A5081914321- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- Nanoparticle-Based Drug Delivery
- Pharmacogenetics and Drug Metabolism
- Luminescence and Fluorescent Materials
- Nanocluster Synthesis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Crystallization and Solubility Studies
- Lanthanide and Transition Metal Complexes
- Analytical Chemistry and Chromatography
- X-ray Diffraction in Crystallography
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
University of Illinois Urbana-Champaign
2013-2017
Urbana University
2017
Goodwin College
2015
Texas A&M University
2011
Cytochrome P450 3A4 (CYP3A4) is the most abundant membrane-associated isoform of family in humans and responsible for biotransformation more than 50% drugs metabolized body. Despite large number crystallographic structures available CYP3A4, no structural information its membrane-bound state at an atomic level available. In order to characterize binding, depth insertion, membrane orientation, lipid interactions we have employed a combined experimental simulation approach this study. Taking...
A few parts per billion are enough to turn on a novel fluorescent cyanide sensor in aqueous solutions. Because of its inherent electron deficiency, the cationic boron moiety this not only serves as high-affinity anion binding site, but also quenches fluorescence pendant fluorophores (see figure; PET=photo-induced transfer). Anion center neutralizes electron-accepting properties phosphonium borane unit, leading revival fluorophore.
Membrane proteins reconstituted into phospholipid nanodiscs comprise a soluble entity accessible to solution small-angle X-ray scattering (SAXS) studies. It is demonstrated that using SAXS data it possible determine both the shape and localization of membrane protein cytochrome P450 3A4 (CYP3A4) while embedded in bilayer nanodisc. In order accomplish this, hybrid approach analysis was developed which combines an analytical describe multi-contrast nanodisc with free-form bead-model...
Nanodiscs are monodisperse, self-assembled discoidal particles that consist of a lipid bilayer encircled by membrane scaffold proteins (MSP). have been used to solubilize for structural and functional studies deliver therapeutic phospholipids. Herein, we report on tetramethylrhodamine (TMR) tagged nanodiscs lipophilic MR contrast agents generation multimodal nanoparticles cellular imaging. We incorporate both multimeric monomeric Gd(III)-based into show containing the agent (ND2) label cells...
A microfluidic platform for Nanodisc formation and membrane protein incorporation will enable studies of interactions at model interfaces.