A5054198449- Blood Pressure and Hypertension Studies
- Hormonal Regulation and Hypertension
- Ion Transport and Channel Regulation
- Acute Ischemic Stroke Management
- Electrolyte and hormonal disorders
- Renal function and acid-base balance
- Cerebrovascular and Carotid Artery Diseases
- Nitric Oxide and Endothelin Effects
- Protein Kinase Regulation and GTPase Signaling
- Sodium Intake and Health
- Cardiac, Anesthesia and Surgical Outcomes
- Renal and Vascular Pathologies
- Diet and metabolism studies
- Pharmaceutical Practices and Patient Outcomes
- Renin-Angiotensin System Studies
- Health and Well-being Studies
- Eicosanoids and Hypertension Pharmacology
- Traumatic Brain Injury and Neurovascular Disturbances
- Adipose Tissue and Metabolism
- Erythrocyte Function and Pathophysiology
- Heart Rate Variability and Autonomic Control
- Ion channel regulation and function
- Potassium and Related Disorders
- Health Sciences Research and Education
- Metabolism, Diabetes, and Cancer
University of Padua
2014-2023
Policlinico Universitario di Catania
1998-2017
Ospedale San Paolo
2013-2014
University of Cagliari
2009
Institute of Clinical and Experimental Medicine
2009
Vall d'Hebron Institut de Recerca
2009
Universitat Autònoma de Barcelona
2009
Sapienza University of Rome
2008
University of Trieste
2007
Clinical Emergency Hospital Bucharest
2007
Sirtuins (SIRTs) are NAD(+)-dependent deacetylases that regulate metabolism and life span. We used peripheral blood mononuclear cells (PBMCs) to determine ex vivo whether insulin resistance/metabolic syndrome influences SIRTs. also assessed the potential mechanisms linking metabolic alterations SIRTs in human monocytes (THP-1) vitro.SIRT1-SIRT7 gene protein expression was determined PBMCs of 54 subjects (41 with normal glucose tolerance 13 syndrome). Insulin sensitivity by minimal model...
Primary aldosteronism (PA) causes cardiovascular damage in excess to the blood pressure elevation, but there are no prospective studies proving a worse long-term prognosis adrenalectomized and medically treated patients. We have, therefore, assessed outcome of PA patients according treatment mode PAPY study (Primary Aldosteronism Prevalence Hypertension) patients, 88.8% whom were optimally with primary (essential) hypertension (PH), rest had assigned medical therapy (6.4%) or adrenalectomy...
Hyperglycemia is the main determinant of long-term diabetic complications, mainly through induction oxidative stress. NAD(P)H oxidase a major source glucose-induced In this study, we tested hypothesis that rosiglitazone (RSG) able to quench stress initiated by high glucose prevention activation.Intracellular ROS were measured using fluoroprobe TEMPO-9-AC in HUVECs exposed control (5 mmol/L) and moderately (10 concentrations. AMPK activities determined Western blot. We found 10 mmol/L...
A silent polymorphism (+1166 A/C single-nucleotide polymorphism) localized in the 3'-UTR (untranslated region) of human angiotensin II type-1 receptor (AT1R) has been associated with hypertension and cardiovascular complications. The +1166 is recognized by a specific microRNA-155 (miR-155), which base-pairing complementary A-allele but not mutant C allele. Aim our study was to investigate interplay between miR-155 AT1R protein expression.Sixty-four subjects were selected for from cohort...
Objective: To investigate the role of ouabain in human hypertension and to establish whether immunoreactive endogenous is secreted by adrenal gland under influence dopaminergic regulation. Methods: We measured plasma levels immunoassay, together with other variables, including renin activity aldosterone levels, 91 clinically selected hypertensives 19 healthy volunteers. also venous blood effect DA2 receptor blockade stimulation. After a thorough clinical evaluation, 64 patients were...
RGS2 (regulators of G-protein signaling) is a negative regulator Galphaq protein signaling, which mediates the action several vasoconstrictors. RGS2-deficient mouse line exhibits hypertensive phenotype and prolonged response to vasoconstrictors.To compare expression in peripheral blood mononuclear cells (PBMs) cultured fibroblasts from normotensive subjects patients.PBMs were isolated 100 controls 150 essential hypertensives. Additionally, skin biopsy 11 normotensives 12 hypertensives up...
Background Data on the performance of tests used to confirm diagnosis primary aldosteronism (PA) are limited. Objective To prospectively investigate accuracy saline infusion test (SIT). Methods Three hundred and seventeen (26.9%) out 1125 patients screened in PAPY study underwent measurement plasma aldosterone, cortisol renin activity after 2 l isotonic intravenously over 4 h. They comprised with a baseline aldosterone/renin ratio (ARR) > 40 one every four not fulfilling such criterion. The...
Current guidelines recommend use of the aldosterone-renin ratio (ARR) for case detection primary aldosteronism followed by confirmatory tests to exclude false-positive results from further diagnostic workup. We investigated hypothesis that this could be unnecessary in patients with a high ARR value if quantitative information carried is taken into due consideration.We interrogated 2 large data sets prospectively collected studied same predefined protocol, which included captopril challenge...
In acute ischemic stroke, a transient blood pressure (BP) elevation is common, but the best management still unknown. Therefore, we investigated retrospectively relationship between BP after stroke and neurological outcome (evaluated by means of National Institutes Health Stroke Scale score at day 7).The medical records 92 consecutive patients with aged 47 to 96 years, were examined. Blood was measured on admission, 4 times during first 24 hours, 3 daily for days, twice 7 (or discharge)....
Because insulin shows an antinatriuretic effect in healthy humans, therapy resulting circulating hyperinsulinemia may lead to sodium retention and turn hypertension individuals with insulin-dependent diabetes mellitus (IDDM). Moreover, it has been proved that atrial natriuretic peptide (ANP) plays a major role modulating natriuresis humans. This study investigated the relationship between ANP metabolism normotensive hypertensive IDDM subjects compared control groups of nondiabetic subjects....
Regulator of G protein signaling-2 (RGS-2) plays a key role in the protein-coupled receptor (GPCR) angiotensin II (Ang II) signaling. NO and cGMP exert vasodilating action also through activation binding to RGS-2 dependent kinase 1-α, which phosphorylates dephosphorylates myosin light chain. In Bartter’s/Gitelman’s patients (BS/GS) Ang related signaling vasomotor tone are blunted. Experiments were planned explore whether may play BS/GS vascular hyporeactivity. metabolites urinary excretion...
Hyperglycemia determines the vascular complications of diabetes through different mechanisms: one these is excessive activation isoform β2 protein kinase C (PKC-β2). Metformin, a widely used antidiabetic agent, associated with decreased cardiovascular mortality in obese type 2 diabetic patients. Therefore, we assessed role metformin glucose-induced PKC-β2 and determined mechanism its effect human umbilical venous endothelial cells grown to either normo- (5 mmol/l) or hyperglycemia (10...
Abstract Introduction Our aim was to evaluate the role of B-type natriuretic peptide (BNP) percentage variations at 24 hours and discharge compared its value admission in order demonstrate predictive for outcomes patients with acute decompensated heart failure (ADHF). Methods This a multicenter Italian (8 centers) observational study (Italian Research Emergency Department: RED). 287 ADHF were studied through physical exams, lab tests, chest X Ray, electrocardiograms (ECGs) BNP measurements,...
Activation of protein kinase C (PKC) by hyperglycemia is implicated in the pathogenesis long-term diabetic complications. Monocyte activation and transformation into macrophages a key step atherosclerotic process. Therefore, this study, we sought to determine 1) effect on monocyte PKC activity distribution Ca2+-dependent diacylglycerol-sensitive isoforms; 2) whether effects these parameters are determined per se, independent state. The studies were performed 19 type 2 patients 14 control...
Oxidative stress may be involved in the development of vascular complications associated with diabetes; however, molecular mechanism responsible for increased production free radicals diabetes remains uncertain. Therefore, we examined whether acute hyperinsulinemia increases and this condition affects proliferative extracellular signal-regulated kinase (ERK-1 -2) signaling human fibroblasts vitro. Insulin treatment significantly intracellular superoxide anion (O2−) production, an effect...
The aim of the present study was to determine whether inhibition reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] oxidase and various superoxide generating systems could affect collagen production, mRNA protein expression types I III in control angiotensin II-treated cardiac fibroblasts.Cardiac fibroblasts from passage 2 normal male adult rats were cultured confluency incubated serum-free Dulbecco's modified Eagle's medium for 24 h. cells then preincubated with(out) tested...
Primary aldosteronism (PA) is the most common endocrine cause of curable hypertension, but no single test unequivocally identifies it. Accordingly, we investigated usefulness a logistic multivariate discriminant analysis (MDA) approach for PA screening.Generation MDA function based on retrospective biochemical tests in large cohort referred patients with/without confirmed Conn's adenoma (CA), followed by prospective validation model.We 574 selected hypertensives: 206 (32 with and 174 without...
A familial predisposition to arterial hypertension has recently been suggested as one important component of the susceptibility diabetic kidney disease. Sodium-lithium countertransport activity, a marker risk for essential hypertension, found be increased in patients with overt nephropathy. We have measured red blood cell sodium-lithium counter-transport activity 36 microalbuminuric insulin-dependent patients, group at high progression clinical nephropathy and cardiovascular disease,...
In patients with cyclosporine-induced hypertension, upregulation of the nitric oxide system and oxidative stress were shown, which could induce remodeling, chronic rejection by increasing catabolism. However, it is still debated whether cyclosporine tacrolimus exert a different action. The aim current study was to compare effects on markers endothelial dysfunction in kidney transplant posttransplant hypertension. Monocyte p22, NADH/NADPH subunit, transforming growth factor-beta (TGF-beta),...
<i>Background:</i> Intravenous (i.v.) thrombolysis with rt-PA within 3 h from symptom onset is the only approved treatment of pharmacological revascularization in acute ischemic stroke. However, little information exists on its use elderly patients, particular those aged >80 years, who at present are excluded treatment. <i>Methods:</i> In a multicenter Italian study i.v. thrombolysis, patients years (n = 41) were compared ≤80 207) regarding percentage symptomatic...