The anti-tumor activity of diosgenin, a new steroidal constituent present in fenugreek, on two human breast cancer cell lines, MCF-7 and Hs578T, was studied. Diosgenin treatment resulted growth inhibition, cycle arrest, apoptosis concentration- time-dependent manners both lines. Western blot analyses whole lysates for proteins showed that diosgenin altered phosphorylated cyclin checkpoint1 (p-Chk1Ser345) B expression, which G2/M phase blockade. Mechanistically, Cdc25C-Cdc2 signaling involved...

Oxidative stress and inflammation are related to several chronic diseases including cancer atherosclerosis. Hibiscus sabdariffa Linnaeus has been found possess antioxidant effects. In this study, polyphenols extracted from L. (HPE) were used detect anti-inflammatory effects on nitrite prostaglandin E2 (PGE2) in lipopolysaccharide (LPS) treated RAW264.7 cells. Sequentially, an animal model examination was performed confirm the of HPE LPS-induced hepatic inflammation. The results showed that...

Elevated serum levels of uric acid are associated with an increased risk for hyperuricemia, gout, hypertension, cardiovascular disease, and renal failure. Recent attention has focused on the bioactive properties edible plants in preventing disease. Hibiscus sabdariffa L., a local soft drink material medicinal herb Taiwan, is used effectively native medicines against pyrexia, liver disorders. We investigated effects extract (HSE) oxonic (OA)-induced hyperuricemia rats. The HSE affected urate...

Background /Aims: Recent studies of microRNA (miRNA) involvement in tumorigenesis have indicated the critical role these non-coding small RNAs malignant transformation, but prognostic role, if any, miRNAs breast cancer remains undetermined. Therefore, we assessed significance microRNA-9 (miR-9) and miR-221 toward goal understanding contribution(s) to cell stemness. Methods: The level each miR-9 206 paired laser capture microdissected tumor cells non-tumor was determined by quantitative...

Mulberry leaves (Morus alba L.), which are traditional Chinese herbs, exert several biological functions, such as antioxidant, anti-inflammation, antidiabetic, and antitumor.Alcohol intake increases inflammation oxidative stress, this increase causes liver injury leads to steatosis, cirrhosis, hepatocellular carcinoma, major health problems worldwide.Previous report indicated that mulberry leaf extract (MLE) exited hepatoprotection effects against chronic alcohol-induced damages.In present...

Purple sweet potato (PSP) powder with anthocyanins possesses the ability to reduce oxidative stress and inflammation. Studies have presumed a positive correlation between body fat dry eye disease (DED) in adults. The regulation of inflammation has been proposed as mechanism underlying DED. This study developed an animal model high diet (HFD)-induced We added 5% PSP HFD evaluate effects mechanisms mitigating HFD-induced A statin drug, atorvastatin, was also separately assess its effect....

Oxidative stress is the major contributor to acetaminophen (AAP)-caused liver damage. It promotes mitochondrial oxidative and collapses membrane potential cause cell death. We have previously shown that a polyphenol extract of Hibiscus sabdariffa L. (HPE) potentiated antioxidative effect. further examined in this study possible mechanism HPE against AAP-caused BABL/c mice were orally fed with (100, 200 or 300 mg/kg) for two weeks prior an i.p. injection 1000 mg/kg AAP. The decapitated 6 h...

Cell cycle regulation is a critical issue in cancer treatment. Previously, gallic acid (GA) has been reported to possess anticancer ability. Here, we have evaluated the molecular mechanism of GA on cell modulation human bladder transitional carcinoma line (TSGH-8301 cell).Using flow cytometer analysis, exposure cells 40 μM resulted statistically significant increase G2/M phase cells, which was accompanied by decrease G0/G1 cells. GA-treated growth inhibition dose-dependent manner...

The NLPE and GA potentially prevent atherosclerosis by inhibiting VSMC migration proliferation. mechanisms involve the regulation of miRNA in PTEN, Ras/extracellularsignal-regulated kinase pathway, Rho family proteins.

The stemness and metastasis of cancer cells are crucial features in determining progression. Argonaute-2 (AGO2) overexpression was reported to be associated with microRNA (miRNA) biogenesis, supporting the self-renewal differentiation characteristics stem (CSCs). Ursolic acid (UA), a triterpene compound, has multiple biological functions, including anticancer activity. In this study, we find that UA inhibits proliferation MDA-MB-231 MCF-7 breast cell lines using CCK-8 assay. induced...

Diabetic nephropathy (DN) is a major cause of morbidity and mortality among patients with diabetes mellitus (DM). Studies have highlighted the critical role reactive oxygen species (ROS) in pathogenesis DM its complications. Apple polyphenol (AP) has demonstrated antioxidant properties various models. In this study, we investigated effects AP on DN rat model. Type 1 was induced Sprague–Dawley rats via single intraperitoneal injection streptozotocin (65 mg/kg) (n = 8). Rats blood glucose...

Accumulation of visceral fat has been reported to increase systemic inflammation. Purple sweet potato (Ipomoea batatas L., PSP), known for its anthocyanin content, potentiates in mitigating oxidative stress. This study aimed investigate the underlying mechanisms by which PSP influences body deposition. Five-week-old male Sprague-Dawley rats (n = 5) were fed a 43% high-fat diet (HFD) 2 weeks induce obesity, followed 19 HFD supplemented with 5% PSP. significantly improved weight and reduced...

Several studies point out that oxidative stress maybe a major culprit in diabetic nephropathy. Aqueous extract of Hibiscus sabdariffa L. (HSE) has been demonstrated as having beneficial effects on anti-oxidation and lipid-lowering experimental studies. This study aimed at investigating the nephropathy streptozotocin induced type 1 rats. Our results show HSE is capable reducing lipid peroxidation, increasing catalase glutathione activities significantly kidney, decreasing plasma levels...

Abstract BACKGROUND: Acetaminophen (AAP)‐induced oxidative stress can cause cell death to induce liver damage. The antioxidant effect of Hibiscus sabdariffa L. (HS) was shown in previous studies. In this study the HS extract (HSE) on AAP‐induced injury BALB/c mice investigated. RESULTS: vivo , were fed orally with 200, 400 or 600 mg kg −1 HSE for 2 weeks and then injected 1000 AAP. Pretreatment decreased lipid peroxidation increased catalase activity glutathione level. It also injury,...

Dysregulated T helper (Th) cells are considered important in the pathophysiology of chronic primary immune thrombocytopenia (ITP). The present study investigated whether levels Th cytokines pediatric patients with ITP were different compared healthy controls.Fifty-seven and 28 controls enrolled. Patients divided into three groups based on their platelet counts at time study: (i) active disease <50 × 10(9) /l (n = 23), (ii) stable 50-150 (iii) remission >150 11). Plasma concentration Th1...

Shi-Liu-Wei-Liu-Qi-Yin (SLWLQY) was traditionally used to treat cancers. However, scientific evidence of the anticancer effects still remains undefined. In this study, we aimed clarify possible mechanisms SLWLQY in treating cancer. We evaluated on apoptosis-related experiments inducing TSGH-8301 cells by (i) 3-(4,5-dimethylthiazol-zyl)-2,5-diphenylterazolium bromide (MTT) for cytotoxicity; (ii) cell-cycle analysis and (iii) western blot G2/M-phase apoptosis regulatory proteins. Human bladder...

The polyphenols in mulberry leaf possess the ability to inhibit cell proliferation, invasion, and metastasis of tumors. It was reported that p53 status plays an important role switching apoptosis cycle following adenosine monophosphate-activated protein kinase (AMPK) activation. In this study, we aimed detect effect polyphenol extract (MLPE) on inducing death p53-negative (Hep3B) p53-positive (Hep3B with transfected p53) hepatocellular carcinoma cells also clarify MLPE-treated cells. After...

Mulberry water extracts (MWEs), which contain polyphenols including anthocyanins, have been used in traditional Chinese edible food. The hepatoprotective effects and molecular mechanisms of MWEs on acute liver failure induced by lipopolysaccharides (LPS) were investigated vivo.Rats administered different doses (0.5 1 g kg(-1)) h before injection LPS (5 mg then sacrificed 10 after treatment with LPS. Liver function, inflammatory factors, oxidative stress index hepatic histopathological...