A5077883603- Food composition and properties
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Microbial Metabolites in Food Biotechnology
- Proteins in Food Systems
- Enzyme Production and Characterization
- Plant Pathogenic Bacteria Studies
- Malaria Research and Control
- Phytase and its Applications
- Invertebrate Immune Response Mechanisms
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Radiomics and Machine Learning in Medical Imaging
- Legume Nitrogen Fixing Symbiosis
- Phytochemicals and Antioxidant Activities
- Genomics and Chromatin Dynamics
- Polysaccharides Composition and Applications
- Potato Plant Research
- Trypanosoma species research and implications
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Biochemical and Structural Characterization
- Plant Micronutrient Interactions and Effects
- Genomics, phytochemicals, and oxidative stress
Canada's Michael Smith Genome Sciences Centre
2024-2025
University of British Columbia
2018-2024
BC Cancer Agency
2024
Agriculture and Agri-Food Canada
2014-2017
University of Guelph
2014-2017
Chlorogenic acid (CGA) exists as multiple isomers (e.g., 3-CQA, 4-CQA, 5-CQA, 3,4-diCQA, 3,5-diCQA, and 4,5-diCQA) in foods such coffee beverages, fruits vegetables. This study aimed to investigate relative activities of these six different CGA modify redox biology inflamed Caco-2 cells that involved Nrf2 signaling. were pre-treated with individual assess the effectiveness mitigate oxidative stress. Isomer-specific capacity for direct free radical scavenging activity potential endogenous...
The Long-Read Personalized OncoGenomics (POG) dataset comprises a cohort of 189 patient tumors and 41 matched normal samples sequenced using the Oxford Nanopore Technologies PromethION platform. This from POG program Marathon Hope Cancer Centres Network includes DNA RNA short-read sequence data, analytics, clinical information. We show potential long-read sequencing for resolving complex cancer-related structural variants, viral integrations, extrachromosomal circular DNA. Long-range phasing...
Cranberry beans from regular (RR) and nondarkening (CND) genotypes were pressure cooked, free, conjugated, bound phenolics analyzed. Simulated in vitro gastrointestinal digestion was used to assess the bioaccessibility of these phenolic fractions. Total content decreased after cooking digestion, whereas individual compounds affected differently. Cooking significantly increased release ferulic sinapic acids flavanols, released p-coumaric, ferulic, both genotypes, p-hydroxybenzoic acid,...
Abstract The emerging use of immunotherapy for cancer treatment has led to a greater need characterizing markers response. Incorporating immune profiling tools into whole genome and transcriptome sequencing analysis (WGTA) pipelines facilitates the identification biomarkers response that may be used inform clinical trial design. Personalized OncoGenomics (POG) program enrolled >1,200 patients with advanced/metastatic cancers diverse histologies underwent WGTA, which allowed...
Plasmodium falciparum plasmepsin X (PfPMX), involved in the invasion and egress of this deadliest malarial parasite, is essential for its survival hence considered as an important drug target. We report first crystal structure PfPMX zymogen containing a novel fold prosegment. A unique twisted loop from prosegment arginine 244 mature enzyme inactivation; such mechanism, not previously reported, might be common apicomplexan proteases similar to PfPMX. The maturation occurs through cleavage at...
Computational docking reveals the influence of conformation on inhibition cytochrome P450 3A4. Such structure–activity relationships are dependent intra-molecular interactions in cannabinoids, forming stabilised coiled structures.
The Long-read POG dataset comprises a cohort of 189 patient tumours and 41 matched normal samples sequenced using the Oxford Nanopore Technologies PromethION platform. This from Personalized Oncogenomics (POG) program Marathon Hope Cancer Centres Network includes accompanying DNA RNA short-read sequence data, analytics, clinical information. We show potential long-read sequencing for resolving complex cancer-related structural variants, viral integrations, extrachromosomal circular DNA....
Abstract The Long-read POG dataset comprises a cohort of 189 patient tumours and 41 matched normal samples sequenced using the Oxford Nanopore Technologies PromethION platform. This from Personalized Oncogenomics (POG) program Marathon Hope Cancer Centres Network includes accompanying DNA RNA short-read sequence data, analytics, clinical information. We show potential long-read sequencing for resolving complex cancer-related structural variants, viral integrations, extrachromosomal circular...
Epigenetic aberrations are linked to sporadic breast cancer. Interestingly, certain dietary polyphenols with anti-cancer effects, such as pterostilbene (PTS), have been shown regulate gene expression by altering epigenetic patterns. Our group has proposed the involvement of DNA methylation and methyltransferase 3B (DNMT3B) vital players in PTS-mediated suppression candidate oncogenes suggested a role enhancers target regions. In present study, we assess genome-wide impact PTS on marks at...
Summary Shepody potatoes were cooked using three common cooking methods – microwaving, boiling and pressure cooking. Microwaving for 2.5 min retained the highest amounts of slowly digestible starch ( SDS , 19.6%) resistant RS 48.8%) as compared to other treatments. Similarly, enthalpy FTIR results (ratio 1047/1022 cm −1 ) also higher microwaved samples, again indicating incomplete gelatinisation. Potatoes boiled 15, 30 or 45 min, followed by 1, 3 7 days retrogradation at 23 4 °C....
In plants, many natural defense mechanisms include cellular membrane fusion as a way to resist infection by external pathogens. Several plant proteins mediate fusion, but the detailed mechanism which they promote is less clear. Understanding this process could provide valuable insights into these proteins' physiological functions and guide bioengineering applications (i.e. design of antimicrobial proteins). The plant-specific insert (PSI) from Solanum tuberosum can help reduce certain...
The Solanum tuberosum plant specific insert (StPSI) has a defensive role in potato plants, with the requirements of acidic pH and anionic lipids. StPSI contains set three highly conserved disulfide bonds that bridge protein’s helical domains. Removal these leads to enhanced membrane interactions. This work examined effects their sequential removal, both individually combination, using all-atom molecular dynamics elucidate linkages maintaining overall protein tertiary structure. structure was...