A5063743181- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Cytomegalovirus and herpesvirus research
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Childhood Cancer Survivors' Quality of Life
- Genetic Syndromes and Imprinting
- Acute Myeloid Leukemia Research
- Immune responses and vaccinations
- Acute Lymphoblastic Leukemia research
Josep Carreras Leukaemia Research Institute
2018-2024
Universitat Autònoma de Barcelona
2018
Abstract Activation of the p53 tumor suppressor triggers a transcriptional program to control cellular response stress. However, molecular mechanisms by which controls gene transcription are not completely understood. Here, we uncover critical role spatio-temporal genome architecture in this process. We demonstrate that drives direct and indirect changes compartments, topologically associating domains, DNA loops prior one hour its activation, escort program. Focusing on p53-bound enhancers,...
ABSTRACT DNA methylation is traditionally associated with gene silencing, but its causal relationship and role in shaping cell fate decisions still need to be fully elucidated. Here, we conducted a genome-wide analysis investigate the between expression at regulatory regions human immune cells. By utilizing CRISPR-dCas9 editing tools, successfully established cause-and-effect levels of promoter Interleukin1-receptor antagonist ( IL1RN ) expression. Notably, observed that modifying status...
Reprogramming somatic cells into induced pluripotent stem (iPSCs) requires activation of the pluripotency network and resetting epigenome by erasing epigenetic memory state. In female mouse cells, a critical reprogramming step is reactivation inactive X chromosome. Despite its importance, systematic understanding regulatory networks linking X-reactivation missing. Here, we reveal important pathways for acquisition using genome-wide CRISPR screen during neural precursor to iPSC reprogramming....
Abstract Reprogramming somatic cells into induced pluripotent stem (iPSCs) requires activation of the pluripotency network and resetting epigenome by erasing epigenetic memory state. In female mouse cells, a critical reprogramming step is reactivation inactive X chromosome. Despite its importance, systematic understanding regulatory networks linking X-reactivation missing. Here we reveal pathways important for iPSC using genome-wide CRISPR screen. particular, discover that interferon γ...
Activation of the p53 tumor suppressor triggers a transcriptional program to control cellular response stress. However, molecular mechanisms by which controls gene transcription are not completely understood. Here, using multi-omics integration framework, we uncover critical role spatio-temporal genome architecture in this process. We demonstrate that drives direct and indirect changes compartments, topologically associating domains DNA loops within minutes its activation, escort along time....