Abstract The rye flour is, together with the wheat flour, basic ingredient used in traditional bread baking. grain contains many compounds significant impacts on consumer. Considering that, various biologically active phytochemicals were determined extracts from mature grains of 19 genotypes ( Secale cereale L.). content total phenols, flavonoids, phenolic acids and thiols, as well antioxidant activities inhibitory against trypsin, thrombin, urokinase analyzed by spectrophotometric methods....

An original engine has been developed for the frugal extraction of plant extractive compounds with an accelerating effect. It operates on principle simultaneously combining several physical factors (sonication, temperature, and electrical field) to promote extraction. By applying this engine, extract samples were obtained significantly enhanced dry matter content as well bioactivity, particularly in antioxidant, antibacterial, enzyme inhibition, anti-inflammatory activities. This device was...

Abstract An assay was conducted for the determination of malondialdehyde (MDA) levels in broiler meat. The method involves extraction tissues with trichloroacetic acid (TCA) and reaction TCA extract 2,4-dinitrophenylhydrazine (DNPH). After separation MDA-DNPH complex using a solid-phase C18 column, samples were eluted 1 mL acetonitrile. Aliquots 20 μL acetonitrile analyzed by liquid chromatography on reversed-phase column (3 μm) UV detection. products isocratically mobile phase containing...

Avenanthramides play a role as phytoalexins in oat.Recently, various bioactivities have been found avenanthramides an antioxidant activity, anti-inflammatory, anti-atherogenic and anti-proliferative effect.In this study, the optimal conditions for extraction of 2c, 2p 2f from oat grain, cultivar Avenuda, were determined using response surface methodology (RSM).The effects three independent variables (methanol concentration, temperature time) on yield aventhramides investigated.A second-order...

Concentrations of total mercury and selenium were determined in 49 42 muscle tissue samples, respectively, six species predatory freshwater fish, dace (Leuciscus leuciscus), pike perch (Sander lucioperca), (Esox lucius), European catfish (Silurus glanis), rainbow trout (Oncorhynchus mykiss) asp (Aspius aspius). Muscle concentration did not correlate with the corresponding (R² < 0.198) all examined fish species. There was an inverse correlation between ratio Se/Hg content tissues dace, perch,...

The presence of sulphadimidine residues in eggs after a peroral administration Sulfadimidin PG plv. sol. ad us, vet. (120 mg/hen/day) to laying hens was studied. Premi®Test, four-plate microbiological method, and HPLC were used for the detection residues. Positive findings Four-plate test (FPT) confirmed by results Premi®Test. Using FPT, absence 72 hours last administration. has been detected Premi®Test within 8 days FPT 3 As compared with reported false-negative five (kappa < 0.6)....

Abstract Oats are important cereals. a good source of protein and lipids, polyphenolics, phenolic acids, flavonoids avenanthramides. Avenanthramides is group, which unique in oats have antioxidant activity, anti-inflammatory, anti-atherogenic anti-proliferative effect. The aim study determination the majoritarian avenanthramides (2c, 2p 2f) acids (p-coumaric ferulic) selected varieties oat (Avena sativa L.) grown two consecutive years using HPLC method. were exposed to ultrasound supported...

A high prevalence of genetic polymorphisms increases sensitivity to warfarin therapy. In this study, we investigated 47 patients with effective long-term therapy by well-controlled monitoring International Normalised Ratio (INR). All were tested for gene VKORC1, CYP2C9*C2, and CYP2C9*C3, which used a dose calculation employing program www.WarfarinDosing.org. The main goal was investigate whether the doses determined INR are in accordance calculated according pharmacogenetic algorithm. For...

Abstract Sage is medicinal plant, known for its antioxidant and anti-inflammatory effects. Eight extract samples were tested in this study: from Salvia officinalis L. varieties two different geographical localities (Jaslovské Bohunice Pobedim, Slovakia), L., variety “bicolor”, “purpurescens”, apiana , divinorum callus cultures of sclarea aethiopis The highest values composite parameters observed . It can be concluded that prepared sage are rich on polyphenolic acids (2 950±265 μg.mL −1...

Biogenic amines are low-molecular-weight nitrogen compounds that formed primarily by the decarboxylation of amino acids microbial enzymes. These active substances found mainly in various types food or beverages. At certain concentrations, biogenic essential for many physiological functions but toxic if consumed large quantities. Therefore, development and optimization methods sensitive to determine these very much needed. The determination beverages most commonly used separation with...

The aim of this study was to observe the effectiveness disinfection on a broiler farm and in plant processing poultry from farm. disinfected with preparation based peracetic acid while quarternary ammonium salt used plant. We evaluated swabs taken surfaces, which come into contact broilers meat. Results by standard microbiological swabbing method were results ATP-bioluminescence method. examination included total counts microorganisms, coliform count moulds. When using plate we found that 0%...