Large pore volumes and highly accessible mesopore surface areas are present in ordered mesoporous bioactive glasses (MBGs) prepared by a block copolymer templating approach under non-aqueous conditions. These have high bone-forming bioactivity vitro, as shown immersing them simulated body fluid (SBF) monitoring the formation of hydroxycarbonate apatite (HCA) on (see electron micrographs).

The investigation on the formation mechanism of helical structures and synthesis materials is attractive for scientists in different fields. Here we report mesoporous with chiral channels presence achiral surfactants. More importantly, suggest a simple purely interfacial interaction to explain spontaneous mesostructures. Unlike proposed model molecular chains or surpramolecular packing based geometrically motivated entropically driven model, origin mesostructured may be attributed...

Abstract The synthesis of ultrasmall, well‐dispersed, hollow siliceous spheres (HSSs) by using a block copolymer as the template and tetraethoxysilane silica source under acidic conditions is reported. After removing surfactant core as‐synthesized, spherical, silica‐coated block‐copolymer micelles, HSSs with uniform particle size 24.7 nm, cavity diameter 11.7 wall thickness 6.5 nm are obtained. It shown that surface functionalization methyl groups during synthesis, can be further dispersed...

A silver-catalyzed tandem carbon–carbon triple bond hydroazidation, radical addition, and cyclization of biphenyl acetylene is described under mild conditions, leading to the formation 6-methyl sulfonylated phenanthridines in good yields. In this novel cascade reaction, most atoms are incorporated into product without cleavage C≡C bond. Mechanistic studies suggest reaction should proceed through an iminyl reactive intermediate.

We report the synthesis and characterization of a novel mesoporous material with face-centered cubic (fcc) symmetry intrinsic bimodal pores. Moreover, an icosahedral (ICO) mesostructure both meso- macroscale 5-fold is observed. propose hard-sphere packing (HSP) mechanism for formation materials by assuming preformed robust surfactant/silicate composite micelles being hard spheres. In comparison to conventional liquid crystal templating (LCT) or cooperative self-assembly (CSA) mechanism, our...

A novel nanopore based digestion strategy has been developed by directly adding a macroporous material as catalyst to the conventional in-solution reaction system. Without increasing enzyme or protein concentrations, this simple approach exhibits high proteolysis efficiency and selectivity due in situ fast adsorption of both enzymes proteins from bulk solution into macropores catalysts, where target substrates are greatly concentrated confined nanospace realize quick digestion. Based on...

Abstract Doxorubicin is an important class of anthracycline antitumor antibiotics produced by Streptomyces peucetius . The doxorubicin fermentation yield the wild-type strain was very low, so it could not be directly at industrial scale due to high cost. In present study, S. SIPI-7-14 obtained from SIPI-14 through several rounds resistance screening. Then, ketoreductase gene dnrU knocked out reduce (13 S )-13-dihydrodaunorubicin production, and drrC overexpressed further enhance doxorubicin....

Abstract A new type of gold catalyzed intermolecular dearomative cyclization reaction between diazo oxindole and tryptamine has been developed by utilizing the combination L 3 AuCl/Ag PO 4 ( =hexamethyl phosphane triamine) as catalyst. This method allows for facile preparation a series C‐3‐oxindole fused pyrroloindoline products in moderate to good yields with high diastereoselectivities. plausible mechanism, involving cascade process regioselective nucleophilic addition carbophilic...

(S)-2-Chloro-1-(3,4-difluorophenyl)ethanol (1) is a vital chiral intermediate for the synthesis of Ticagrelor—an effective treatment acute coronary syndromes. A ketoreductase (KRED) KR-01 in our KRED library was screened to transform 2-chloro-1-(3,4-difluorophenyl)ethanone (2) into alcohol 1. During process optimization, bioreduction procedure performed at substrate concentration 500 g/L, giving near 100% conversion with >99.9% ee. The product 1 directly obtained by extraction and can be...

Ergothioneine (ERG) is an unusual sulfur-containing amino acid with antioxidant activity that can be synthesized by certain bacteria and fungi. Microbial fermentation a promising method for ERG production. In this study, the bifunctional enzyme methyltransferase-sulfoxide synthase NcEgt1 from Neurospora crassa was truncated to obtain sulfoxide TNcEgt1, which showed higher expression level in Escherichia coli BL21(DE3). Then, genes egtD encoding methyltransferase EgtD egtE C-S lyase EgtE...

Große Porenvolumina und sehr gut zugängliche Porenoberflächen kennzeichnen die hochgeordneten mesoporösen bioaktiven Gläser (MBGs), in nichtwässrigen Systemen mithilfe eines Blockcopolymer-Templats hergestellt wurden. Die hohe In-vitro-Knochenbildungsaktivität dieser wird durch ihr Eintauchen simulierte Körperflüssigkeit (SBF) das Verfolgen der Bildung von Hydroxy(carbonat)apatit (HCA) auf Oberfläche belegt (siehe Elektronenmikrographen). Supporting information for this article is available...

A p6m to Ia3d mesophase evolution is achieved by simply adjusting the acidity and/or anion species in presence of block copolymers; unusual sequence that affects phase behavior copolymer templated mesostructured solids revealed be SO(4)(2-)(HSO(4)(-)) > NO(3)(-) Br(-) Cl(-).

A chemoenzymatic synthesis of the veterinary antibiotic florfenicol is described. The key step involves dynamic reductive kinetic resolution (DYRKR) a keto ester by using ketoreductase‐02 (KRED‐02) to afford two contiguous stereocenters (2 S ,3 R )‐ cis ‐1,2‐amino alcohol intermediate in >99 % ee and diastereomeric ratio ( dr ) 99 %. This green biocatalysis environmental friendly with high enantioselectivity product yields. Two methods for nucleophilic fluorination involved use aziridines...

Chiral vicinal amino alcohols were generated with excellent stereoselectivity and high conversion from bulky α-amino β-keto esters by an engineered ketoreductase called M30.

Label Distribution Learning (LDL) is a novel machine learning paradigm that assigns label distribution to each instance. Numerous LDL methods proposed leverage correlation in the process solve exponential-sized output space; among these, many exploited low-rank structure of capture correlation. However, recent research has unveiled matrices typically maintain full rank, posing challenge approaches relying on Notably, finds widespread adoption multi-label (MLL) literature due often nature...

(S)-2-Chloro-1-(2,4-dichlorophenyl) ethanol (3) is a chiral intermediate in the synthesis of luliconazole ((R)-E-1). Here, we report novel biopreparation 3 by bioreduction 2-chloro-1-(2,4-dichlorophenyl) ethanone (2) using recombinant Escherichia coli expressing LK08, ketoreductase mutant from Lactobacillus kefiri, as biocatalyst. The reaction conditions for biotransformation including pH, temperature, and concentration isopropanol NADP+, well amount E. cells, were optimized to improve...

A novel two-fold interpenetrated metal-organic framework, namely Co-EDDA, was synthesized by hydrothermal reaction of 5,5'-[ethane-1,2-diylbis(oxy)]diisophthalic acid (H4EDDA), Co(NO3)2·6H2O, and 1,4-di(1H-imidazol-1-yl)benzene in water an alkaline environment structurally characterized. Co-EDDA could display clear dual-emission signals at 350 430 nm, representing the charge transfer emission between metal ions ligand ligand-based emission, respectively, which represents ratiometric...

In the present study, a practical chemoenzymatic method was developed for synthesis of chiral phenylalanine derivative 1, which is key intermediate Lifitegrast. this established process, simple and effective Zn/HCl reduction system employed to obtain N-acetyl-3-bromo-phenylalanine 6, served as substrate subsequent enzymatic resolution process. Then, intermediate, 3-bromo-l-phenylalanine 7, could be obtained using acylase (ACY) AmACY. Upon process optimization, chemical reaction executed at...