A5071562196- Endoplasmic Reticulum Stress and Disease
- Liver Disease Diagnosis and Treatment
- Antioxidant Activity and Oxidative Stress
- Graphene and Nanomaterials Applications
- Paraoxonase enzyme and polymorphisms
- Nanoparticle-Based Drug Delivery
- Lipid metabolism and biosynthesis
- Meat and Animal Product Quality
- Carbon and Quantum Dots Applications
- Computational Drug Discovery Methods
- Graphene research and applications
- Hibiscus Plant Research Studies
- Heat shock proteins research
- Genomics, phytochemicals, and oxidative stress
- Identification and Quantification in Food
- Fullerene Chemistry and Applications
- Galectins and Cancer Biology
- Cholesterol and Lipid Metabolism
- Curcumin's Biomedical Applications
- Redox biology and oxidative stress
- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Edible Oils Quality and Analysis
Instituto de Investigación Sanitaria Aragón
2021-2024
Universidad de Zaragoza
2020-2024
Carbon nanomaterials have attracted increasing attention in biomedicine recently to be used as drug nanocarriers suitable for medical treatments, due their large surface area, high cellular internalization and preferential tumor accumulation, that enable these transport chemotherapeutic agents preferentially sites, thereby reducing toxic side effects. However, there are widespread concerns on the inherent cytotoxicity of carbon nanomaterials, which remains controversial this day, with...
Non-alcoholic fatty liver disease (NAFLD) is currently a growing epidemic that can lead to cirrhosis and hepatic cancer when it evolves into non-alcoholic steatohepatitis (NASH), gap not well understood. To characterize this disease, pigs, considered be one of the most similar human experimental animal models, were used. date, all swine-based settings have been carried out using rare predisposed breeds or long-term experiments. Herein, we fully describe new swine model for initial reversible...
Non-alcoholic fatty liver disease or steatosis is an accumulation of fat in the liver. Increased amounts non-esterified acids, calcium deficiency, insulin resistance may disturb endoplasmic reticulum (ER) homeostasis, which leads to abnormal misfolded proteins, activating unfolded protein response. The ER primary location site for chaperones like thioredoxin domain-containing 5 (TXNDC5). Glutathione participates cellular oxidative stress, and its interaction with TXNDC5 decrease disulfide...
Squalene is a key minor component of virgin olive oil, the main source fat in Mediterranean diet, and had shown to improve liver metabolism rabbits mice. The present research was carried out find whether this effect conserved porcine model hepatic steatohepatitis search for lipidomic changes involved. current study revealed that 0.5% squalene supplementation steatotic diet month led accumulation decreased triglyceride content as well area lipid droplets without influencing cholesterol or...
Introduction Pulsed electric field (PEF) has been used for improving extraction of extra virgin olive oil (EVOO). However, the biological changes induced by consumption pulsed field-obtained (PEFEVOO) have not studied yet. Materials and methods EVOO oils from Empeltre variety were prepared standard (STD) cold pressure method involving crushing olives, malaxation decanting this procedure including an additional step PEF treatment. Chemical analyses done. Male female Apoe -deficient mice...
Hepatic thioredoxin domain-containing 5 (TXNDC5) is a member of the protein disulfide isomerase family found associated with anti-steatotic properties squalene and located in endoplasmic reticulum lipid droplets. Considering that latter are involved hepatic accumulation, present research was aimed to investigate role TXNDC5 on management mice AML12 cell line. Wild-type TXNDC5-deficient (KO) were fed Western diets or without 1% supplementation for 6 weeks. In males, but not females, absence...
Squalene, a triterpene found in extra virgin olive oil, has therapeutic properties diseases related to oxidative stress, such as cancer. However, its hydrophobic nature and susceptibility oxidation limit bioavailability outside of oil. To expand applications, alternative delivery methods are necessary. The objective the present study was examine impact squalene encapsulated PLGA (poly(lactic-co-glycolic) acid) nanoparticles (PLGA + Sq) on proliferation human colon carcinoma Caco-2 cells,...
Background and aim: The type amount of dietary protein has become a topic renewed interest in light their involvement metabolic diseases, atherosclerosis thrombosis. However, little attention been devoted to the effect avian proteins despite wide human consumption. aim was investigate influence chicken turkey as sources compared with that soybean on fatty liver disease. Methods results: To this purpose, male female Apoe-deficient were fed purified Western diets differing for 12 weeks. After...
Thioredoxin domain containing 5 (TXNDC5) is a protein disulfide isomerase involved in several diseases related to oxidative stress, energy metabolism and cellular inflammation. In previous manuscript, negative association between fatty liver development hepatic Txndc5 expression was observed. To study the role of TXNDC5 liver, we generated Txndc5-deficient mice. The absence caused an increased metabolic need gain weight along with bigger fatter liver. RNAseq performed elucidate putative...
The type and amount of dietary protein have become a topic renewed interest, considering their involvement in several diseases. However, little attention has been devoted to the effect avian proteins despite wide human consumption. In previous study, we saw that compared with soybean protein, consumption proteins, depending on sex, resulted similar or lower atherosclerosis higher paraoxonase 1 activity, an antioxidant enzyme carried by high-density lipoproteins (HDL). This suggests under...