A5041894061- DNA and Nucleic Acid Chemistry
- Porphyrin and Phthalocyanine Chemistry
- Advanced biosensing and bioanalysis techniques
- Metal complexes synthesis and properties
- Metal-Catalyzed Oxygenation Mechanisms
- RNA Interference and Gene Delivery
- Synthesis and Biological Evaluation
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Cancer therapeutics and mechanisms
- Synthesis and Characterization of Heterocyclic Compounds
- Synthesis and biological activity
- Phenothiazines and Benzothiazines Synthesis and Activities
- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Molecular Sensors and Ion Detection
- Trypanosoma species research and implications
- Supramolecular Self-Assembly in Materials
- HIV/AIDS drug development and treatment
- Tuberculosis Research and Epidemiology
- Quinazolinone synthesis and applications
- Biochemical and Molecular Research
- Research on Leishmaniasis Studies
- Photodynamic Therapy Research Studies
Laboratoire de Chimie de Coordination
2014-2023
Centre National de la Recherche Scientifique
2012-2022
Université de Toulouse
2011-2021
Université Toulouse III - Paul Sabatier
2006-2021
Institut National Polytechnique de Toulouse
2012-2018
Institut des Biomolécules Max Mousseron
2008
Université de Montpellier
2008
Inserm
2007
Hôpital Rangueil
2007
Institut de Pharmacologie et de Biologie Structurale
2005
Abstract This review article focuses on the molecular aspects of DNA cleavage by synthetic chemical nucleases (transition metal complexes endowed with redox properties and affinity) natural drugs (cytotoxic agents such as bleomycins or enediynes). Unlike deoxyribonucleases, which catalyze nucleophilic attack water phosphorus atom a particular phosphodiester entity, these nonhydrolytic DNA‐cleavers are able to oxidize sugar units, generally hydrogen abstraction. Examples oxidative each five...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotassium monopersulfate and a water-soluble manganese porphyrin complex, [Mn(TMPyP)](OAc)5, as an efficient reagent for the oxidative cleavage of DNAJean Bernadou, Genevieve Pratviel, Faiza Bennis, Marc Girardet, Bernard MeunierCite this: Biochemistry 1989, 28, 18, 7268–7275Publication Date (Print):September 1, 1989Publication History Published online1 May 2002Published inissue 1 September 1989https://doi.org/10.1021/bi00444a019Request reuse...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA G-Quadruplex Ligand with 10000-Fold Selectivity over Duplex DNAIsabelle M. Dixon, Frédéric Lopez, Agueda Tejera, Jean-Pierre Estève, Maria A. Blasco, Geneviève Pratviel, and Bernard MeunierView Author Information Laboratoire de Chimie Coordination, CNRS, 205 route Narbonne, 31077 Toulouse Cedex 4, France, INSERM IFR 31, Institut Louis Bugnard, Hôpital Rangueil, TSA 50032, 31059 9, Spanish National Cancer Center, Melchor Fernández Almagro 3,...
The multidomain non-structural protein 3 (Nsp3) is the largest encoded by coronavirus (CoV) genomes and several regions of this are essential for viral replication. Of note, SARS-CoV Nsp3 contains a SARS-Unique Domain (SUD), which can bind Guanine-rich non-canonical nucleic acid structures called G-quadruplexes (G4) We show herein that SARS-CoV-2 also SUD domain interacts with G4s. Indeed, interactions between proteins both DNA RNA G4s were evidenced G4 pull-down, Surface Plasmon Resonance...
Guanine bases in DNA are the most sensitive to oxidation. A lot of effort has been devoted understanding chemical modifications guanine under different oxidizing conditions, final goal being know which lesions can be expected vivo and their biological consequences. This article analyses mechanisms underlying oxidation by comparison between one- two-electron transfer processes. The oxidants used vitro give complementary answers. overview presents a choice some key intermediates predictive...
The capacity of G-quadruplex ligands to stabilize four-stranded DNA makes them able inhibit telomerase, which is involved in tumour cell proliferation. A series cationic metalloporphyrin derivatives was prepared by making variations on a meso-tetrakis(4-N-methyl-pyridiniumyl)porphyrin skeleton (TMPyP). binding properties nickel(II) and manganese(III) porphyrins were studied surface plasmon resonance, the nickel telomerase tested TRAP assay. nature metal influences kinetics (the process...
The complexes [L(5)Fe(II)Cl]BPh(4) and [L(5)Fe(II)(H(2)O)](BPh(4))(2) (L(5) = N,N,N'-tris(2-pyridylmethyl)-N'-methyl-ethane-1,2-diamine) have been isolated. Bernal et al. (Bernal, J.; J. Chem. Soc., Dalton Trans. 1995, 3667-3675) prepared this ligand the corresponding complex [L(5)Fe(II)Cl]PF(6). We obtained structural data of by X-ray diffraction. It crystallizes in orthorhombic space group P2(1)2(1)2(1) with a 17.645(7) Å, b 16.077(6) c 13.934(5) V 3953(3) Å(3), Z 4. presents Fe(II)-N bond...
InhA, the NADH-dependent enoyl-acyl carrier protein reductase from Mycobacterium tuberculosis (Mtb) is proposed main target of first-line antituberculosis drug isoniazid (INH). INH activity dependent on activation by catalase peroxidase KatG, a Mtb enzyme whose mutations are linked to clinical resistance INH. Other inhibitors InhA that do not require any preliminary known. The design such direct potent represents promising approach circumvent this mechanism. An ensemble-docking process with...
Materials with tailored surface properties are useful for diverse applications, in particular adsorption and/or covalent immobilization of various biomolecules such as nucleic acids and proteins. One the main challenges is to design a reactive chemistry stable binding on support, which addition keeps away from support reduce interference. We report here novel functionalization that based formation layer, covalently grafted glass slides onto amino-modified DNA probes were fixed. The layer...
The mechanism of oxidation guanine residues on double-stranded oligonucleotides (ODNs) by the chemical nuclease Mn-TMPyP/KHSO5 is reported. By using HPLC coupled to an electrospray mass spectrometer (ESI/MS) different oxidized ODN strands were directly analyzed, and labeling experiments in H218O allowed us propose a two-electron for engaged DNA. We found that imidazolone derivative (dIz) was formed trapping guanine-cation water molecule. Two reaction intermediates pathway formation dIz...
Oxidation of 2‘-deoxyguanosine dG with the chemical nuclease Mn-TMPyP/KHSO5 leads within one minute to a nearly quantitative amount 2-amino-5-[(2-deoxy-β-d-erythro-pentofuranosyl)amino]-4H-imidazol-4-one (dIz or imidazolone for short) (90%) in non-oxygen-dependent pathway and without formation 8-oxo-dG. This new mechanism dIz involves, as crucial first step, abstraction two electrons from guanine by high-valent porphyrin−Mn(V)O species, leading dG+ cation instead classical dG•+ radical...
Depending upon the type of DNA substrate, a manganoporphyrin/KHSO5 system as artificial nuclease preferably attacks at C1′ or C5′ atom deoxyribose moiety, whereby subsequent heating reaction mixtures leads to release 5-methylene-2-furanone (5-MF) and furfural (FUR). The ratio 5-MF/FUR directly reflects relative reactivities cleaving reagent with respect C5′. A possible for formation FUR is outlined below. = terminal phosphate residue, B nucleobase.
Cationic manganese-porphyrin complexes, free or targetted with an intercalating agent, are able to cleave DNA using oxygen atom donors like potassium monopersulfate magnesium monoperphthalate as coreactants. Detailed studies of the cleavage calf thymus DNA, before and after a heating step, show that bases 5-methylene-2-furanone main reaction products, indicating hydroxylation at 1′-carbon is target these chemical agents. These data confirm metalloporphyrin derivatives interact minor groove...
The capacity of a series new cationic nickel and manganese metalloporphyrins to bind in the minor groove DNA was evaluated by binding competition experiments with manganese(III)-bis-aqua-meso-tetrakis(4-N-methylpyridiniumyl)porphyrin, Mn-TMPyP, powerful artificial nuclease when associated KHSO5. four N-methylpyridiniumyl substituents on this porphyrin macrocycle are responsible for strong affinity AT-rich DNA. inhibition cleavage mediated Mn-TMPyP/KHSO5 various tested porphyrins correlated...
A bulky cationic cobalt(<sc>iii</sc>) porphyrin carrying two water molecules as axial ligands interacts in a π-stacking like mode with the 5′-external guanine quartet of G-quadruplex DNA.
G-quadruplexes (G4s) are four-stranded nucleic acid structures formed by the stacking of G-tetrads. Here we investigated their formation and function during HIV-1 infection. Using bioinformatics biophysics analyses first searched for evolutionary conserved G4-forming sequences in genome. We identified 10 G4s with conservation rates higher than those regulatory such as RRE TAR. then used porphyrin-based G4-binders to probe infection human cells native HIV-1. The efficiently inhibited...
The covalent attachment of a manganese-tris(methylpyridiniumyl)porphyrin entity to an antisense oligonucleotide allowed sequence-selective oxidative cleavage DNA when the metalloporphyrin was activated by potassium monopersulfate (KHSO5). We prepared several structurally modified metalloporphyrin−oligonucleotide conjugates in order find out most efficient compound for vitro cleavage. nature and length tether were modulated, (metal, ligand), different ways activation assayed. noticed that...
Selected double-stranded oligodeoxyribonucleotides have been used to probe, at the molecular level, DNA chain breakages induced by chemical nuclease mesotetrakis(4-N- methylpyridiniumyl)porphyrinatomanganeseIII pentaacetate/KHSO5. The results show that cleavage selectively occurs on two 3' sides of three contiguous A.T base pairs (an triplet). Hydroxylation 5' carbon deoxyribose targets represents initial damage sugar-phosphodiester backbone and leaves a phosphate aldehyde ends. fragments...