A5070703862- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Myxozoan Parasites in Aquatic Species
- Aquaculture Nutrition and Growth
- Marine Bivalve and Aquaculture Studies
- Microbial infections and disease research
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Aquatic Invertebrate Ecology and Behavior
- Neurobiology and Insect Physiology Research
- Biosensors and Analytical Detection
- Parasite Biology and Host Interactions
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- Parasitic Infections and Diagnostics
- Physiological and biochemical adaptations
- Pharmaceutical and Antibiotic Environmental Impacts
- Aquatic life and conservation
- Vibrio bacteria research studies
- Viral Infections and Vectors
- Fish Ecology and Management Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Okayama University of Science
2022-2025
Japan Fisheries Research and Education Agency
2015-2024
University of Miyazaki
2006-2011
Red sea bream iridoviral disease (RSIVD) causes high economic damage in mariculture Asian countries. However, there is little information on the source of infection and viral dynamics fish farms. In present study, RSIV a farm that mainly reared juveniles broodstocks red (Pagrus major) were monitored over 3 years (2016 to 2018) by targeting environmental DNA (eDNA) seawater. Our monitoring demonstrated iridovirus (RSIV) was detected from eDNA at least 5 days before an RSIVD outbreak...
White spot syndrome virus (WSSV) is a highly infectious that poses an imminent threat to global shrimp aquaculture and responsible for significant mortality in farms. There lack of targeted drug therapy options preventing or treating WSSV. Consequently, these envelope proteins have garnered attention as alternative focus development at the molecular level. In current study, binding efficiency pregnane‐3,20‐dione, 17,21‐[(methylborylene)bis(oxy)]‐, (5. beta)‐, compound identified from...
White spot syndrome virus (WSSV) continues to be the most pathogenic among crustacean aquaculture causing mass mortality. In present study, we established a one-step, single tube, real-time accelerated loop-mediated isothermal amplification (real-time LAMP) for quantitative detection of WSSV.A set six specially designed primers that recognize eight distinct sequences target. The whole process can completed in 1 h under conditions at 63 degrees C. Detection and quantification achieved by...
Carp edema virus disease (CEVD), or koi sleepy disease, is caused by CEV. Here, we report the complete genome sequence of CEV strain FTI2020, isolated from carp. This information has great potential for improving our understanding genetic characteristics this piscine poxvirus.
In the present study, we have cloned a new family of anti-lipopolysaccharide factor (ALF) from haemocytes kuruma shrimp Marsupenaeus japonicus (MjALF2) using RACE method.Transcriptional analysis MjALF2 gene in organs healthy revealed prominent expression gills and muscle. vitro LPS stimulation lymphoid organ cells resulted significant increase at 48, 8 12 h poststimulation, compared to nonstimulated cells. vivo injection V. penaeicida does not show any high time course assay. Phylogenetic...
The Akoya pearl oyster (
In this study, we have developed a DNA vaccine encoding viral envelope protein VP28 of penaeid rod-shaped virus (PRDV, = WSSV). Protective efficacy the against PRDV was confirmed at 7 days post vaccination in kuruma shrimp Penaeus japonicus. The transcript derived from detected various tissues 1, 3 and vaccination. Moreover, notable up-regulated expression Rab7, penaeidin, lysozyme crustin genes observed upon These results suggest that significantly increased protection innate immune responses PRDV.
Abstract A quantitative rapid detection method based on loop‐mediated isothermal amplification has been developed for red‐spotted grouper nervous necrosis virus ( RGNNV ). The nested polymerase chain reaction PCR ) assay is the mainstream inspection of brooder in hatchery. In this study, a real‐time LAMP applied detection, known as high‐speed gene procedure. Of three temperatures (60 °C, 63 °C and 65 °C) attempted, it found that giving higher from 11th minute onwards. Sensitivity analysis...
Food poisoning of humans, caused by the ingestion raw flesh olive flounder Paralichthys olivaceus infected with Kudoa septempunctata, has recently become a public health concern in Japan. The present study investigated infection dynamics K. septempunctata two cohorts produced hatchery, where was enzootic, PCR assay and light microscopy. In less than 1-year-old juveniles 2011 cohort (hatched February 2011), not detected either June or July even conventional PCR, but microscopy heavy (> 1 ×...
Recent applications of RNA interference technology against the white spot syndrome virus (WSSV) were found to be promising and effective antiviral strategies. In present study, a gene-specific full-length double-stranded (dsRNA) was prepared for WSSV-VP28 gene using in vitro transcription method. Challenging experiments performed by an intramuscular injection VP28-dsRNA Marsupenaeus japonicus, followed WSSV infection after 24 h, expecting 95–100% mortality within 9 days duration. The results...