A5061783177- CRISPR and Genetic Engineering
- Genomics and Rare Diseases
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Bacterial Genetics and Biotechnology
- Interpreting and Communication in Healthcare
- Ethics in Clinical Research
- Health Systems, Economic Evaluations, Quality of Life
- Innovative Human-Technology Interaction
- Animal Genetics and Reproduction
- Genetic Associations and Epidemiology
- Bacterial biofilms and quorum sensing
- Anesthesia and Pain Management
- Library Science and Information Literacy
- Thermal Radiation and Cooling Technologies
- Interactive and Immersive Displays
- Pluripotent Stem Cells Research
- Urban Heat Island Mitigation
- Greenhouse Technology and Climate Control
- Doctoral Education Challenges and Solutions
- Aeroelasticity and Vibration Control
- Plasma and Flow Control in Aerodynamics
Arizona State University
2017-2024
Children's Mercy Hospital
2024
Legacy Emanuel Medical Center
2022
University of Liverpool
2022
Alder Hey Children's Hospital
2022
University of Utah
2020
University of Minnesota
2014
Our work links hybrid practices from biology, fine arts, and design in a studio setting to support materially-oriented engagement with biotechnology. Using autoethnographic methods, we present our two-year process of converting an HCI into BSL-1 (biosafety level 1) facility, iterative development low-cost tools, own self-reflexive experimentation (DIY)bio protocols. Insights this led us weeklong bioart course, whereby junior highschool students creatively "painted" bacteria antibiotic...
This paper is centered on a new form of adaptive material which functions much in the same way as skeletal muscle tissue, structurally modeled plant actuator cells and capable rapidly expanding or shrinking by an order magnitude prescribed directions. Rapid changes cell shape sizes are often initiated via ion-transport driven fluid migration resulting turgor pressure variation. Certain cellular structures like those Mimosa pudica (sensitive plant), Albizia julibrissin (Mimosa tree), Dionaea...
This paper focuses on manual screenprinting as a DIY fabrication technique for embedding interactive behavior onto rage of substrates such paper, fabric, plastic, wood, or vinyl. We frame process that operates at the intersection art, technology, and material science iteratively examine its potential in two STEAM contexts. conducted youth adult workshops whereby participants worked with our low-cost thermochromic, UV-sensitive, conductive inks to develop range concepts final projects. Our...
Quorum sensing networks have been identified in over one hundred bacterial species to date. A subset of these regulate group behaviors, such as bioluminescence, virulence, and biofilm formation, by sending receiving small molecules called homoserine lactones (HSLs). Bioengineers incorporated quorum pathways into genetic circuits connect logical operations. However, the development higher-order circuitry is inhibited crosstalk, which network responds HSLs produced a different network. Here,...
Chromatin is a system of nuclear proteins and nucleic acids that plays pivotal role in gene expression cell behavior therefore the subject intense study for development cancer research. Biochemistry, crystallography, reverse genetics have elucidated macromolecular interactions drive chromatin regulation. One central mechanisms recognition post-translational modifications (PTMs) on histone by family known as "readers". This knowledge has launched wave activity around rational design interact...
A persistent challenge for mammalian cell engineering is the undesirable epigenetic silencing of transgenes. Foreign DNA can be incorporated into closed chromatin before and after it has been integrated a host cell's genome. To identify elements that mitigate silencing, we tested components from c-myb NF-kB transcriptional regulation systems in transiently transfected at chromosomally transgenes PC-3 HEK 293 cells. binding sites MYB (c-myb) placed upstream minimal promoter enhanced...
The use of glucocorticoid as local anesthetic adjuvant in single-injection adductor canal block (ACB) is well-documented but its effects the presence an indwelling catheter unclear. purpose this study was to determine impacts one-time perineural injection on continuous patients undergoing total knee arthroplasty.A single center retrospective 95 unilateral arthroplasty (TKA) performed. Patients were divided into three groups based received through ACB before placement: a control group with no...
Previously, we used an inducible, transgenic polycomb chromatin system to demonstrate that closed, transcriptionally silenced reduces Cas9 editing. Here, investigated strategies enhance editing efficiency by artificially perturbing closed chromatin. We tested UNC1999, a small molecule inhibitor blocks enhancer of zeste homolog 2, enzyme maintains also DNA-binding, transiently expressed activation-associated proteins (AAPs) are known support open, active state. When cells carried...
ABSTRACT Recently we demonstrated that closed chromatin composed of Polycomb proteins and histone 3 lysine 27 trimethylation impedes CRISPR-mediated genome editing by blocking the accessibility chromosomal DNA to spCas9/sgRNA. Editing efficiencies were higher in cells where same reporter locus had not been repressed, thus presume silenced can be modified generate a Cas9-accessible state. To test this idea, exposed antagonists silencing: Gal4-p65, targeted transcriptional activator, UNC1999,...
Emerging evidence implicates common genetic variation - aggregated into polygenic scores (PGS) impacting the onset and phenotypic presentation of rare diseases. In this study, we quantified individual liability for 1,151 previously published PGS in a cohort 2,374 probands enrolled Genomic Answers Kids (GA4K) disease revealing widespread associations between phenotypes PGSs complex diseases traits, blood protein levels, brain other organ morphological measurements. We observed increased...
How many children are struggling in their day-to-day life due to having an undiagnosed rare genetic disorder? We know that next generation sequencing has revolutionized the speed of disease (RD) diagnoses and supplementation by additional technologies such as long-read genome (lrGS), offered now clinically at Children's Mercy Hospital (CMH), holds promise increase diagnostic rates beyond 50%. However, benefits these only apply RD patients who receive testing, current shortage maldistribution...
Aim/Purpose: This article explores the critical need for adaptable educational models in times of crisis, focusing on strategies to overcome infrastructural and digital inequalities exacerbated by COVID-19 pandemic. Background: By examining a case study an offline library project implemented South Sudan, this paper seeks examine impact solution low-resource crisis situations. Methodology: The authors utilize mixed-methods approach, integrating both qualitative interviews quantitative data...
Abstract Quorum sensing networks have been identified in over one hundred bacterial species to date. A subset of these regulate group behaviors, such as bioluminescence, virulence, and biofilm formation, by sending receiving small molecules called homoserine lactones (HSLs). Bioengineers incorporated quorum pathways into genetic circuits connect logical operations. However, the development higher-order circuitry is inhibited crosstalk, which network responds HSLs produced a different...
While stem cell therapies hold promise as regenerative treatments for combating a variety of conditions, the majority these remain in clinical trials without sufficient evidence current use. Predatory direct-to-consumer clinics (hereafter, DTC clinics), undeterred by lack evidence, continue to offer unapproved injections patients desperate treat chronic conditions. These can pose significant physical and financial harm their are not risks. Clinics rarely practice requisite level follow-up...