A5053032683- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Virus-based gene therapy research
- Electrospun Nanofibers in Biomedical Applications
- Advanced Drug Delivery Systems
- MicroRNA in disease regulation
- Pancreatic function and diabetes
- Drug Solubulity and Delivery Systems
- Drug Transport and Resistance Mechanisms
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- Analytical Chemistry and Chromatography
- Lipid Membrane Structure and Behavior
- Advanced Cellulose Research Studies
- Polymer Surface Interaction Studies
- Biochemical effects in animals
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Pluripotent Stem Cells Research
- DNA and Nucleic Acid Chemistry
- Hydrogels: synthesis, properties, applications
- Cancer Research and Treatments
University of Helsinki
2015-2024
University of Padua
2016-2020
Data Harbor (United States)
2015
Inserm
2012
Hôtel-Dieu de Paris
2012
Islamia University of Bahawalpur
2011-2012
Université Paris Cité
2011
Délégation Paris 5
2011
Tampere University
2008
Harvard–MIT Division of Health Sciences and Technology
2008
Extracellular vesicles (EVs) are naturally occurring membrane particles that mediate intercellular communication by delivering molecular information between cells. In this study, we investigated the effectiveness of two different populations EVs (microvesicle- and exosome-enriched) as carriers Paclitaxel to autologous prostate cancer cells.EVs were isolated from LNCaP- PC-3 cell cultures using differential centrifugation characterized electron microscopy, nanoparticle tracking analysis,...
Over the recent years, various materials have been introduced as potential 3D cell culture scaffolds. These include protein extracts, peptide amphiphiles, and synthetic polymers. Hydrogel scaffolds without human or animal borne components added bioactive are preferred from immunological point of view. Here we demonstrate that native nanofibrillar cellulose (NFC) hydrogels derived abundant plant sources provide desired functionalities. We show 1) rheological properties allow formation a...
Current analysis of exosomes focuses primarily on bulk analysis, where exosome‐to‐exosome variability cannot be assessed. In this study, we used Raman spectroscopy to study the chemical composition single exosomes. We measured spectra individual from 8 cell lines. Cell‐line‐averaged varied considerably, reflecting variation in total exosomal protein, lipid, genetic, and cytosolic content. Unexpectedly, isolated same type also exhibited high spectral variability. Subsequent revealed...
BACKGROUND Extracellular vesicles (EVs) are cell-derived membrane vesicles. EVs contain several RNAs such as mRNA, microRNAs, and ncRNAs, but less is known of their genomic DNA (gDNA) content. It also unknown whether the cargo randomly sorted or if it systematically packed into specific EV subpopulations. The aim this study was to analyze different prostate cancer (PCa) subpopulations (apoptotic bodies, microvesicles, exosomes) carry gDNA fragments. METHODS were isolated from three PCa cell...
Although new therapeutic approaches for burn treatment have made progress, there is still need better methods to enhance wound healing and recovery especially in severely burned patients. Nanofibrillar cellulose (NFC) has gained attention due its renewable nature, good biocompatibility excellent physical properties that are of importance a range applications pharmaceutical biomedical fields. In the present study, we investigated potential wood based NFC dressing clinical trial on Previously,...
Body fluids are a rich source of extracellular vesicles (EVs), which carry cargo derived from the secreting cells. So far, biomarkers for pathological conditions have been mainly searched their protein, (mi)RNA, DNA and lipid cargo. Here, we explored small molecule metabolites urinary platelet EVs relative to matched samples. As proof-of-concept study intra-EV metabolites, compared alternative normalization methods profile prostate cancer patients before after prostatectomy healthy...
Physiologically relevant hepatic cell culture models must be based on three-dimensional (3D) of human cells. However, liver cells are generally cultured in two-dimensional (2D) format that deviates from the normal vivo morphology. We generated 3D environment for HepaRG progenitor using wood-derived nanofibrillar cellulose (NFC) and hyaluronan-gelatin (HG) hydrogels. Culture undifferentiated NFC HG hydrogels induced formation multicellular spheroids with apicobasal polarity functional bile...
Human embryonic stem cells and induced pluripotent have great potential in research therapies. The current vitro culture systems for human (hPSCs) do not mimic the three-dimensional (3D) vivo cell niche that transiently supports proliferation is subject to changes which facilitate subsequent differentiation during development. Here, we demonstrate, first time, a novel plant-derived nanofibrillar cellulose (NFC) hydrogel creates flexible 3D environment hPSC culture. pluripotency of hPSCs...
One of the greatest bottlenecks in extracellular vesicle (EV) research is production sufficient material a consistent and effective way using vitro cell models. Although EVs bioreactors maximizes EV yield comparison to conventional cultures, impact their growth conditions on has not yet been established. In this study, we grew two prostate cancer lines, PC-3 VCaP, culture dishes two-chamber elucidate how environment affects characteristics. Specifically, wanted investigate...
Standard of care for cancer is commonly a combination surgery with radiotherapy or chemoradiotherapy. However, in some advanced patients this approach might still remaininefficient and may cause many side effects, including severe complications even death. Oncolytic viruses exhibit different anti-cancer mechanisms compared conventional therapies, allowing the possibility improved effect therapy. Chemotherapeutics combined oncolytic stronger cytotoxic responses oncolysis. Here, we have...
Abstract Cell surface p32, the target of LyP‐1 homing peptide, is upregulated in tumors and atherosclerotic plaques has been widely used as a receptor for systemic delivery payloads. Here, we identified an improved LyP‐1‐mimicking peptide (TT1, CKRGARSTC). We this fluorescence polarization‐based high‐throughput screening 50 000‐compound chemical library panel compounds that bind p32 with low micromolar affinity. Among hits screen, two were shown to specifically multiple assays. One these was...
Platelet-rich plasma (PRP) is a source of growth factors, which are implicated in active tissue regeneration. However, after transplantation the efficacy these bioactive compounds often diminished due to rapid degradation and untargeted localization. For this reason, we evaluated potential nanofibrillated cellulose (NFC) hydrogel as PRP carrier. NFC an animal-free biomaterial that, when doped with cellulase, can assist release wound site. In study, examined effects 0.5% (m/v) formulations,...
We have combined Langmuir monolayer film experiments and all-atom molecular dynamics (MD) simulation of a bilayer to study the surface structure PEGylated liposome its interaction with ionic environment present under physiological conditions. Lipids that form both gel liquid-crystalline membranes been used in our study. By varying salt concentration experiment including at level simulation, we studied effect ions blood plasma on poly(ethylene glycol) (PEG) layer. also between PEG layer lipid...