A5053179147- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Insect Resistance and Genetics
- Plant Stress Responses and Tolerance
- Fungal and yeast genetics research
- Plant tissue culture and regeneration
- Nematode management and characterization studies
- Latin American rural development
- Genetics and Plant Breeding
- Insect and Pesticide Research
- Fermentation and Sensory Analysis
- Entomopathogenic Microorganisms in Pest Control
- Plant and soil sciences
- Insect Pest Control Strategies
- Plant Pathogenic Bacteria Studies
- Congenital gastrointestinal and neural anomalies
- Genomics and Phylogenetic Studies
- Natural Products and Biological Research
- Mycorrhizal Fungi and Plant Interactions
- Yeasts and Rust Fungi Studies
- Enzyme-mediated dye degradation
- Studies on Chitinases and Chitosanases
Universidad Autónoma del Estado de Morelos
2015-2025
Universidad Autónoma del Estado de México
2008-2021
Centro de Ingeniería Genética y Biotecnología
2020
Centro de Investigación de la Caña de Azúcar de Colombia
2020
Instituto Nacional de Salud Pública
2004
Universidad Nacional Autónoma de México
1995-2003
Improving stress tolerance and yield in crops are major goals for agriculture. Here, we show a new strategy to increase drought legumes by overexpressing trehalose-6-phosphate synthase the symbiotic bacterium Rhizobium etli. Phaseolus vulgaris (common beans) plants inoculated with R. etli gene had more nodules increased nitrogenase activity higher biomass compared wild-type In contrast, an mutant fewer less biomass. Three-week-old subjected fully recovered whereas or strain wilted died. The...
ABSTRACT Alfalfa ( Medicago sativa L.) is an important forage crop in many countries with high biomass production and the third‐most‐cultivated United States, protein content potential use as biofuel. Here we describe of a chimeric translational fusion yeast trehalose‐6‐phosphate synthase (EC 2.4.1.15) phosphatase 3.1.3.12) genes to engineer stress tolerance alfalfa. The gene was overexpressed using either constitutive 35S promoter or stress‐regulated rd29A promoter. Transgene insertion...
Little is known about postnatal enteric nervous system (ENS) development, but some reports suggest that the bowel may contain neural stem cells. Therefore, we created an in vitro model of desegregation using enzymatic and mechanical tissue technique. This approach yielded a group cells from small intestine lactating adult mice, which ex vivo attach to culture dish; actively proliferate; express nestin, vimentin, pro-neural transcription factors neurogenin-2 (ngn-2), Sox-10, Mash-1. In...
In this work, we report the draft genome sequence of Ensifer sp. P24N7, a symbiotic nitrogen-fixing bacterium isolated from nodules Phaseolus vulgaris var. Negro Jamapa was planted in pots that contained mining tailings Huautla, Morelos, México. The genomic DNA sequenced by an Illumina NovaSeq 6000 using 250 bp paired-end protocol obtaining 1,188,899 reads. An assembly generated with SPAdes v. 3.15.4 resulted length 7,165,722 composed 181 contigs N50 323,467 bp, coverage 76X, and GC content...
Ethanol fermentations can be prematurely halted as Saccharomyces cerevisiae faces adverse conditions, such acidic pH, presence of acetic acid, and supraoptimal temperatures. The knowledge on yeast responses to these conditions is essential endowing a tolerant phenotype another strain by targeted genetic manipulation. In this study, physiological whole-genome analyses were conducted obtain insights molecular which potentially render towards thermoacidic conditions. To end, we used...
Endophytic
In this study we cloned a chitinase gene (SmchiC), from Serratia marcescens isolated the corpse of Diatraea magnifactella lepidopteran, which is an important sugarcane pest. The SmchiC amplified S. genome was into transformation vector p2X35SChiC and used to transform tobacco (Nicotiana tabacum L. cv Petit Havana SR1). resistance these transgenic plants necrotrophic fungus Botrytis cinerea pest Spodoptera frugiperda evaluated: both activity as well against B. significantly higher in compared...
Abstract Ethylene response factors (ERFs) are a large family of plant‐specific transcription that have been implicated in positively regulating stress responses. The aim this work was to study the expression pattern ethylene SlERF1 , SlERF3b and SlERF5 Solanum lycopersicum cv. Ailsa Craig, when subjected different conditions, as well determine overexpression effect transgenic tomato exposed abiotic biotic conditions. Here we report genes differentially upregulated wild‐type plants grown...
Legumes can obtain nitrogen from symbiotic fixation in root nodules. The glutamine synthetase/glutamate synthase cycle is responsible for the initial assimilation. This work reports analysis of transgenic Lotus japonicus plants with chimeric gene containing alfalfa cytosolic synthetase (GS 1 ) (EC 6.3.1.2) controlled by Sesbania rostrata leghemoglobin promoter ( Sr g lb 3p). Surprisingly, all primary transformants analysed were sterile. Two designated GS39 and GS44 further analysed. GS...
Abstract A survey of entomopathogenic nematodes was conducted in sugar cane crops a total 14 soils, and positive results were obtained for strain MC5-2014 the municipality Tepalcingo, Morelos, soil with sandy loam texture pH 6.4. Species determination performed via morphological morphometric techniques by searching tubular stoma swollen cylindrical pharyngeal body metacorpus basal part. The range length ( L ) 750 to 1200 μm females 720 910 males, while corresponding maximum widths W 30 60 20...
White-rot fungi are efficient lignin degraders due to the secretion of peroxidase, manganese laccase, and versatile peroxidase (VP) on decayed wood. The VP is a high-redox-potential enzyme could be used detoxify reactive oxygen species (ROS), which accumulate in plants during biotic abiotic stresses. We cloned gene expressed it via Agrobacterium transformation procedure transgenic tobacco assay their tolerance different stress conditions. Thirty independent T2 lines overexpressing fungal...