A5044553741- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Metabolism and Genetic Disorders
- Peroxisome Proliferator-Activated Receptors
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Photoreceptor and optogenetics research
- Diet, Metabolism, and Disease
- Hemoglobin structure and function
- Metabolism, Diabetes, and Cancer
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Diet and metabolism studies
- Adipokines, Inflammation, and Metabolic Diseases
- CRISPR and Genetic Engineering
- Cholesterol and Lipid Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Lipids, and Metabolism
- Inflammatory Biomarkers in Disease Prognosis
- Diabetes and associated disorders
- FOXO transcription factor regulation
- Biochemical Acid Research Studies
- Wnt/β-catenin signaling in development and cancer
University of Bari Aldo Moro
2015-2024
Leipzig University
2013-2015
The Sense Innovation and Research Center
2014
Max Planck Society
2010
University of Milan
2008
The University of Melbourne
2008
California Institute of Technology
1997-2007
Institut Pasteur
1995-1999
University of Würzburg
1995
Istituto di Fisiologia Clinica
1994
Abstract Background Chronic renal disease (CKD) is characterized by complex changes in cell metabolism leading to an increased production of oxygen radicals, that, turn has been suggested play a key role numerous clinical complications this pathological condition. Several reports have focused on the identification biological elements involved development systemic biochemical alterations CKD, but abundant literature results fragmented and not exhaustive. Results To better define cellular...
The question of whether and to what extent the<i>in vivo</i> cytochrome <i>c</i> oxidase (COX) capacity in mammalian cells exceeds that required support respiration is still unresolved. In the present work, address this question, a newly developed approach for measuring rate COX activity, either as an isolated step or respiratory chain-integrated step, has been applied variety human cell types, including several tumor-derived semidifferentiated lines, well specialized removed from organism....
The metabolic control of respiration is still poorly understood, due mainly to the lack suitable approaches for studying it in vivo . Experiments on isolated mammalian mitochondria have indicated that a relatively small fraction each several components electron transport chain sufficient sustain normal O 2 consumption rate. These experiments, however, may not reflect accurately situation, mitochondrial essential cytosolic and use excess substrates vitro assays. An approach described here...
Peroxisome proliferator-activated receptor-γ coactivator 1-α (PGC1α) is a transcriptional able to up-regulate mitochondrial biogenesis, respiratory capacity, oxidative phosphorylation, and fatty acid β-oxidation with the final aim of providing more efficient pathway for aerobic energy production. In continuously renewed intestinal epithelium, proliferative cells in crypts migrate along villus axis differentiate into mature enterocytes, increasing their capacity finally undergoing apoptosis....
Successes in classical gene therapies have been achieved by placing a corrected copy of defective nuclear cells.A similar replacement approach for mutant mitochondrial genome is invariably linked to the use yet unavailable transfection vector.Here we show that DNA coupled covalently short leader peptide (chimera) can enter mitochondria via protein import pathway, opening new way gene-, antisense-RNAor antisense-DNA-delivery molecular therapies.The behavior purified chimera, composed...
Eukaryotic cells devoid of mitochondrial DNA (rho0 cells) were originally generated under artificial growth conditions utilizing ethidium bromide. The chemical is known to intercalate preferentially with the double-stranded thereby interfering enzymes replication machinery. Rho0 cell lines are highly valuable tools study human disorders because they can be utilized in cytoplasmic transfer experiments. However, mutagenic effects bromide onto nuclear cannot excluded. To foreclose this...
Inflammatory bowel disease (IBD) is a multifactorial intestinal disorder characterized by chronic inflammation. The etiology of IBD still unclear, although genetic, environmental and host factors have been associated to the disease. Extra-virgin olive oil (EVO) central component Mediterranean diet it decreases inflammation interfering with arachidonic acid NF-κB signaling pathways. Specifically, different components EVO are able confer advantages in terms health their site action. For...
An extensive analysis has been carried out of mitochondrial biochemical and bioenergetic properties fibroblasts, mostly skin-derived, from a large group subjects ranging in age between 20 wk fetal 103 yr. A striking age-related change observed fundamental process underlying biogenesis function was the very significant decrease rate protein synthesis individuals above 40 The endogenous respiration revealed range to 90 yr tendency uncoupling samples 60 surprising finding occurrence subgroup...
Mutations in the SPG7 gene encoding a mitochondrial protein termed paraplegin, are responsible for recessive form of hereditary spastic paraparesis. Only few studies have so far been performed large groups paraplegia (HSP) patients to determine frequency mutations. Here, we report result mutation screening conducted cohort 135 Italian HSP with identification six novel point mutations and one intragenic deletion. Sequence analysis deletion breakpoint, together secondary structure predictions...
In the present work, by titrating cytochrome<i>c</i> oxidase (COX) with specific inhibitor KCN, flux control coefficient and metabolic reserve capacity of COX have been determined in human saponin-permeabilized muscle fibers. presence substrates glutamate malate, a 2.3 ± 0.2-fold excess was observed ADP-stimulated skeletal This value found to be dependent on mitochondrial substrate supply. combined glutamate, succinate, which supported an approximately 1.4-fold higher rate respiration, only...
Development of hepatic steatosis and its progression to steatohepatitis may be the consequence dysfunction several metabolic pathways, such as triglyceride synthesis, very low-density lipoprotein (VLDL) secretion, fatty acid β-oxidation. Peroxisome proliferator-activated receptor γ coactivator-1β (PGC-1β) is a master regulator mitochondrial biogenesis oxidative metabolism, lipogenesis, (TG) secretion. Here we generated novel mouse model with constitutive activation PGC-1β studied role this...
Significance The mucosa of the small intestine is renewed completely every 3–5 d during entire lifetime through continuous steps proliferation, migration, and differentiation cells from crypt site on bottom to villus top mucosa. factors that regulate enterocyte lifespan aging are special interest as related colon cancer susceptibility. Here, using genetically modified gain- loss-of-function models, we present importance mitochondrial respiration chain reactive oxygen species homeostasis in...
Abstract While aberrant cancer cell growth is frequently associated with altered biochemical metabolism, normal mitochondrial functions are usually preserved and necessary for full malignant transformation. The transcription factor FoxO3A a key determinant of homeostasis, playing dual role in survival/death response to metabolic stress therapeutics. We recently described novel arm the AMPK-FoxO3A axis cells upon nutrient shortage. Here, we show that metabolically stressed cells, recruited...
The H + /e − stoichiometry of protonmotive cytochrome c oxidase, isolated from bovine heart mitochondria and reconstituted in liposomes, has been determined by making use direct spectrophotometric measurements the initial rates e flow translocation. It is shown that ←H ratio for redox‐linked proton ejection oxidase varies around 0 to a maximum 1 as function rate overall electron complex.
The peroxisome proliferator-activated receptor γ (PPARγ) coactivator-1β (PGC-1 β) is a master regulator of mitochondrial biogenesis and oxidative metabolism as well antioxidant defense. Specifically, in the liver, PGC-1β also promotes de novo lipogenesis, thus sustaining cellular anabolic processes. Given relevant pathogenic role fatty acid hepatocarcinoma (HCC), here we pointed to putative novel transcriptional player development progression HCC. For this purpose, generated both...
Abstract Background & Aims Metabolic dysfunction‐associated steatohepatitis (MASH) is a growing cause of chronic liver disease, characterized by fat accumulation, inflammation and fibrosis, which development depends on mitochondrial dysfunction oxidative stress. Highly expressed in the during fasting, peroxisome proliferator‐activated receptor‐γ coactivator‐1α (PGC‐1α) regulates metabolism. Given relevant role function MASH, we investigated relationship between PGC‐1α steatohepatitis....