A5086169736- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- PARP inhibition in cancer therapy
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Corneal Surgery and Treatments
- Graphene and Nanomaterials Applications
- Ubiquitin and proteasome pathways
- Antifungal resistance and susceptibility
- Corneal surgery and disorders
- RNA modifications and cancer
- Carbon and Quantum Dots Applications
- DNA and Nucleic Acid Chemistry
- Genetics and Neurodevelopmental Disorders
- Retinal Development and Disorders
- Ocular Infections and Treatments
- Drug Transport and Resistance Mechanisms
- Scientific and Engineering Research Topics
- Ocular Surface and Contact Lens
- Cancer-related gene regulation
- Nuclear Structure and Function
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Nanoparticles: synthesis and applications
Hiroshima University
2015-2024
Hiroshima Prefectural Hospital
2023-2024
Hiroshima University Hospital
2023
University of California, Davis
2019-2023
Yazaki (United States)
2023
Chiba University Hospital
2023
Research Institute for Bioscience and Biotechnology
2019
Abstract Homologous recombination plays essential roles in mitotic DNA double strand break (DSB) repair and meiotic genetic recombination. In eukaryotes, RAD51 promotes the central homologous-pairing step during homologous recombination, but is not sufficient to overcome reaction barrier imposed by nucleosomes. RAD54, a member of ATP-dependent nucleosome remodeling factor family, required promote RAD51-mediated pairing nucleosomal DNA. higher most nucleosomes form higher-ordered chromatin...
Histone exchange and histone post-translational modifications play important roles in the regulation of DNA metabolism, by re-organizing chromatin configuration. We previously demonstrated that variant H2A.Z-2 is rapidly exchanged at damaged sites after double strand break induction human cells. In yeast, small ubiquitin-like modifier (SUMO) modification H2A.Z involved damage response. However, whether SUMO regulates remains unclear. Here, we show SUMOylated a damage-dependent manner,...
Two-dimensional (2D) engineered nanomaterials are widely used in consumer and industrial goods due to their unique chemical physical characteristics. Engineered incredibly small capable of being aerosolized during manufacturing, with the potential for biological interaction at first-contact sites such as eye lung. The properties 2D that make them interest many industries may also cause toxicity towards epithelial cells. Using murine human respiratory cell culture models, we tested...
The dynamic exchange of histones alleviates the nucleosome barrier and simultaneously facilitates various aspects cellular DNA metabolism, such as repair transcription. In response to damage, acetylation Lys5 in histone variant H2AX, catalyzed by TIP60, plays a key role promoting exchange; however, detailed molecular mechanism still is unclear. Here, we show that TIP60 complex includes poly(ADP-ribose) polymerase 1 (PARP-1). PARP-1 required for rapid H2AX on chromatin at damage sites. It...
Homologous recombinational repair ( HR ) is one of the major systems for DNA double‐strand breaks. RAD 51 a key molecule in , and concentration cell nucleus increases after damage induction. However, mechanism that regulates intracellular distribution still unclear. Here, we show hCAS / CSE 1L associates with human cells. We found negatively nuclear protein level under normal conditions. also required to repress damage‐induced focus formation 51. Moreover, plays roles regulation activity...
Several ubiquitin-binding zinc fingers (UBZs) have been reported to preferentially bind K63-linked ubiquitin chains. In particular, the UBZ domain of FAAP20 (FAAP20-UBZ), a member Fanconi anemia core complex, seems recognize chains, in order recruit complex DNA interstrand crosslinks and mediate repair. By contrast, it is that attachment single Rev1, translesion polymerase, increases binding Rev1 FAAP20. To clarify specificity FAAP20-UBZ, we determined crystal structure FAAP20-UBZ with...
Abstract Purpose To evaluate the fovea in nanophthalmic eyes using spectral domain optical coherence tomography (SD-OCT) and OCT angiography (OCTA), to investigate relationship between macular microstructure visual acuity. Methods This is a retrospective case series of five patients. The foveal avascular zone (FAZ) area was measured superficial deep vascular layers with OCTA. thickness inner retinal layer (IRL) SD-OCT. ratio parafoveal IRL (fIRL/pIRL ratio) calculated. these parameters...
Linker histones bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration. Somatic germ cell-specific linker histone subtypes have been identified may distinct functions. In this study, we reconstituted containing human H1.2 H1T, as representative somatic histones, respectively, found that H1T forms less compacted chromatin, compared H1.2. An in vitro homologous pairing assay revealed weakly inhibited RAD51/RAD54-mediated although the H1 subtypes, H1.0,...
Corneal calcification typically progresses slowly but can occasionally advance rapidly. This report details severe stromal following repeat Descemet's stripping automated endothelial keratoplasty (DSAEK) in a 75-year-old patient with diabetes, hypertension, and prior ocular surgeries, including cataract surgery, intraocular lens extraction suturing, trabeculectomy. Persistent epithelial defects after the surgery led to rapid central within four weeks, significantly reducing visual acuity....
Chromosomal translocations are hallmarks of various types cancers and leukemias. However, the molecular mechanisms chromosome remain largely unknown. The ataxia-telangiectasia mutated (ATM) protein, a DNA damage signaling regulator, facilitates repair to prevent abnormalities. Previously, we showed that ATM deficiency led 11q23 translocation, most frequent abnormalities in secondary leukemia. Here, show ARP8, subunit INO80 chromatin remodeling complex, is phosphorylated after etoposide...
Matrin3 is a highly conserved inner nuclear matrix protein involved in multiple stages of RNA metabolism. Although may also play role DNA repair, its precise roles have remained unclear. In this study, we showed that the depletion led to decreased homologous recombination (HR) efficiency and increased radiation sensitivity cells. Matrin3-depleted cells impaired damage-dependent focus formation RAD51, key HR. These findings suggest promotes HR by regulating RAD51.
Acrophialophora fusispora is a filamentous fungus that found in soil and rarely infects humans. We herein report the first case of fungal keratitis caused by A. Japan present review literature on human infections with species. A 62-year-old Japanese male immunosuppressive therapy developed after removal corneal foreign body from his left eye. Voriconazole eye drops systemic for post-traumatic did not resolve infection, patient required therapeutic transplant. The isolate was identified as...
Histone modifications are catalyzed and recognized by specific proteins to regulate dynamic DNA metabolism processes. NSD2 is a histone H3 lysine 36 (H3K36)-specific methyltransferase that associated with both various transcription regulators repair factors. Specifically, it has been implicated in the of double-strand breaks (DSBs); however, role during DSB remains enigmatic. Here, we show does not accumulate at sites further mobilized formation. Using three different reporter systems, which...
Purpose: Corneal keratocyte-fibroblast-myofibroblast (KFM) transformation plays a critical role in corneal stromal wound healing. However, the impact of engineered nanomaterials (ENMs), found an increasing number commercial products, on this process is poorly studied. This study investigates effects metal oxide ENMs KFM vitro and vivo. Methods: Cell viability rabbit fibroblasts (RCFs) was tested following treatment with 11 at concentrations 0.5 to 250 µg/ml for 24 hours. Messenger RNA (mRNA)...
Abstract Genome-based functions are inseparable from the dynamic higher-order architecture of cell nucleus. In this context, repair DNA damage is coordinated by precise spatiotemporal controls that target and regulate machinery required to maintain genome integrity. However, mechanisms pair damaged with intact template for homologous recombination (HR) without illegitimate remain unclear. This report highlights intimate relationship between nuclear HR in mammalian cells. RAD51, key...
Abstract Histone modifications are catalyzed and recognized by specific proteins to regulate dynamic DNA metabolism processes. NSD2 is a histone H3 lysine 36 (H3K36)-specific methyltransferase that associates both with various transcription regulators repair factors. Specifically, it has been implicated in the of double-strand breaks (DSBs); however, role during DSB remains enigmatic. Here, we show does not accumulate at sites localization chromatin maintained even after formation. Using...
Hypercupremia-induced corneal copper deposition secondary to monoclonal gammopathy is rare and shows a characteristic opacity quite different from other causes of hypercupremia, such as Wilson's disease. This report describes case in patient with associated chronic lymphocytic leukemia. An 84-year-old man slowly progressive was referred our hospital. The present at least five years ago. patient's best-corrected visual acuity 20/25 OU (in both eyes) the initial visit Slit-lamp examination...