A5048922273- Cellular Mechanics and Interactions
- Corneal Surgery and Treatments
- Retinal Development and Disorders
- Developmental Biology and Gene Regulation
- Marine Invertebrate Physiology and Ecology
- Cell Adhesion Molecules Research
- Zebrafish Biomedical Research Applications
- Axon Guidance and Neuronal Signaling
- Cell Image Analysis Techniques
- Planarian Biology and Electrostimulation
- Epigenetics and DNA Methylation
- Erythrocyte Function and Pathophysiology
- Connexins and lens biology
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Connective tissue disorders research
- Echinoderm biology and ecology
University of Florida
2021
University of Utah
2016-2020
Huntsman Cancer Institute
2016
Organogenesis requires precise interactions between a developing tissue and its environment. In vertebrates, the eye is surrounded by complex extracellular matrix as well multiple mesenchymal cell populations. Disruptions to either or periocular mesenchyme can cause defects in early development, yet many cases, underlying mechanism unknown. Here, using multidimensional imaging computational analyses zebrafish, we establish that movements optic cup require neural crest. Ultrastructural...
Epithelia provide a critical protective barrier for our organs and are also the sites where most carcinomas form. Most studies on epithelia use cell culture or organisms high-resolution live imaging is inaccessible without invasive techniques. Here, we introduce developing zebrafish epidermis as an excellent in vivo model system studying living epithelium. We developed tools to fluorescently tag specific epithelial types express genes mosaic fashion using five GAL4 lines identified from...
The vertebrate eye forms via a complex set of morphogenetic events. optic vesicle evaginates and undergoes transformative shape changes to form the cup, in which neural retina retinal pigmented epithelium enwrap lens. It has long been known that complex, glycoprotein-rich extracellular matrix layer surrounds developing cup throughout process, yet functions its specific molecular components have remained unclear. Previous work established role for laminin particular steps development,...
Epithelial wound healing requires a complex orchestration of cellular rearrangements and movements to restore tissue architecture function after injury. While it is well known that mechanical forces can affect morphogenesis patterning, how the biophysical cues generated injury influence behaviors during repair not understood. Using time-lapse confocal imaging epithelial tissues in living zebrafish larvae, we provide evidence localized increases crowding closure promote extrusion nonapoptotic...
Epithelial morphogenesis, a fundamental aspect of development, generates 3-dimensional tissue structures crucial for organ function. Underlying morphogenetic mechanisms are, in many cases, poorly understood, but mutations that perturb development can affect epithelial cell shape and orientation – difficult features to quantify three dimensions. The basic structure the eye is established via morphogenesis: embryonic optic cup, retinal progenitor epithelium enwraps lens. We previously found...
Abstract Epithelial wound healing requires a complex orchestration of cellular rearrangements and movements to restore tissue architecture function after injury. While it is well-known that mechanical forces can affect morphogenesis patterning, how the biophysical cues generated injury influence behaviors during repair not well understood. Using time-lapsed confocal imaging epithelial tissues in living zebrafish larvae, we provide evidence localized increases crowding closure promote...
Abstract The interactions between an organ and its surrounding environment are critical in regulating development. In vertebrates, neural crest mesodermal mesenchymal cells have been observed close to the eye during development, mutations affecting this periocular mesenchyme can cause defects early yet underlying mechanism has unknown. Here, using timelapse microscopy four-dimensional cell tracking zebrafish, we establish that genetic loss of impairs movements within optic vesicle. At...
Abstract Epithelial morphogenesis, a fundamental aspect of development, generates 3-dimensional tissue structures crucial for organ function. Underlying morphogenetic mechanisms are, in many cases, poorly understood, but mutations that perturb development can affect epithelial cell shape and orientation – difficult features to quantify three dimensions. The basic structure the eye is established via morphogenesis: embryonic optic cup, retinal progenitor epithelium enwraps lens. We previously...
Precise and stereotyped 3‐dimensional tissue structure, established during the process of morphogenesis, is essential for proper organ function. In visual system, aberrant structural development embryonic eye can lead to impairment blindness. Despite increasing knowledge pathways controlling differentiation many cell types in eye, we still lack a basic understanding mechanisms its morphogenesis. Using combination 4‐dimensional timelapse imaging, computational approaches, zebrafish genetics,...
Abstract Morphogenesis, the formation of three-dimensional organ structures, requires precise coupling genetic regulation and complex cell behaviors. The networks governing many morphogenetic systems, including that embryonic eye, are poorly understood. In zebrafish, several forward screens have sought to identify factors regulating eye development. These often look for defects at stages after optic cup is formed when retinal neurogenesis under way. This approach can make it difficult...