A5019907938- Glycosylation and Glycoproteins Research
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Carbohydrate Chemistry and Synthesis
- Cannabis and Cannabinoid Research
- Monoclonal and Polyclonal Antibodies Research
- Forensic Toxicology and Drug Analysis
- Alcohol Consumption and Health Effects
- Galectins and Cancer Biology
- Metabolomics and Mass Spectrometry Studies
- Fuel Cells and Related Materials
- Diet, Metabolism, and Disease
- Advanced Proteomics Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Electrocatalysts for Energy Conversion
- Political Economy and Marxism
- HIV Research and Treatment
- Biochemical effects in animals
- Psychedelics and Drug Studies
- Coding theory and cryptography
- Pharmacogenetics and Drug Metabolism
- Astrophysical Phenomena and Observations
- Global trade and economics
- Cryptography and Residue Arithmetic
- Metabolism and Genetic Disorders
University of Oxford
2015-2025
Science Oxford
1997-2024
The University of Adelaide
2006-2023
Linde (United States)
2018-2023
John Wiley & Sons (United States)
2018-2023
Terre des Hommes
2023
Hudson Institute
2023
Hanover College
2023
Liechtenstein Institute
2023
Film Independent
2022
Preface Prologue Part One: Universal Values 1. Kant's Anthropology and Geography 2. The Postcolonial Critique of Liberal Cosmopolitanism 3. Flat World Neoliberal Utopianism 4. New Cosmopolitans 5. Banality Geographical Evils Two: Knowledges 6. Reason 7. Spacetime the 8. Places, Regions, Territories 9. Nature Environment Epilogue: Theory Ruses Notes Bibliography Index
Ovarian cancer is the fourth most common in women Western world. In a pilot scale study, we highlight changes total serum glycome of patients with advanced ovarian that might shed insight into disease pathogenesis. These include increases levels core fucosylated, agalactosyl biantennary glycans (FA2) and sialyl Lewis x (SLex). To investigate further which proteins contribute to these alterations, developed technology analyze simultaneously glycosylation protein glycoforms contained single...
While the induction of a neutralizing antibody response against HIV remains daunting goal, data from both natural infection and vaccine-induced immune responses suggest that it may be possible to induce antibodies with enhanced Fc effector activity improved antiviral control via vaccination. However, specific features naturally induced HIV-specific allow for potent recruitment means by which these functions are regulated poorly defined. Because critically dependent on domain glycosylation,...
The envelope spike of HIV is one the most highly N-glycosylated structures found in nature. However, despite extensive research revealing essential functional roles infection and immune evasion, chemical glycans on native viral glycoprotein gp120—as opposed to recombinantly generated gp120—have not been described. Here, we report identity N-linked from primary isolates HIV-1 (clades A, B, C) simian immunodeficiency virus. MS analysis reveals a remarkably simple conserved virus-specific...
Glycoproteins present special problems for structural genomic analysis because they often require glycosylation in order to fold correctly, whereas their chemical and conformational heterogeneity generally inhibits crystallization. We show that the "glycosylation problem" can be solved by expressing glycoproteins transiently mammalian cells presence of N-glycosylation processing inhibitors, kifunensine or swainsonine. This allows correct folding glycoproteins, but leaves them sensitive...
Es posible pensar la crisis económica actual como el resultado de una matriz multidimensional causas sistémicas que operan acuerdo con un número finito dinámicas estructurales y variables geoeconómicas geopolíticas cuyas lógicas definen reproducción estructura social las pautas comportamiento generadas por capitalismo histórico o tan solo debemos interpretarla desajuste parcial sistema económico carece historia racionalidad sistémica cuya lógica únicamente puede reconstruirse concepto...
The HIV-1 envelope glycoprotein trimer is covered by an array of N-linked glycans that shield it from immune surveillance. high density on the surface imposes steric constraints limiting actions glycan-processing enzymes, so multiple under-processed structures remain specific areas. These oligomannose are recognized broadly neutralizing antibodies (bNAbs) not thwarted glycan but, paradoxically, target it. Our site-specific glycosylation analysis a soluble, recombinant (BG505 SOSIP.664) maps...
Secretory IgA (SIgA) is a multi-polypeptide complex consisting of secretory component (SC) covalently attached to dimeric containing one joining (J) chain. We present the analysis both N- and O-glycans on individual peptides from this complex. Based these data, we have constructed molecular model SIgA1 with all its glycans, in which Fab arms form T shape SC wrapped around heavy chains. The O-glycan regions (H) chains N-glycans adhesin-binding glycan epitopes including galactose-linked β1-4...
Murine thioglycollate-elicited peritoneal macrophages were cultured in the presence of a variety fatty acids added as complexes with bovine serum albumin. All tested taken up readily by cells and both neutral phospholipid fractions enriched acid provided medium. This generated range saturated, monounsaturated or polyunsaturated acids, including n-3 fish oil origin. Saturated enrichment enhanced macrophage adhesion to tissue culture plastic bacterial compared acids. Macrophages saturated...
Prion protein consists of an ensemble glycosylated variants or glycoforms. The enzymes that direct oligosaccharide processing, and hence control the glycan profile for any given glycoprotein, are often exquisitely sensitive to other events taking place within cell in which glycoprotein is expressed. Alterations populations sugars attached proteins can reflect changes caused, example, by developmental processes disease. Here we report normal (PrP(C)) pathogenic (PrP(Sc)) prion (PrP) from...
Abstract Comparative studies of the matrix‐assisted laser desorption ionization complex oligosaccharides were made using a range mono, di‐ and tri‐substituted benzoic acids, substituted coumarins cinnamic acids. The best results obtained with 2,5‐dihydroxybenzoic acid (2,5‐DHB). Detection limits this matrix in 100 fmol for high mannose sugars, some 2‐fold better than those α‐cyano‐4‐ hydroxycinnamic 10‐fold an earlier oligosaccharide matrix, 3‐amino‐4‐hydroxybenzoic acid. strongest signals...
Plant genetic engineering led to the production of plant-derived mAb (mAb P ), which provides a safe and economically feasible alternative current methods antibody in animal systems. In this study, heavy light chains human anti-rabies were expressed assembled planta under control two strong constitutive promoters. An alfalfa mosaic virus untranslated leader sequence Lys-Asp-Glu-Leu (KDEL) endoplasmic reticulum retention signal linked at N C terminus chain, respectively. was as effective...
Symbolic diagrams are commonly used to depict N- and O-linked glycans but there is no general consensus as how individual constituent monosaccharides or linkages shown. This article proposes a system that avoids ambiguities inherent in most other systems appropriate for both hand drawing computer applications. Constituent depicted by shapes modified show OAc, deoxy, etc. Linkage indicated the bond angle anomericity solid (beta) dashed (alpha) lines.
Negative ion spectra of N-linked glycans were produced by electrospray from a dilute solution the and various salts in methanol:water using Waters-Micromass Q-TOF Ultima Global tandem quadrupole/time-of-flight (Q-TOF) mass spectrometer. Stable anionic adducts formed with chloride, bromide, iodide, nitrate, sulphate, phosphate. Unstable that fragmented cross-ring cleavage reducing N-acetylglucosamine (GlcNAc) residue, fluoride, nitride, sulphide, carbonate, bicarbonate, hydroxide, acetate....
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of oligosaccharides and gangliosides normally causes loss sialic acid, particularly when alpha-cyano-4-hydroxycinnamic acid is used as the matrix. In addition, potential signal split because both positive and, to a greater extent, negative ions are formed while signals frequently complicated result partial alkali-salt formation. order stabilize moieties under MALDI conditions divert all into positive-ion mode, method...