A5087259102- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Heat shock proteins research
- Adipose Tissue and Metabolism
- Biosensors and Analytical Detection
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Pancreatic function and diabetes
- Adenosine and Purinergic Signaling
- Exercise and Physiological Responses
Centro de Biología Molecular Severo Ochoa
2021-2024
Universidad Autónoma de Madrid
2021-2024
Consejo Superior de Investigaciones Científicas
2021-2024
Centre for Biomedical Network Research on Rare Diseases
2021-2024
Instituto de Salud Carlos III
2021-2024
Research Institute Hospital 12 de Octubre
2021-2024
Centro de Investigación Biomédica en Red
2023
Institut de Biomedicina de la Universitat de Barcelona
2023
Universitat de Barcelona
2023
Instituto de Investigación de Enfermedades Raras
2022
The mitochondrial ATP synthase emerges as key hub of cellular functions controlling the production ATP, signaling, and fate. It is regulated by ATPase inhibitory factor 1 (IF1), which highly abundant in neurons. Herein, we ablated or overexpressed IF1 mouse neurons to show that dose defines fraction active/inactive enzyme vivo, thereby function reactive oxygen species (mtROS). Transcriptomic, proteomic, metabolomic analyses indicate regulates metabolism, synaptic function, cognition....
Abstract ATPase Inhibitory Factor 1 (IF1) regulates the activity of mitochondrial ATP synthase. The expression IF1 in differentiated human and mouse cells is highly variable. In intestinal cells, overexpression protects against colon inflammation. Herein, we have developed a conditional IF1-knockout model epithelium to investigate role function tissue homeostasis. results show that IF1-ablated mice increased synthase/hydrolase activities, leading profound dysfunction pro-inflammatory...
The coexistence of two pools ATP synthase in mitochondria has been largely neglected despite vitro indications for the existence reversible active/inactive state transitions F1-domain enzyme. Herein, using cells and from mouse tissues, we demonstrate vivo synthase: one active, other IF1-bound inactive. IF1 is required oligomerization inactivation proper cristae formation. Immunoelectron microscopy shows co-distribution synthase, placing inactive "sluggish" preferentially at tips....
Abstract Lung cancer is the leading cause of cancer-related death worldwide despite success therapies targeting oncogenic drivers and immune-checkpoint inhibitors. Although metabolic enzymes offer additional targets for therapy, precise proteome lung adenocarcinomas unknown, hampering its clinical translation. Herein, we used Reverse Phase Protein Arrays to quantify changes in glycolysis, oxidation pyruvate, fatty acid metabolism, oxidative phosphorylation, antioxidant response protein...
The ability of alternative splicing mechanisms to control gene expression is increasingly being recognized as relevant for adipose tissue function. SF3B1, a key component the SF3B complex directly involved in spliceosome formation, was previously reported be significantly induced brown under cold-induced thermogenic activation. Here, we identify that noradrenergic cAMP-mediated stimulation increases SF3B1 and beige adipocytes. We further show pladienolide-B, drug binds inhibit pre-mRNA by...
T cells experience metabolic reprogramming to an enhanced glycolysis upon activation. Herein, we have investigated whether ATPase Inhibitory Factor 1 (IF1), the physiological inhibitor of mitochondrial ATP synthase, participates in rewiring a particular phenotype. We show that activation naive CD4