A5042571263- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Nanoparticles: synthesis and applications
- Protein Structure and Dynamics
- Chemical Synthesis and Analysis
- Nanoparticle-Based Drug Delivery
- Ion-surface interactions and analysis
- Machine Learning in Bioinformatics
- RNA and protein synthesis mechanisms
- Venomous Animal Envenomation and Studies
- Nicotinic Acetylcholine Receptors Study
- Analytical chemistry methods development
- Essential Oils and Antimicrobial Activity
- Graphene and Nanomaterials Applications
- Spectroscopy and Quantum Chemical Studies
- Genomics and Phylogenetic Studies
- X-ray Diffraction in Crystallography
- interferon and immune responses
- Cardiac Fibrosis and Remodeling
- Crystallization and Solubility Studies
- Identification and Quantification in Food
- COVID-19 Impact on Reproduction
- Ion channel regulation and function
University of Southern Denmark
2016-2025
Odense University Hospital
2010-2024
Universidade Federal de Minas Gerais
2013-2020
Odense Municipality
2015-2019
Norwegian Institute of Bioeconomy Research
2011-2018
Charité - Universitätsmedizin Berlin
2013
University of Florida
2013
Universidade Federal de Ouro Preto
2013
Uppsala University
2003-2008
Aarhus University
2003
The renin-angiotensin system (RAS) is a key regulator of the cardiovascular system, electrolyte, and water balance. Here, we report identification characterization alamandine, new heptapeptide generated by catalytic action angiotensin-converting enzyme-2 angiotensin A or directly from angiotensin-(1-7).To characterize novel component RAS, alamandine.Using mass spectrometry observed that alamandine circulates in human blood can be formed angiotensin-(1-7) heart. Alamandine produces several...
The use of nanoparticles in foods, materials, and clinical treatments has increased dramatically the past decade. Because possibility human exposure to nanoparticles, there is an urgent need investigate molecular mechanisms underlying cellular responses that might be triggered. Such information necessary assess potential health risks arising from for developing new formulations next generation treatments. Using mass spectrometry-based proteomic technologies complementary techniques (e.g.,...
Electron-capture dissociation (ECD) is a new fragmentation technique that utilizes ion–electron recombination reactions. The latter have parallels in other research fields; revealing these helps to understand the ECD mechanism. An overview given of ECD-related phenomena and history discovery development. Current views on mechanism are discussed using both published examples.
The conventional approach in modern proteomics to identify proteins from limited information provided by molecular and fragment masses of their enzymatic degradation products carries an inherent risk both false positive negative identifications. For reliable identification even known proteins, complete de novo sequencing peptides is desired. main problems based on tandem mass spectrometry are incomplete backbone fragmentation the frequent overlap masses. In this work, first proteomics-grade...
An electron injection system based on an indirectly heated ring-shaped dispenser cathode has been developed and installed in a 7 Tesla Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. This new hardware design allows high-rate capture dissociation (ECD) to be carried out by hollow beam coaxial with the (ICR) trap. Infrared multiphoton (IRMPD) can also performed on-axis IR-laser passing through hole at centre of cathode. Electron photon irradiation times order 100 ms are...
Hydrogen rearrangement is an important process in radical chemistry. A high degree of H· to and from z· ionic fragments (combined occurrence frequency 47% compared with that z·) confirmed analysis 15,000 tandem mass spectra tryptic peptides obtained electron capture dissociation (ECD), including previously unreported double losses. Consistent the character abstraction, residue determining formation rate z′=z·+H· species found be N-terminal species. The size complementary c′m fragment turned...
Carrots (Daucus carota L.) of cv. Bolero and Carlo were processed into shreds stored for up to 4 months at −24 °C (frozen storage), or the roots 1 (refrigerated storage) followed by processing shreds. Volatiles from carrot collected dynamic headspace technique analyzed GC-FID, GC-MS, GC-MS/MS, GC-O determine volatile composition aroma active components carrots under different temperature conditions. A total 52 compounds quantified, which mono- sesquiterpenes accounted ∼99% mass. Major...
The study of cellular dynamics by proteomics using mass spectrometry requires a quantitation strategy that is robust, sensitive, and sufficient resolution to deal with subtle changes in protein expression or post-translational modification. major strategies are stable isotopic labeling proteins peptides for vitro cell culture systems (stable isotope amino acids culture, SILAC) isobaric peptide labels such as tags relative absolute (iTRAQ) tandem (TMT) both vivo systems. These have also been...
Fragmentation of peptide polyanions by electron detachment dissociation (EDD) has been induced irradiation deprotonated polypeptides [M-nH](n-) with >10 eV electrons. EDD found to lead preferentially a* and x fragment ions (C(alpha)-C backbone cleavage) arising from the oxidized radical anions [M-nH]((n-1)-*. We demonstrate that C(alpha)-C cleavages, which are otherwise rarely observed in tandem mass spectrometry, can account for most fragmentation, even-electron fragments dominating over...
We have tested the effect of m-nitrobenzyl alcohol (m-NBA) as a method to increase average charge state protonated gas-phase molecular ions generated by ESI from tryptic peptides and phosphopeptides. Various concentrations m-NBA were added mobile phases liquid chromatography system coupled an tandem mass spectrometer. Addition just 0.1% changed for identified BSA 2.2+ 2.6+. As result, predominant states 2+ ≥3+. To evaluate benefits peptide enhancement, ETD fragmentation efficiency Mascot...
We present basic workups and quantitative comparisons for two current generation Orbitrap mass spectrometers, the Q Exactive Plus Fusion Tribrid, which are widely considered of highest performing instruments on market. assessed performance methods both instruments, namely label-free quantitation stable isotope labeling using isobaric tags, studying heat shock response in Escherichia coli. investigated recently reported MS3 method instrument potential MS3-based reporter ion isolation...
Silver nanoparticles (AgNPs) have been reported to penetrate the central nervous system (CNS) and induce neurotoxicity. However, there is a paucity of understanding toxicity AgNPs their effect on blood-brain barrier (BBB) including underlying molecular mechanism(s) action. Such information important for formulation new strategies delivery biological therapeutics targets. Using an in vitro BBB model mass spectrometry-based proteomics, we investigated alterations proteomes brain endothelial...
Nanoparticles (NPs) in contact with protein-containing media such as biological fluids rapidly acquire a surface layer of proteins, known the protein corona. The composition and structural properties corona are crucial for NP interactions living cells. Although much has been learned about phenomenon, further elucidation could benefit from extensive quantitative proteomics analysis. Herein we report comprehensive characterization (>350 proteins) that formed on 60 nm silver NPs via interaction...
Aim: Development of fluorometholone-loaded PEG-PLGA nanoparticles (NPs) functionalized with cell-penetrating peptides (CPPs) for the treatment ocular inflammatory disorders. Materials & methods: Synthesized polymers and were used elaboration NPs, which characterized physicochemically. Cytotoxicity ability to modulate expression proinflammatory cytokines evaluated in vitro using human corneal epithelial cells (HCE-2). NPs uptake was assayed both vivo models. Results: showed physicochemical...
Proteome characterization relies heavily on tandem mass spectrometry (MS/MS) and is thus associated with instrumentation complexity, lengthy analysis time, limited duty cycle. It was always tempting to implement approaches that do not require MS/MS, yet they were constantly failing achieve a meaningful depth of quantitative proteome coverage within short experimental times, which particularly important for clinical or biomarker-discovery applications. Here, we report the first successful...
In hot electron capture dissociation (HECD), multiply protonated polypeptides fragment upon capturing ∼11-eV electrons. The excess of energy the primary c, z• cleavage induces secondary fragmentation in fragments. resultant w ions allow one to distinguish between isomeric Ile and Leu residues. analytical utility HECD is evaluated using tryptic peptides from bovine milk protein PP3 containing totally 135 amino acid Using a formal procedure for Ile/Leu (Xle) residue assignment, identities 20...
A comprehensive approach to protein identification and determination of sites posttranslational modifications (PTMs) in heavily modified proteins was tested. In this approach, termed "reconstructed molecular mass analysis" (REMMA), the distribution intact is measured first, which reveals extent heterogeneity modifications. Then digested with one or several enzymes, peptides separated by reversed-phase HPLC, analyzed Fourier transform spectrometry (FTMS). Vibrational excitation (collisional...
Abstract New low‐energy electron injection systems based on indirectly heated dispenser cathodes facilitate capture dissociation (ECD) in Fourier transform ion cyclotron resonance (FTICR) mass spectrometry. In this joint report, details are presented of the design and performance these two commercial FTICR instruments, 9.4 T Bruker BioAPEX Uppsala 4.7 IonSpec Ultima Odense. results include obtaining meaningful one‐scan MS/MS data for isolated precursor ions with millisecond irradiation...
Overall charge unchanged: Disulfide bonds in gaseous polypeptide cations can be cleaved efficiently by ultraviolet photodissociation (UVPD) at 157 nm (see scheme). Unlike electron-capture dissociation, UVPD cleaves SS without overall reduction the product ions. This cleavage is suggested to a result of electronic excitation caused absorption photons 7.9 eV. Supporting information for this article available on WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z501533_s.pdf or from...
First results are reported on the application of ECD in analysis 2+ and 3+ ions stereoisomers Trp-cage (NLYIQWLKDGGPSSGRPPPS), smallest fastest-folding protein, which exhibits a tightly folded tertiary structure solution. The chiral recognition based ratios abundances z18 z19 fragments was excellent even for single amino acid (Tyr) D-substitution (Rchiral=8.6). effect decreased with an increase temperature at electrospray ion source, as well higher degree ionization, (Rchiral=1.5). A general...
Proteome-wide analyses rely on tandem mass spectrometry and the extensive separation of proteolytic mixtures. This imposes considerable instrumental time consumption, which is one main obstacles in broader acceptance proteomics biomedical clinical research. Recently, we presented a fast proteomic method termed DirectMS1 based ultrashort LC gradients as well MS1-only spectra acquisition data processing. The allows significant reduction proteome-wide analysis to few minutes at depth...
Recently, we presented the DirectMS1 method of ultrafast proteome-wide analysis based on minute-long LC gradients and MS1-only mass spectra acquisition. Currently, provides depth human cell proteome coverage 2500 proteins at a 1% false discovery rate (FDR) when using 5 min 7.3 runtime in total. While standard MS/MS approaches provide 4000–5000 protein identifications within couple hours instrumentation time, advocate here that higher number identified does not always translate into better...
Complementary cleavage: A large-scale (ca. 15 000 spectra) comparison between the cleavage sites and preferences of collision-activated dissociation (CAD) electron-capture (ECD) in tandem mass spectrometry has proved beyond doubt their complementary nature (see picture, red: high, blue: low). The study also suggested presence a preferred N-terminal structure gas-phase tryptic peptide dications.