A5046101335- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Light effects on plants
- Plant biochemistry and biosynthesis
- Chromosomal and Genetic Variations
- Nematode management and characterization studies
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Plant Disease Management Techniques
- Plant Stress Responses and Tolerance
- Plant and animal studies
- Plant Pathogens and Fungal Diseases
- Wheat and Barley Genetics and Pathology
- Potato Plant Research
- Plant Physiology and Cultivation Studies
- CRISPR and Genetic Engineering
- Horticultural and Viticultural Research
- Rice Cultivation and Yield Improvement
- Soybean genetics and cultivation
- Cassava research and cyanide
- Greenhouse Technology and Climate Control
- Plant Disease Resistance and Genetics
- Crop Yield and Soil Fertility
Palacký University Olomouc
2015-2024
Centre of the Region Haná for Biotechnical and Agricultural Research
2022-2023
Czech Academy of Sciences, Biology Centre
2015-2020
University Hospital Olomouc
2015
Czech Academy of Sciences, Institute of Experimental Botany
2012-2014
Université d'Angers
2008
Laboratoire de Biotechnologies Végétales Appliquées aux Plantes Aromatiques et Médicinales
2004-2007
Université Jean Monnet
2007
Université Paris-Sud
2007
Institut Galien Paris-Saclay
2007
Barley is one of the most important cereals, which used for breweries, animal and human feeds. Genetic manipulation plant hormone cytokinin (CK) levels may influence several physiological processes, besides others stress tolerance, root formation crop yield. In planta, endogenous CK status finely regulated by enzyme dehydrogenase (EC 1.5.99.12; CKX), that irreversibly cleaves N6-side chain adenine-derived CKs. Increasing grain yield mean content was assayed silencing HvCKX1 gene. Moreover,...
Light controls bud burst in many plants, which subsequently affects their architecture. Nevertheless, very little is known about this photomorphogenic process. This study ascertains the effects of light on and two its components, i.e. growth preformed leaves meristem organogenesis six cultivars from three Rosa species (R. hybrida L., R. chinensis wichurana L.). Defoliated plants were severed above third basal exposed, either to darkness or different intensities white light, blue, red FR, at...
Abstract Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of biosynthetic pathway leading to phenolic methyl ether 3,5-dimethoxytoluene (DMT), major scent compound many rose (Rosa x hybrida) varieties. Modern roses are descended from both European Chinese species, latter being producers ethers but not former. Here we investigated why production occurs in some all In DMT-producing varieties, OOMTs were shown be localized specifically petal, predominanty adaxial epidermal...
It has long been known that hormones affect the interaction of a phytopathogen with its host plant. The pathogen can cause changes in plant hormone homeostasis directly by affecting biosynthesis or metabolism synthesizing and secreting itself. We previously demonstrated pathogenic fungi Fusarium species complex are able to produce three major types hormones: auxins, cytokinins, gibberellins. In this work, we explore levels these maize mango tissues infected Fusarium. ability individual...
Water availability is undoubtedly one of the most important environmental factors affecting crop production. Drought causes a gradual deprivation water in soil from top to deep layers and can occur at diverse stages plant development. Roots are first organs that perceive deficit their adaptive development contributes drought adaptation. Domestication has contributed bottleneck genetic diversity. Wild species or landraces represent pool diversity not been exploited yet breeding program. In...
Dark-induced growth (skotomorphogenesis) is primarily characterized by rapid elongation of the hypocotyl. We have studied role abscisic acid (ABA) during development young tomato (Solanum lycopersicum L.) seedlings. observed that ABA deficiency caused a reduction in hypocotyl at level cell and ABA-deficient plants could be improved treatment with exogenous ABA, through which show concentration dependent response. In addition, accumulated dark-grown seedlings grew rapidly, whereas grown under...
• Background and Aims Moss roses are old garden covered with a mossy growth on flower pedicel calyx. This moss releases pine-scented oleoresin that is very sticky odoriferous. Rosa × centifolia ‘muscosa’ was the first rose to be obtained by bud-mutation but, interestingly, R. damascena ‘Quatre Saisons Blanc Mousseux’ repeat-blooming cultivar, thus interesting breeders. In present study, anatomy of these sports (i.e. bud-mutations) characterized volatile organic compounds (VOCs) produced...
SUMMARY In cereals, the root system is mainly composed of post‐embryonic shoot‐borne roots, named crown roots. The CROWN ROOTLESS1 (CRL1) transcription factor, belonging to ASYMMETRIC LEAVES2‐LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) family, a key regulator initiation in rice ( Oryza sativa ). Here, we show that CRL1 can bind, both vitro and vivo , not only LBD‐box DNA sequence recognized by several ASL/LBD factors, but also another previously identified motif was CRL1‐box . Using...
The role of abscisic acid (ABA) during early development was investigated in tomato seedlings. endogenous content ABA particular organs analyzed seedlings grown the dark and under blue light. Our results showed that dark-grown seedlings, accumulation maximal cotyledons elongation zone hypocotyl, whereas blue-light, distinctly reduced. data are consistent with conclusion promotes growth etiolated suggest plays an inhibitory de-etiolation photomorphogenesis tomato.
We localized the tissues and cells that contribute to scent biosynthesis in scented non-scented Rosa x hybrida cultivars as part of a detailed cytological analysis rose petal. Adaxial petal epidermal have typical conical, papillate shape whereas abaxial are flat. Using two different techniques, solid/liquid phase extraction headspace collection volatiles, we showed that, roses, both layers capable producing emitting despite morphologies these tissues. Moreover, OOMT, an enzyme involved...
Mitosis and cytokinesis are fundamental processes through which somatic cells increase their numbers allow plant growth development. Here, we analyzed the organization dynamics of mitotic chromosomes, nucleoli, microtubules in living barley root primary meristems using a series newly developed stable fluorescent protein translational fusion lines time-lapse confocal microscopy. The median duration mitosis from prophase until end telophase was 65.2 78.2 min cytokinesis. We showed that...
The spontaneous mutant 7B-1 in tomato (Solanum lycopersicum=Lycopersicon esculentum) is a photoperiod-dependent male-sterile previously reported as resistant to various abiotic stresses specifically under blue light. Since this finding improved the potential of 7B-1's use breeding programmes, its susceptibility stress induced by coronatine (COR), phytotoxine produced several Pseudomonas syringae strains, was assessed study. found be less sensitive than corresponding wild type (WT) COR...
Light is one of the most important factor influencing plant growth and development all through their life cycle. One well-known light-regulated processes de-etiolation, i.e. switch from skotomorphogenesis to photomorphogenesis. The hormones cytokinins (CKs) play an role during establishment photomorphogenesis as exogenous CKs induced dark-grown seedlings. Most studies are conducted on model Arabidopsis, but no or few information available for crop species, such tomato (Solanum lycopersicum...
The diverse roles of mitogen-activated protein kinases (MAPKs, MPKs) in plant development could be efficiently revealed by reverse genetic studies. In Arabidopsis, mpk6 knockout mutants complete the life cycle; however, ~40% their embryos show defects leading to abnormal phenotypes seeds and seedlings' roots. Contrary Arabidopsis MPK6 , rice ( OsMPK6 ) is an essential gene as transfer DNA (T-DNA) insertion CRISPR/Cas9 induced loss-of-function mutations cause early embryo arrest. this study,...