regenerative and morphogenetic force has emerged recently after identification programmed embryogenesis wound healing limb...
Due to their extensive use, the release of zinc oxide nanoparticles (ZnO NP) into environment is increasing and may lead unintended risk both human health ecosystems. Access ZnO NP brain has been demonstrated, so potential toxicity on nervous system a matter particular concern. Although evaluation reported in several previous studies, specific effects are not completely understood and, particularly, genetic material organism behaviour poorly addressed. We evaluated toxic vitro vivo, role ions (Zn
The aim of this work was to develop niosomes for the ocular delivery epalrestat, a drug that inhibits polyol pathway and protects diabetic eyes from damage linked sorbitol production accumulation. Cationic were made using polysorbate 60, cholesterol, 1,2-di-O-octadecenyl-3-trimethylammonium propane. characterized dynamic light scattering, zeta-potential, transmission electron microscopy determine their size (80 nm; polydispersity index 0.3 0.5), charge (−23 +40 mV), shape (spherical)....
Abstract The pretectum is a complex region of the caudal diencephalon which in adult zebrafish comprises both retinorecipient (parvocellular superficial, central, intercalated, paracommissural, and periventricular) non‐retinorecipient (magnocellular posterior, accessory) pretectal nuclei distributed from periventricular to superficial regions. We conducted comprehensive study connections by using neuronal tracing with fluorescent carbocyanine dyes. This reveals specialization efferent...
Ocular health may strongly benefit from the supply of antioxidant agents that counteract free radicals and reactive oxygen species responsible for long-term eye diseases. Additionally, natural antioxidants like resveratrol can inhibit bacteria growth restore microbiota. However, their use is hindered by limited solubility, fast degradation, low ocular permeability. This work aimed to overcome these limitations preparing single mixed micelles Pluronic® F127 casein serve as nanocarriers....
Cellular senescence is considered a stress response imposing stable cell cycle arrest to restrict the growth of damaged cells. More recently however, cellular was identified during mouse embryo development at particular structures specific periods time. This programmed has been proposed serve developmental and morphogenetic functions potentially represent an evolutionary origin senescence. also described take place bird (chick quail) amphibian (xenopus axoltl) development. Fish have show...
NOP56 belongs to a C/D box small nucleolar ribonucleoprotein complex that is in charge of cleavage and modification precursor ribosomal RNAs assembly the 60S subunit. An intronic expansion gene causes Spinocerebellar Ataxia type 36, typical late-onset autosomal dominant ataxia. Although vertebrate animal models were created for expansion, none was studied loss function NOP56. We zebrafish loss-of-function model nop56 which shows 70% homology with human gene. observed severe neurodegenerative...
It is largely assumed that the teleost retina shows continuous and active proliferative neurogenic activity throughout life. However, when delving into literature, one finds assumptions about a highly proliferation in adult are based on studies which was not quantified comparative way at different life stages or mainly studied juveniles/young adults. Here, we performed systematic study of constitutive from early developing (2 days post-fertilisation) to aged (up 3-4 years zebrafish. The...
Niemann Pick disease type C (NPC) is an autosomal recessive neurodegenerative lysosomal disorder characterized by accumulation of lipids in different organs. Clinical manifestations can start at any age and include hepatosplenomegaly, intellectual impairment, cerebellar ataxia. NPC1 the most common causal gene, with over 460 mutations heterogeneous pathological consequences. We generated a zebrafish model CRISPR/Cas9 carrying homozygous mutation exon 22, which encodes end cysteine-rich...
Abstract The study of neurogenesis is essential to understanding fundamental developmental processes and for the development cell replacement therapies central nervous system disorders. Here, we designed an in vivo drug screening protocol developing zebrafish find new molecules signalling pathways regulating ventral spinal cord. This unbiased screen revealed that 4 cyclooxygenase (COX) inhibitors reduced generation serotonergic interneurons These results fitted very nicely with available...
Gene therapy is a promising therapeutic approach that has experienced significant groth in recent decades, with gene nanomedicines reaching the clinics. However, it still necessary to continue developing novel vectors able carry, protect, and release nucleic acids into target cells, respond widespread demand for new therapies address current unmet clinical needs. We propose here use of zebrafish embryos as an vivo platform evaluate potential newly developed nanosystems applications cancer...
Abstract It is largely assumed that the teleost retina shows continuous and active proliferative neurogenic activity throughout life. But when deepening in literature one finds assumptions about a highly proliferation adult are based on studies which was not quantified comparative way at different life stages or mainly studied juveniles/young adults. Here, we performed systematic study of constitutive from early developing (2 days post-fertilization) to aged (up 3-4 years zebrafish. Mitotic...
Abstract Diabetic retinopathy (DR) is one of the leading causes blindness in world. While there a major focus on study juvenile/adult DR, effects hyperglycemia during early retinal development are less well studied. Recent works embryonic zebrafish models nutritional revealed that leads to decreased cell numbers mature types, which has been related modest increase apoptotic death and altered differentiation (Singh et al. 2019; Titialii-Torres Morris 2022). However, how impacts proliferation...