A5063857725- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Glycosylation and Glycoproteins Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Nerve injury and regeneration
- Reproductive System and Pregnancy
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Axon Guidance and Neuronal Signaling
- Toxin Mechanisms and Immunotoxins
Karolinska Institutet
2021-2025
Rockefeller University
2023
Fujian University of Traditional Chinese Medicine
2017
The hallmark autoantibodies in rheumatoid arthritis are characterized by variable domain glycans (VDGs). Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development. However, functional consequences VDGs on autoreactive B cells remain elusive. Combining crystallography, glycobiology, cell assays allowed us to dissect key characteristics human biology. Crystal...
Abstract Although elevated levels of anti-citrullinated protein antibodies (ACPAs) are a hallmark rheumatoid arthritis (RA), the in vivo functions these remain unclear. Here, we have expressed monoclonal ACPAs derived from patients with RA, and analyzed their mice, as well specificities. None showed arthritogenicity nor induced pain-associated behavior mice. However, one antibodies, clone E4, protected mice antibody-induced arthritis. E4 binding pattern restricted to skin, macrophages...
To determine anticitrullinated protein antibody (ACPA) responses to novel peptides predicting the clinical outcomes of treatment-naïve early rheumatoid arthritis (RA) in presymptomatic stage. We analysed monoclonal ACPAs derived from RA patients, including a characterised protective ACPA (clone E4), along with plasma samples collected 520 individuals, whom 244 were also sampled at diagnosis RA, and 530 population controls Sweden. The validation cohort (The Nordic Rheumatic Diseases Strategy...
B cells undergo several rounds of selection to eliminate potentially pathogenic autoreactive clones, but in contrast T cells, evidence positive remains moot. Using unique tetramers, we traced natural (C1-B) specific for a defined triple-helical epitope on collagen type-II (COL2), constituting sizeable fraction the physiological cell repertoire mice, rats, and humans. Adoptive transfer C1-B suppressed arthritis independently IL10, separating them from IL10-secreting regulatory cells....
Abstract Complex autoimmune diseases are sexually dimorphic. An interplay between predisposing genetics and sex-related factors probably controls the sex discrepancy in immune response, but underlying mechanisms unclear. Here we positionally identify a polymorphic estrogen receptor binding site that regulates Cd2 expression, leading to female-specific differences T cell-dependent mouse models of autoimmunity. Female mice with reduced expression have impaired autoreactive cell responses....
Gualou Guizhi decoction (GLGZD) is effective for the clinical treatment of limb spasms caused by ischemic stroke, but its underlying mechanism unclear. Propidium iodide (PI) fluorescence staining, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), immunohistochemistry, western blot, and real-time qPCR were used to observe axonal regeneration neuroprotective effects GLGZD aqueous extract on organotypic cortical slices exposed oxygen-glucose deprivation (OGD)...
Animal models for complex diseases are needed to position and analyze the function of interacting genes. Previous positional cloning identified Ncf1 Clec4b be major regulators arthritis in rats. Here, we investigate epistasis between Clec4b, two We find that exert an additive effect on given by their joint ability regulate neutrophils. Both genes highly expressed neutrophils, together regulating neutrophil availability capacity generate reactive oxygen species. Using a glycan array, identify...
Abstract Complex autoimmune diseases are sexually dimorphic. An interplay between predisposing genetics and sex-related factors likely determines the sex discrepancy in immune response, but conclusive evidence is lacking regarding underlying molecular mechanisms. Using forward genetics, we positionally identified a polymorphic estrogen receptor binding site that regulates CD2 expression, leading to female-specific differences mouse models of T cell-dependent autoimmunity. Female mice with...