A5008716859- Cardiac Ischemia and Reperfusion
- Extracellular vesicles in disease
- Cardiac Arrest and Resuscitation
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Anesthesia and Neurotoxicity Research
- ATP Synthase and ATPases Research
- MicroRNA in disease regulation
- Cardiovascular Disease and Adiposity
- Cardiovascular Function and Risk Factors
- Cardiac electrophysiology and arrhythmias
- Thermal Regulation in Medicine
- Genetic and Kidney Cyst Diseases
- Cardiac Fibrosis and Remodeling
- Chemotherapy-induced cardiotoxicity and mitigation
- Respiratory viral infections research
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Receptor Mechanisms and Signaling
- Influenza Virus Research Studies
- Renin-Angiotensin System Studies
- Phagocytosis and Immune Regulation
- Atherosclerosis and Cardiovascular Diseases
- Nitric Oxide and Endothelin Effects
- Ferroptosis and cancer prognosis
University of Chile
2016-2025
Center for Climate and Resilience Research
2023-2025
Advanced Center for Chronic Diseases
2015-2024
University College London
2017-2020
Cardiovascular Institute of the South
2017
University of Louisville
2010
Mitochondria are key organelles for ATP production in cardiomyocytes, which is regulated by processes of fission and fusion. We hypothesized that the mitochondria fusion protein dynamin-related 1 (Drp1) inhibition, attenuates ischemia-reperfusion (I/R) injury through modifications mitochondrial metabolism. Rats were subjected to I/R coronary artery ligation, isolated cardiomyocytes treated with an ischemia-mimicking solution. In vivo, cardiac function, myocardial infarction area, morphology...
Abstract Many patients with ischaemic heart disease also have diabetes. As myocardial infarction is a major cause of mortality and morbidity in these patients, treatments that increase cell survival response to ischaemia reperfusion are needed. Exosomes—nano‐sized, lipid vesicles released from cells—can protect the hearts non‐diabetic rats. We previously showed exosomal HSP70 activates cardioprotective signalling pathway cardiomyocytes culminating ERK1/2 HSP27 phosphorylation. Here, we...
Extracellular vesicles (EVs) such as exosomes are nano-sized that carry proteins and miRNAs can transmit signals between cells. We hypothesized from endothelial cells protective to cardiomyocytes. Co-culture of primary adult rat cardiomyocytes with normoxic HUVEC separated by a cell-impermeable membrane reduced the percentage cardiomyocyte death following simulated ischaemia reperfusion (sIR) 80 ± 11% 51 4% (P < 0.05; N = 5). When EVs were removed HUVEC-conditioned medium it was no longer...
Community-acquired pneumonia (CAP) severity scores can identify patients at low risk for mortality who may be suitable ambulatory care. Here, we follow the clinical course of hospitalized with CAP due to 2009 H1N1 influenza.To evaluate role as predictors mortality.This was a secondary data analysis influenza confirmed by reverse transcriptase polymerase chain reaction enrolled in CAPO (Community-Acquired Pneumonia Organization) international cohort study. PSI (Pneumonia Severity Index),...
Ischemic preconditioning (IPC) limits myocardial infarct size through the activation of PI3K–Akt signal cascade; however, little is known about roles individual PI3K isoforms in cardioprotection. We aimed, therefore, to elucidate role PI3Kα isoform cardioprotection Pharmacological inhibition was assessed isolated-perfused mouse hearts subjected ischemia/reperfusion injury (IRI), either during IPC procedure or at reperfusion. abrogated IPC-induced protective effect reperfusion, but not when...
Abstract Aims Genetic and pharmacological inhibition of mitochondrial fission induced by acute myocardial ischaemia/reperfusion injury (IRI) has been shown to reduce infarct size. The clinically used anti-hypertensive heart failure medication, hydralazine, is known have anti-oxidant anti-apoptotic effects. Here, we investigated whether hydralazine confers cardioprotection inhibiting Drp1-mediated fission. Methods results Pre-treatment with was inhibit both membrane depolarisation oxidative...
Abstract Small extracellular vesicles (EVs) are novel players in vascular biology. However, a thorough understanding of their production and function remains elusive. Endothelial senescence is key feature ageing thus, an attractive therapeutic target for the treatment disease. In this study, we sought to characterize EV senescent endothelial cells. To achieve this, Human Umbilical Vascular Cells (HUVECs) were replicated until they reached senescence, as determined by measurement...
Hypertension is a disease associated to increased plasma levels of angiotensin II (Ang II). Ang can regulate proliferation, migration, ROS production and hypertrophy vascular smooth muscle cells (VSMCs). However, the mechanisms by which affect VSMCs remain be fully elucidated. In this context, autophagy, process involved in self-digestion proteins organelles, has been described remodeling. Therefore, we sought investigate if regulates VSMC through an autophagy-dependent mechanism. To test...
ABSTRACT Del Nido cardioplegia (DNC), a blood‐and‐crystalloid solution containing high and low concentrations of potassium calcium, respectively, is used as single‐dose antegrade infusion to induce immediate cardiac arrest in the surgery patients with cardiovascular diseases requiring extracorporeal circulation. Adding cardioprotective molecules may further reduce damage‐triggered ischemia/reperfusion (I/R) injury. Angiotensin‐(1–9) (Ang‐(1–9)) angiotensin‐(1–7) (Ang‐(1–7)), members...
The Reperfusion Injury Salvage Kinase (RISK) pathway is considered the main pro-survival kinase cascade mediating ischaemic preconditioning (IPC) cardioprotective effect. To assess role of PI3K-Akt, its negative regulator PTEN and other proteins such as ERK STAT3 in context IPC, C57BL/6 mouse hearts were retrogradely perfused a Langendorff system subjected to 4 cycles 5 min. ischaemia reperfusion prior 35 global 120 reperfusion. Wortmannin, PI3K inhibitor, was administered either at...
Vascular smooth muscle cells (VSMC) dedifferentiation from a contractile to synthetic phenotype contributes atherosclerosis. Atherosclerotic tissue has chronic inflammatory component with high levels of tumor necrosis factor-α (TNF-α). VSMC atheromatous plaques have increased autophagy, mechanism responsible for protein and intracellular organelle degradation. The aim this study was evaluate whether TNF-α induces switching VSMCs effect depends on autophagy. Rat aortic A7r5 cell line used as...
Ventricular arrhythmias are a common cause of sudden cardiac death, and their occurrence is higher in obese subjects. Abnormal gating ryanodine receptors (RyR2), the calcium release channels sarcoplasmic reticulum, can produce ventricular arrhythmias. Since obesity promotes oxidative stress RyR2 redox-sensitive channels, we investigated whether activity was altered mice. Mice fed high fat diet (HFD) became after eight weeks exhibited significant increase Single isolated from hearts mice were...
The vascular cell adhesion molecule (VCAM-1) is a transmembrane sialoglycoprotein detected in activated endothelial and smooth muscle cells involved the transmigration of inflammatory into damaged tissue. Widely used as pro-inflammatory marker, its potential role targeting has not been thoroughly explored.We discuss current evidence supporting VCAM-1 atherosclerosis, diabetes, hypertension ischemia/reperfusion injury.There emerging that more than biomarker may be promising therapeutic target...
As the pandemic of 2009 H1N1 influenza A virus progressed, more patients required hospitalisation. The objective this study is to describe characteristics and clinical course hospitalised with infection in Chile. This was a prospective, observational 100 consecutive RT-PCR-confirmed A, admitted Puerto Montt General Hospital (Puerto Montt, Chile). Information obtained regarding contact history, demographics, laboratory values course. primary reason for hospitalisation pneumonia, 75% patients....