A5036659517- Acute Myeloid Leukemia Research
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- Electrolyte and hormonal disorders
- Acute Lymphoblastic Leukemia research
- Hormonal Regulation and Hypertension
- Ion Transport and Channel Regulation
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Hematopoietic Stem Cell Transplantation
- Chronic Lymphocytic Leukemia Research
- Hematological disorders and diagnostics
- Cutaneous lymphoproliferative disorders research
- Pharmacological Effects and Assays
- Single-cell and spatial transcriptomics
- Blood disorders and treatments
- Social and Educational Sciences
- Reproductive Biology and Fertility
- Advanced Biosensing Techniques and Applications
- Silk-based biomaterials and applications
- Blood groups and transfusion
University Hospitals Bristol NHS Foundation Trust
2011-2025
Uppsala University
2001-2023
Bristol Royal Infirmary
2013-2023
Massachusetts General Hospital
2023
Linnaeus University
2017-2023
University Hospitals Bristol and Weston NHS Foundation Trust
2020-2022
Swedish University of Agricultural Sciences
2011-2022
John Wiley & Sons (United States)
2021
AstraZeneca (Sweden)
2016-2020
KTH Royal Institute of Technology
2016-2019
Sodium-glucose cotransporter 2 (SGLT2) inhibitors are a new class of antidiabetic oral agents indicating promising effects on cardiovascular and renal end points. However, the renoprotective SGLT2 not fully understood. Also, metabolic inhibition other organ systems, such as hepatic steatosis, This study sought to address these questions by treating 18-wk-old uninephrectomized db/db mice with selective inhibitor dapagliflozin. Untreated developed progressive albuminuria, glomerular mesangial...
Abstract Tissues are built of cells integrated in an extracellular matrix (ECM) which provides a three-dimensional (3D) microfiber network with specific sites for cell anchorage. By genetic engineering, motifs from the ECM can be functionally fused to recombinant silk proteins. Such protein, FN-silk, harbours motif fibronectin, has ability self-assemble into networks microfibers under physiological-like conditions. Herein we describe method by mammalian added solution before assembly, and...
Current recommendations for diagnosing myelodysplastic syndromes endorse flow cytometry as an informative tool. Most protocols focus on the analysis of progenitor cells and evaluation maturing myelomonocytic lineage. However, one most frequently observed features is anemia, which may be associated with dyserythropoiesis. Therefore, changes in nucleated erythroid complement current tools. The multicenter study within IMDSFlow Working Group, reported herein, focused defining parameters that...
Mesenchymal stem cells (MSCs) contribute to endothelial cell (EC) migration by producing proteases, thereby paving the way into tissues for ECs. MSCs were added our previously described composite EC islets as a potential means improve their capacity islet angiogenesis.Human coated with primary human bone marrow-derived and dermal microvascular The of ECs, or without MSCs, adhere grow was analyzed. survival functionality these evaluated in dynamic perifusion assay, angiogenesis vitro assessed...
The affinity for the glucocorticoid receptor in rat skeletal muscle of some glucocorticoids with a new type 16 alpha, 17 alpha-acetal substituent has been estimated and correlated to activities three vivo systems rats. Budesonide (an approximately 1:1 mixture C(22) epimers 11 beta, 21-dihydroxy-16 alpha-[(22R,S)-propylmethylenedioxy]-pregna-1,4-diene-3,20-dione) isolated (22R)- (22S)-epimers bound same binding site as potent dexamethasone (DEX) or triamcinolone alpha-acetonide (TA), but even...
Minimal residual disease detection, used for clinical management of children with acute lymphoblastic leukemia, can be performed by molecular analysis antigen-receptor gene rearrangements or flow cytometric aberrant immunophenotypes. For minimal to incorporated into larger national and international trials, a quality assured, standardized method is needed which in multi-center setting. We report four color, protocol established validated the UK leukemia Flow group. Quality assurance testing...
Please refer to Appendix I for classification of evidence levels. Standard operating procedures all areas the laboratory require validation. These and controlled documents may be audited; in particular: The guideline group comprised representatives UK-based clinical flow cytometry (FC) experts representatives. writing produced draft guidelines, which were subsequently revised by consensus members Haemato-oncology Task Force British Committee Standards Haematology (BCSH). guidelines then...
Protease-activated receptor-2 (PAR-2) belongs to a family of G-coupled receptors activated by proteolytic cleavage reveal tethered ligand. PAR-2 is trypsin and trypsin-like serine proteases experimentally, receptor-activating peptides (APs), which mimic the has recently been implicated in proinflammatory immune responses. For example, PAR-2(-/-) mice exhibit markedly diminished contact hypersensitivity reactions are completely resistant adjuvant-induced arthritis. The present study shows...
Excess mineralocorticoid receptor (MR) activation promotes target organ dysfunction, vascular injury and fibrosis. MR antagonists like eplerenone are used for treating heart failure, but their use is limited due to the compound class-inherent hyperkalemia risk. Here we present evidence that AZD9977, a first-in-class modulator shows cardio-renal protection despite mechanism-based reduced liability cause hyperkalemia. AZD9977 in vitro potency binding mode were characterized using reporter...
Abstract A peripheral blood flow cytometric assay for Sézary syndrome (SS) or circulating mycosis fungoides (MF) cells must be able to reliably identify, characterize, and enumerate T‐cells with an immunophenotype that differs from non‐neoplastic T‐cells. Although it is also important distinguish SS MF other subtypes of T‐cell neoplasm, this usually requires information in addition the immunophenotype, such as clinical morphologic features. This article outlines approach recommended by...
Abstract Background Myelodysplastic syndromes (MDS) represent a diagnostic challenge. This prospective multicenter study was conducted to evaluate pre‐defined flow cytometric markers in the work‐up of MDS and chronic myelomonocytic leukemia (CMML). Methods Thousand six hundred eighty‐two patients with suspected MDS/CMML were analyzed by both cytomorphology according WHO 2016 criteria cytometry ELN recommendations. Flow readout categorized ‘non‐MDS’ (i.e. no signs limited MDS/CMML) ‘in...
In pancreatic islet transplantation, early revascularization is necessary for long-term graft function. We have shown in vitro and vivo models that modification with surface-attached heparin protects the islets from acute attack by innate immune system of blood following intraportal transplantation. this study, we investigated ability an immobilized conjugate composed to bind angiogenic growth factor vascular endothelial factor-A (VEGF-A) as a means attracting cells (ECs) induce angiogenesis...
Transplantation of pancreatic islets is one approach for treatment diabetes, however, hampered by the low availability viable islets. Islet isolation leads to disruption environment surrounding endocrine cells, which contributes eventual cell death. The reestablishment this vital, why we herein investigated possibility using recombinant spider silk support in vitro after isolation. protein 4RepCT was formulated into three different formats; 2D-film, fiber mesh and 3D-foam, order provide a...
Abstract Background Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over past few years, significant progress has been made in FCM field concerning technical issues (including software hardware) pre‐analytical procedures. Methods Recommendations are based on data expert discussions generated from 13 yearly meetings European LeukemiaNet international MDS working group. Results We report here...
This article discusses the rationale for inclusion of flow cytometry (FCM) in diagnostic investigation and evaluation cytopenias uncertain origin suspected myelodysplastic syndromes (MDS) by European LeukemiaNet international MDS Flow Working Group (ELN iMDS WG). The WHO 2016 classification recognizes that FCM contributes to diagnosis may be useful prognostication, prediction, response therapy follow-up patients.