A5049219495- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Plant Gene Expression Analysis
- Hemoglobin structure and function
- Horticultural and Viticultural Research
- Coastal wetland ecosystem dynamics
- Plant Ecology and Soil Science
- Invertebrate Taxonomy and Ecology
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Plant responses to elevated CO2
- Genomics and Phylogenetic Studies
- Seed Germination and Physiology
- Potato Plant Research
- Polymer-Based Agricultural Enhancements
- Enzyme-mediated dye degradation
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Plant Micronutrient Interactions and Effects
- Aluminum toxicity and tolerance in plants and animals
- Mycorrhizal Fungi and Plant Interactions
- Lignin and Wood Chemistry
- Forest ecology and management
University of Helsinki
2009-2022
Biocenter Finland
2000-2016
Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make an attractive model for forest biotechnology. We assembled chromosomally anchored the nuclear genome of inbred B. pendula individual. Gene duplicates from paleohexaploid event were enriched transcriptional regulation, whereas tandem overrepresented by environmental responses. Population resequencing 80...
Hydrogen peroxide (H2O2) was detected cytochemically in plant tissues during anoxia and re-oxygenation by transmission electron microscopy using its reaction with cerium chloride to produce dense precipitates of perhydroxides. Anoxia-tolerant yellow flag iris (Iris pseudacorus) rice (Oryza sativa), anoxia-intolerant wheat (Triticum aestivum) garden germanica) were used the experiments. In all plants tested, increased H2O2 plasma membranes apoplast. anoxia-tolerant species response delayed...
Summary Tree bark is a highly specialized array of tissues that plays important roles in plant protection and development. Bark develop from two lateral meristems; the phellogen (cork cambium) produces outermost stem–environment barrier called periderm, while vascular cambium contributes with phloem tissues. Although diverse terms tissues, functions species, it remains understudied at higher resolution. We dissected stem silver birch ( Betula pendula ) into eight major tissue types,...
Summary Organellar reactive oxygen species ( ROS ) signalling is a key mechanism that promotes the onset of defensive measures in stress‐exposed plants. The underlying molecular mechanisms and feedback regulation loops, however, still remain poorly understood. Our previous work has shown specific regulatory B′γ subunit protein phosphatase 2A PP 2A) required to control organellar associated metabolic adjustments Arabidopsis thaliana . Here, we addressed through which 2A‐B′γ impacts on...
A comparative transcriptomic study and a single-cell metabolome analysis were combined to determine whether parenchymal ray cells contribute the biosynthesis of monolignols in lignifying xylem Norway spruce (Picea abies). Ray may function lignification upright tracheids by supplying monolignols. To test this hypothesis, dissected with laser-capture microdissection from tangential cryosections developing trees. The transcriptome revealed that among genes involved processes typical for...
Peroxidation was studied in anoxically treated plant tissues and quantified as conjugated dienes/trienes the total lipid fraction production of thiobarbituric acid reactive substances (TBARS). Oxidative stress caused by re‐exposure plants to oxygen led an increase diene/triene formation rhizomes Iris germanica roots wheat ( Triticum aestivum L.) oats Avena sativa L.), after a long anoxic exposure (45 days) very anoxia tolerant pseudacorus . Second derivative (SD) spectrophotometry UV...
Laser capture microdissection (LCM) enables precise dissection and collection of individual cell types from complex tissues. When applied to plant cells, especially woody tissues, LCM requires extensive optimization overcome such factors as rigid walls, large central vacuoles, intercellular spaces, technical issues with thickness flatness the sections. Here we present an optimized protocol for laser-assisted developing xylem mature trees: a gymnosperm (Norway spruce, Picea abies) angiosperm...
Both the mechanisms of monolignol transport and transported form monolignols in developing xylem trees are unknown. We tested hypothesis an active, plasma membrane-localized monomers, dimers, and/or glucosidic forms with membrane vesicles prepared from lignin-forming tissue-cultured cells Norway spruce (Picea abies L. Karst.), as well control materials, comprising non-lignifying phloem tobacco (Nicotiana tabacum L.) BY-2 cells. Xylem both coniferin p-coumaryl alcohol glucoside, but inhibitor...
The redox potential of the cell, as well antioxidant status tissue, are considered to be important regulatory constituents in an adaptive response plants. Here involvement active antioxidants ascorbic acid (AA), reduced glutathione (GSH) and α ‐ β ‐tocopherols reactive oxygen species scavenging, effect anoxic stress on their reduction state were studied 4 anoxia‐tolerant ‐intolerant plant species: Iris germanica L., pseudacorus wheat ( Triticum aestivum L. cv. Leningradka) rice Oryza sativa...
The Arabidopsis mutant rcd1 is tolerant to methyl viologen (MV). MV enhances the Mehler reaction, i.e. electron transfer from Photosystem I (PSI) O 2 , generating reactive oxygen species (ROS) in chloroplast. To study tolerance of we first addressed chloroplast thiol redox enzymes potentially implicated ROS scavenging. NADPH-thioredoxin oxidoreductase type C (NTRC) was more reduced . NTRC contributed photosynthetic and metabolic phenotypes but did not determine its tolerance. We next tested...
Hydrogen peroxide (H2O2) was detected cytochemically in plant tissues during anoxia and re‐oxygenation by transmission electron microscopy using its reaction with cerium chloride to produce dense precipitates of perhydroxides. Anoxia‐tolerant yellow flag iris (Iris pseudacorus) rice (Oryza sativa), anoxia‐intolerant wheat (Triticum aestivum) garden germanica) were used the experiments. In all plants tested, increased H2O2 plasma membranes apoplast. anoxia‐tolerant species response delayed...
Six potato cultivars grown in Turkey boron-prone areas and differing their tolerance towards high boron were studied to reveal whether causes oxidative stress. To assess stress level, chlorophyll fluorescence growth parameters measured. Oxidative damage was assessed as malondialdehyde antioxidant protection evaluated ascorbate (AA), dehydroascorbate, reduced glutathione (GSH) oxidized amounts superoxide dismutase, catalase, peroxidase (APX) reductase (GR) activities. High affected...
In the version of this article initially published, there was a mistake in calculation nucleotide mutation rate per site generation: 1 × 10−9 mutations generation used, whereas 9.5 correct. This error affects interpretation population-size changes over time and their possible correspondence with known geological events, as shown original Fig. 4 supporting discussion text, well details Supplementary Note. Neither data themselves nor any other results are affected. Figure has been revised...
Interactions between plant energy organelles, the chloroplasts and mitochondria, are crucial for development acclimation. These interactions occur at different levels including exchange of metabolites reducing power, organelle signaling pathways intracellular gas exchange. Mitochondrial retrograde stress activates expression nuclear genes encoding mitochondrial components, alternative oxidases. High abundances these respiratory enzymes coincide not only with changes in respiration but also...
Abstract Mitochondrial retrograde signals control expression of nuclear mitochondrial dysfunction stimulon (MDS) genes. Although MDS gene products mostly affect functions, they also influence production reactive oxygen species (ROS) and redox status chloroplasts. To study this inter-organellar interaction, we analysed the response Arabidopsis MDS-overexpressor mutant rcd1 to methyl viologen (MV), which catalyses electron transfer from Photosystem I (PSI) molecular oxygen, generating ROS in...
Plant respiratory burst oxidase homologs (RBOHs) are plasma membrane-localized NADPH oxidases that generate superoxide anion radicals, which then dismutate to H2O2, into the apoplast using cytoplasmic as an electron donor. PaRBOH1 is most highly expressed RBOH gene in developing xylem well a lignin-forming cell culture of Norway spruce (Picea abies L. Karst.). Since no previous information about regulation gymnosperm RBOHs exist, our aim was resolve how regulated with focus on...