Infectious bursal disease virus (IBDV), a member of the Birnaviridae, specifies two genomic double-stranded RNAs, segment A and B. Segment encodes 110 kDa polyprotein which is processed into proteins VP2, VP3 VP4. second open reading frame (ORF), designated ORF A-2, immediately preceding partially overlapping protein gene has also been described. After prokaryotic expression this immunization rabbits with expressed we obtained reagents that allowed identification A-2 product in IBDV-infected...

Summary The electrophoretic mobilities of the two genome segments and structural polypeptides chicken strain Cu-1 (serotype I) turkey isolate 23/82 II) infectious bursal disease virus were compared. There is a close antigenic relationship between smaller major proteins (32K) both strains. Neutralizing monoclonal antibodies are induced by larger protein (40K in Cu-1) which differentiates serotypes. 40K also has epitopes do not induce neutralizing common to evidence that region responsible for...

Bovine papilloma virus (BPV) appears to be the etiological agent of common equine connective tissue tumors. We investigated physical state viral DNA within such tumors and found no indication for integration into host genome. The BPV genomes were present as free circular episomes. Two sarcoids shown contain multiple copies type 1 (BPV-1) DNA. When digested with several single-cut restriction enzymes, there only form III BPV-1 sequences could revealed. One contained, apart from wild-type DNA,...

The aim of this work was the selection and comparison representative infectious bursal disease virus (IBDV) strains. Nine strains IBDV, isolated at different times from geographic regions Europe China, were characterized. Batches all prepared following standardized protocols checked for absence contaminating viruses. Criteria used their characterization were: (i) nucleotide sequence VP2 variable region, (ii) binding to a panel neutralizing monoclonal antibodies in antigen capture...

The genomic region coding for the antigenic structure responsible induction of neutralizing antibodies was localized in central variable VP2 gene by comparing nucleotide sequence five escape mutants derived from standard infectious bursal disease virus strain Cu-1. Exchange a single amino acid at one prominent hydrophilic parts this proved to be sufficient altering properties. reactivity with peptides expressed vitro encompassing both areas suggests that entire is engaged formation...

It has previously been shown that infectious bursal disease virus is a naked icosahedral particle with diameter of about 60 nm and genome consisting two segments double-stranded RNA (Müller et al., J. Virol. 31:584-589, 1979). One the major structural polypeptides (molecular weight, 40,000) this could not be found in lysates infected cells; it derived from precursor polypeptide demonstrable inside cells relatively large quantities seems to processed during assembly or later. The molecule...

This study was carried out to detect differences in locomotion and feeding behavior lame (group L; n = 41; gait score ≥ 2.5) non-lame C; 12; ≤ 2) multiparous Holstein cows a cross-sectional design. A model for automatic lameness detection created, using data from accelerometers attached the hind limbs noseband sensors head. Each cow's videotaped scored on 5-point scale before after period of 3 consecutive days behavioral recording. The mean value independent experienced observers taken as...

Detecting lame cows is important in improving animal welfare. Automated tools are potentially useful to enable identification and monitoring of cows. The goals this study were evaluate the suitability various physiological behavioral parameters automatically detect lameness dairy housed a cubicle barn. Lame suffering from claw horn lesion (sole ulcer or white line disease) one same hind limb (n=32; group L) 10 nonlame healthy (group C) included study. Lying standing behavior at night by...

Journal Article Nucleotide sequence of infectious bursal disease virus genome segment A delineates two major open reading frames Get access U. Spies, Spies Institut für Virologie, Justus-Liebig-Universität GiessenFrankfurter Strasse 107, D-6300 Giessen, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Müller, Müller Becht Nucleic Acids Research, Volume 17, Issue 19, 11 October 1989, Page 7982, https://doi.org/10.1093/nar/17.19.7982 Published: 1989...

A virus (MnPV) with the structural characteristics of papilloma viruses was isolated from benign and malignant proliferations adult animals inbred line 'GRA Giessen' Mastomys natalensis. The particles can be banded in CsCl gradients at densities 1.34 g/ml (full particles) 1.29 (empty particles). DNA has a buoyant density 1.7104 exist three different conformations (supercoiled circular, nicked circular linear), sedimentation values which have been determined as 23 to 24S, 16 17S 14 15S,...

Summary Chicken anaemia virus (CAV) was detected in the bursa of Fabricius a 4‐week‐old chicken obtained from an outbreak acute infectious bursal disease Bangladesh. Repeated attempts to grow this MDCC‐MSB1 cells were not successful. A full‐length PCR amplicon genome strain, designated as BD‐3 CAV, cloned and sequenced. The complete nucleotide sequence deduced amino acid compared with those 12 other CAV strains. genetic analysis sequences VP1 indicated possible existence groups among...

Polyomaviruses of birds are aetiological agents acute inflammatory diseases in non-immunocompromised hosts, which is contrast to mammalian polyomaviruses. VP4, an additional structural protein encoded by the viral genomes known avian polyomaviruses, has been suggested contribute pathogenicity through loss cells following induction apoptosis. Four distinct bird polyomaviruses have identified so far, infect crows, finches, geese and parrots. Using broad-spectrum PCR, a novel polyomavirus,...

Pathogenic serotype 1 strains of infectious bursal disease virus (IBDV) replicate efficiently in lymphoid cells the bursa Fabricius chicken. Lymphoid other organs are not susceptible. Apathogenic 2 do or cells. Chicken embryo fibroblasts (CEF), however, either serotype. Binding studies showed that both IBDV serotypes bind to isolated from bursa, thymus spleen, indicating restriction replication B is determined by presence specific receptor sites. The specificity binding was demonstrated...

Two Muscovy duck reoviruses, strains y1/79 and 1625/87, were investigated with regard to their genome organization, polypeptide pattern, serotype specificity, pathogenicity. Electrophoretic analysis of the revealed migration pattern avian reoviruses. In spite general conformity, great polymorphism was detected in electrophoretic mobility individual segments two sharing only three identical size (L1, M2, M3). Only one segment (M3) migrated into same position as corresponding prototype chicken...

A molecularly cloned, tissue culture-adapted infectious bursal disease virus (BD-3tc) was generated from a very virulent strain by the reverse genetics approach following site-directed mutagenesis (Q253H and A284T in VP2). The pathogenicity of BD-3tc tested commercial chickens. wild-type (BD-3wt) cloned parental (BD-3mc) were included for comparison. subclinical course disease, with delayed milder pathological lesions followed quick follicular regeneration bursa Fabricius BD-3tc-inoculated...

Three isolates of infectious bursal disease virus (IBDV), obtained from chickens in Bangladesh 1999 and designated as BD 1/99, 2/99 3/99, were characterized. In an antigen capture–enzyme‐linked immunosorbent assay using a panel VP2‐specific neutralizing monoclonal antibodies (mAb), all three showed mAb‐binding profile similar to that very virulent IBDV (vvIBDV) strains. contrast the classical strains, they did not react with mAb 3 4. Molecular characterization was performed by direct...