A5078546544- Renal Transplantation Outcomes and Treatments
- Pharmacological Effects and Toxicity Studies
- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Gastrointestinal motility and disorders
- Inhalation and Respiratory Drug Delivery
- Analytical Chemistry and Chromatography
- Immunotherapy and Immune Responses
- Neurological Complications and Syndromes
- Traditional and Medicinal Uses of Annonaceae
- Oxidative Organic Chemistry Reactions
- Systemic Lupus Erythematosus Research
- Nanoparticle-Based Drug Delivery
- Liver Disease Diagnosis and Treatment
- Antibiotics Pharmacokinetics and Efficacy
- Estrogen and related hormone effects
- HIV/AIDS drug development and treatment
- Gastroesophageal reflux and treatments
- Porphyrin and Phthalocyanine Chemistry
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Analytical Methods in Pharmaceuticals
- Medical Imaging and Pathology Studies
- Synthetic Organic Chemistry Methods
Haradoi Hospital
2014-2024
Hokkaido Pharmaceutical University
2013-2023
Hokkaido University of Science
2019-2023
Hokkaido University
2022
Ohu University
2007-2014
Akita University Hospital
1999-2010
Akita University
1996-2008
Niigata University
2006
Duke Medical Center
2001-2003
Hyogo Medical University
2002-2003
Background. A body-weight-based dose of tacrolimus often results in marked individual diversity blood drug concentration. Tacrolimus is a substrate for cytochrome P450 (CYP) 3A5 and p-glycoprotein encoded by CYP3A5 MDR1 (ABCB1), respectively, having multiple single nucleotide polymorphisms. In this study, we genotyped A6986G, G2677(A/T), C3435T polymorphisms investigated the association between these pharmacokinetics renal transplant recipients. Methods. Thirty consecutive recipients were...
Abstract A low concentration (10 nM) of adenosine potentiated hippocampal neuronal activity via 2a receptors without affecting presynaptic glutamate release or postsynaptic glutamatergic conductance. Adenosine inhibited uptake through the glial transporter, GLT‐1, receptors. In addition, stimulated GLT‐1‐independent from astrocytes, possibly in response to a rise intracellular Ca 2+ , involving PKA activation. Those actions could lead an increase synaptic concentrations responsible for...
The present study investigated the incidence of posttransplant diabetes mellitus (PTDM) and calculated risk developing PTDM under a tacrolimus-based immunosuppression based on clinical characteristics, tacrolimus pharmacokinetics, genetic polymorphisms related to pharmacokinetics or mellitus.Seventy nondiabetic adult kidney recipients were studied. Patients with continuous high plasma glucose levels, over 6.5 mg/dl hemoglobin A1c, requiring insulin and/or oral antidiabetic agents for more...
To elucidate the role of NK1.1+ T cells in antitumor immune response, we established cloned cell lines from tumor-infiltrating lymphocytes (TIL) B16 melanoma, and examined their mode action generating effector both vitro vivo. An clone (TM4.2) was phenotypically CD3+ TCR-alphabeta+ CD4- CD8- NK1.1+, CD28+. The TM4.2 suppressed generation anti-B16 melanoma CTLs, but not function CTLs. results using a transwell membrane suggested that suppressive activity mediated by soluble factors direct to...
It has been shown that centrally administered neuropeptide Y (NPY) delays gastric emptying. To determine the receptor subtypes of NPY mediating inhibitory effects on emptying, intracerebroventricular injection NPY, [Leu 31 ,Pro 34 ]NPY (a 1 agonist) and NPY-(3–36) 2 solid emptying postprandial antropyloric motility were studied in conscious rats. Intracerebroventricular NPY-(3–36), but not ] delayed a dose-dependent manner (0.03–3 nmol). After feeding (40 min), contractions with low...
We investigated the mycophenolic acid (MPA) chronopharmacokinetics and relation between MPA circadian exposure incidence of acute rejection (AR). The association selected genetic polymorphisms clinical events or was also studied.Thirty recipients were studied one month after renal transplantation. Mycophenolate mofetil (MMF) administered twice a day at single dose 0.5 g in four patients, 0.75 eight 1 18 patients.The daytime area under concentration-time curve (AUC0-12) larger than nighttime...
The circadian variation of clinical pharmacokinetics tacrolimus was studied using 16 adult renal transplant recipients 1 month after the operation. were administered twice a day (9 a.m. and 9 p.m.), whole‐blood samples obtained just prior to 1, 2, 3, 6, 9, 12 hours oral administration. Histological specimens kidney collected by an allograft core biopsy on 28 transplantation. There no changes in area under concentration‐time curve (AUC 0–12 ) (214 ng•h/mL during daytime vs. 223 nighttime)...
Zein microspheres conjugated with antitumor drugs (mitomycinc (MMC), daunomycin hydrochloride (DM), peplomycin sulfate (PEP)) were prepared by using a dimethyl sulfoxide (DMSO)-H2O system. MMC low solubility in H2O was easily entrapped the standard procedure, whereas some modifications required for moderately and highly soluble such as DM PEP.Colorimetric determination of achieved use phenol-sulfuric acid method sugar moieties their molecules, PEP, while simple treatment concentrated...
WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT • Circadian variations of tacrolimus pharmacokinetics are controversial. Also, the has time‐dependent variability, such as a decrease in oral clearance and increase dose‐adjusted AUC after transplantation. Although CYP3A5 polymorphism is associated with pharmacokinetics, differences influence this gene on between early maintenance stages have not yet been clarified. STUDY ADDS Tacrolimus did show circadian variation either or stage our designated‐time...
1 The pylorus plays an important role in the regulation of gastric emptying. In addition to autonomic neuropathy associated with long-standing diabetes, acute hyperglycaemia per se has effects on this study, central nervous system modulating distension-induced pyloric relaxation was investigated. 2 Gastric significantly reduced by subdiaphragmatic vagotomy, hexamethonium (20 mg kg−1) and NG-nitro-L-arginine methyl ester (L-NAME; 10 kg−1), a nitric oxide synthase (NOS) biosynthesis inhibitor,...
Rabeprazole is metabolized to some extent by CYP2C19. The purpose of this study was elucidate the pharmacokinetics each rabeprazole enantiomer in three different CYP2C19 genotype groups.Twenty-four healthy subjects, whom were homozygous extensive metabolizers (homEMs), heterozygous (hetEMs) and poor (PMs) for CYP2C19, participated our study. After a single oral dose 20 mg racemic rabeprazole, plasma concentrations enantiomers measured over course 24 h.The area under concentration-time curves...
Abstract The objective of this study was to evaluate whether genetic polymorphisms CYP2C19, CYP3A5 and MDR1 significantly impact the interaction between tacrolimus rabeprazole or lansoprazole. Seventy‐three recipients were randomly assigned after renal transplantation receive repeated doses for 28 days with a regimen either 20 mg 30 Blood concentrations measured by microparticle enzyme immunoassay. mean daily dose dose‐adjusted area under plasma concentration‐time curves from 0 12 h ( AUC...
Pirfenidone (PFD) is the first and only clinically used antifibrotic drug for treatment of idiopathic pulmonary fibrosis (IPF). This study evaluated effects two metabolites PFD, 5-hydroxypirfenidone (PFD-OH) 5-carboxypirfenidone (PFD-COOH), on WI-38 cells in an vitro lung fibroblast model. The inhibitory PFD-OH PFD-COOH transforming growth factor-β1 (TGF-β1)-induced collagen synthesis were by measuring intracellular hydroxyproline, a major component protein collagen. at 300 1000 µM...