Chromosomal DNA damage can be a result of several processes and agents endogenous or exogenous origin. These cause strand breaks oxidized bases that lead to breaks, which relax the normally supercoiled genomic increase its electrophoretic mobility. The extent assessed by single cell gel electrophoresis, where chromosomal migration distance correlates with damage. This technique has been used for variety applications organisms, but only few studies have reported Saccharomyces cerevisiae. A...

Pain sensation and aversive behaviors entail the activation of nociceptor neurons, whose function is largely conserved across animals. The functional heterogeneity nociceptors ethical concerns are challenges for their study in mammalian models. Here, we investigate a single type genetically identified C. elegans thermonociceptor named FLP. Using calcium imaging vivo, demonstrate that FLP encodes thermal information tonic graded manner over wide range spanning from noxious cold to heat...

ABSTRACT The sense of touch relies on the continuous function specialized mechanosensory circuits, but underlying molecular mechanisms remain poorly understood. Here, we report that conserved transcription factors (TFs) CFI-1 (ARID3) and EGL-5 (HOXA7) jointly maintain in adult C. elegans identity two key interneuron types, securing information processing within a circuit. Toggling between normal low levels or adults generated digital-like (ON/OFF) effects both touch-evoked escape response...

AT-rich interaction domain 3 (ARID3) transcription factors are expressed in the nervous system, but their mechanisms of action largely unknown. Here, we provide, vivo, a genome-wide binding map for CFI-1, sole C. elegans ARID3 ortholog. We identify 6,396 protein-coding genes as putative direct targets most which encode neuronal terminal differentiation markers. In head sensory neurons, CFI-1 directly activates multiple genes, thereby acting selector. motor however, acts repressor,...

Understanding how the nervous system bridges sensation and behavior requires elucidation of complex neural molecular networks. Forward genetic approaches, such as screens conducted in C. elegans, have successfully identified genes required to process natural sensory stimuli. However, functional redundancy within underlying circuits, which are often organized with multiple parallel pathways, limits our ability identify 'neural pathway-specific genes', i.e. that essential for function some,...

In order to thrive in constantly changing environments, animals must adaptively respond threatening events. Noxious stimuli are not only processed according their absolute intensity, but also context. Adaptation processes can cause habituate at different rates and degrees response permanent or repeated stimuli. Here, we used a forward genetic approach Caenorhabditis elegans identify neuropeptidergic pathway, essential prevent fast habituation maintain robust withdrawal responses noxious This...

Ryanodine receptors (RyR) are essential regulators of cellular calcium homeostasis and signaling. Vertebrate genomes contain multiple RyR gene isoforms, expressed in different tissues executing functions. In contrast, invertebrate a single RyR-encoding it has long been proposed that transcripts generated by alternative splicing may diversify their Here, we analyze the expression function exons C . elegans unc-68 We show specific isoform subsets created via promoters Divergent Region 2 (DR2),...

The cell adhesion molecules NCAM and L1 are considered to play key roles in neuronal development plasticity. has been shown interact with NCAM, possibly through binding oligomannosidic glycans present L1. We investigated the effect of recombinant immunoglobulin (Ig) modules involved homophilic binding, on induced neurite outgrowth from PC12‐E2 cells found a complete inhibition after addition Ig‐modules 1, 2 3 suggesting that ligation state is crucial for normal signaling.

In this work the model organism Saccharomyces cerevisiae was used to characterise biological activity and mechanism of action plant extracts phytochemicals. One goals use mutant strains affected in oxidative stress response DNA repair order uncover molecular targets damage assessed with comet assay [1,2], using “comet tail length” as a measure damage, which displayed dose-response relationship different DNA-damaging agents. Typical experiments involved incubation yeast cells or...

The auxin-inducible degradation system has emerged as a powerful tool to deplete proteins of interest in cells and tissues various model organisms, including C. elegans 2-5. Here, we present detailed protocol perform AID-driven spatiotemporal depletion specific tissues. First, introduced the AID degron fluorescent reporter at two conserved proteins: (a) transcription factor CFI-1 (human ARID3), which is expressed nucleus multiple neurons head muscle 6,7, (b) broadly translation initiation...

Protocol for auxin-inducible protein degradation in C. elegans using different auxins and TIR1-expressing strainsThe degron (AID) system is a powerful tool to deplete proteins vivo.Here, we present protocol AID-mediated depletion of two (CFI-1/AT-rich interaction domain 3 [ARID3] Y47D3A.21/density-regulatedre-initiation release factor [DENR]) tissues transport inhibitor response 1 (TIR1)-expressing strains.We describe steps genetic crossing, sample preparation, fluorescent microscopy,...

Este relato descreve o processo de criação do time experiência usuário (UX - User Experience) da Dataprev, nos portais Auxílio Reconstrução, apoio financeiro destinado à população Rio Grande Sul, instituído pelo Ministério Integração e Desenvolvimento Regional em resposta às chuvas enchentes maio 2024. O também aborda monitoramento as lições aprendidas.

SUMMARY ARID3 transcription factors are expressed in the nervous system, but their functions and mechanisms of action largely unknown. Here, we generated vivo a genome-wide binding map for CFI-1, sole C. elegans ortholog. We identified 6,396 protein-coding genes as putative direct targets most which (77%) post-mitotic neurons encode terminal differentiation markers (e.g., neurotransmitter receptors, ion channels, neuropeptides). To gain mechanistic insights, focused on two neuron types. In...

ARID3 transcription factors are expressed in the nervous system, but their functions and mechanisms of action largely unknown. Here, we generated vivo a genome-wide binding map for CFI-1, sole C. elegans ortholog. We identified 6,396 protein-coding genes as putative direct targets most which (77%) post-mitotic neurons encode terminal differentiation markers (e.g., neurotransmitter receptors, ion channels, neuropeptides). To gain mechanistic insights, focused on two neuron types. In sensory...