A5029163577- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Protein Structure and Dynamics
- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- DNA Repair Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Advanced Nanomaterials in Catalysis
- Bacterial biofilms and quorum sensing
- Viral-associated cancers and disorders
- Genetics and Neurodevelopmental Disorders
- Cardiomyopathy and Myosin Studies
- Mechanical Engineering Research and Applications
- ATP Synthase and ATPases Research
- Enzyme-mediated dye degradation
- Various Chemistry Research Topics
- Computational Drug Discovery Methods
- Enzyme Production and Characterization
- Bone and Dental Protein Studies
- Ion Channels and Receptors
- Oral and Maxillofacial Pathology
- Cultural Heritage Materials Analysis
Institute of Molecular Biology of the Slovak Academy of Sciences
2010-2023
Slovak Academy of Sciences
2009-2023
National Institute of Amazonian Research
2020
Universidade Federal Rural de Pernambuco
2020
Universidade Federal de Campina Grande
2020
Universidad Nacional Autónoma de México
2020
Cardiff University
2010
Human ryanodine receptor 2 (hRyR2) mediates calcium release from the sarcoplasmic reticulum, enabling cardiomyocyte contraction. The N-terminal region of hRyR2 (amino acids 1-606) is target >30 arrhythmogenic mutations and contains a binding site for phosphoprotein phosphatase 1. Here, solution crystal structures determined under near-physiological conditions, as well homology model region, are presented. N-terminus held together by unique network interactions among its three domains, A, B...
Although the mechanism of RNA cleavage by RNases has been studied for many years, there remain aspects that have not yet fully clarified. We solved crystal structures RNase Sa2 in apo form and complexes with mononucleotides. These provide more details about Sa2. In addition to Glu56 His86, which are principal catalytic residues, an important role first reaction step also seems be played Arg67 Arg71, located phosphate-binding site hydrogen bonds oxygens phosphate group Their positive charge...
We report the domain analysis of N-terminal region (residues 1-759) human cardiac ryanodine receptor (RyR2) that encompasses one discrete RyR2 mutation clusters associated with catecholaminergic polymorphic ventricular tachycardia (CPVT1) and arrhythmogenic right dysplasia (ARVD2). Our strategy utilizes a bioinformatics approach complemented by protein expression, solubility limited proteolytic digestion. Based on analysis, we designed series specific fragments for cloning overexpression in...
The human cardiac ryanodine receptor (hRyR2), the ion channel responsible for release of Ca2+ ions from sarcoplasmic reticulum into cytosol, plays an important role in muscle contraction. Mutations to this are associated with inherited arrhythmias. These mutations appear cluster distinct parts N-terminal, central and C-terminal areas channel. Here, we used molecular dynamics simulation examine effects three disease-associated N-terminal region, R414L, I419F R420W, have on a model residues...
The M3 protein encoded by murine gammaherpesvirus 68 (MHV-68) is a unique viral immunomodulator with high-affinity for broad spectrum of chemokines, key mediators responsible the migration immune cells to sites inflammation. currently being studied as very attractive and desirable tool blocking chemokine signalling involved in some inflammatory diseases cancers. In this study, we elucidated role residues E70 T272 binding chemokines examining effects E70A T272G mutations on ability...
Catalase-peroxidases (KatGs) are unique bifunctional oxidoreductases that contain heme in their active centers allowing both the peroxidatic and catalatic reaction modes. These originally bacterial enzymes broadly distributed among various fungi them to cope with reactive oxygen species present environment or inside cells. We used biophysical, biochemical, bioinformatics methods investigate differences between catalase-peroxidases originating thermophilic mesophilic from different habitats....
Human ryanodine receptor 2 (hRyR2) is a calcium ion channel present in the membrane of sarcoplasmic reticulum cardiac myocytes that mediates release ions from stores during excitation- contraction coupling. Disease-causing mutations hRyR2 are clustered into N-terminal (amino acids 1-600), central 2100-2500) and C-terminal 3900-5000) regions. These regions believed to be involved regulation gating. The region has been implicated regulating basal activity by interaction with region. This paper...
M3 proteins of 44 kDa murine gammaherpesviruses 68 and 72 (MHV-68, MHV-72) were identified as herpesvirus vCKBP-3, soluble inhibitors the host chemokine network providing a selective advantage for virus by inhibiting antiviral inflammatory response during both acute latent infection. The MHV-72 protein was found to contain single mutation (Asp307Gly) near its chemokine-binding domain differ from that MHV-68 in ability bind some human chemokines. In this study, we optimized expression...