The spread of microplastics is a serious environmental problem, which attracts increasing attention. achievements analytical chemistry made it possible to accumulate and systematize an extensive array data on the contents in objects living (micro)organisms. current situation brings about challenge to, at least, isolate from and, most, efficiently decompose them. This review systematizes existing (bio)catalytic methods approaches that can be used for decomposition various microplastics....

Catalytic characteristics of hexahistidine-containing organophosphorus hydrolase (His6-OPH) and its enzyme-polyelectrolyte complexes with poly-l-glutamic acid or poly-l-aspartic (His6-OPH/PLD50), hydrolyzing organophosphorous compounds, N-acyl homoserine lactones were studied in the presence various antibiotics (ampicillin, gentamicin, kanamycin, rifampicin). The at concentrations below 1 g·L−1 had a negligible inhibiting effect on His6-OPH activity. Mixed inhibition was established for...

A new biocatalyst in the form of Komagataeibacter xylinum B-12429 cells immobilized poly(vinyl alcohol) cryogel for production bacterial cellulose was demonstrated. Normally, increased bacteria concentration causes an enlarged synthesis while push polysaccharide out to pack themselves into this polymer and go a stasis. Immobilization allowed “deceiving” them: producing pushed it out, which further passed through pores matrix accumulated medium not covering cells; hence, latter were deprived...

To obtain fiber materials with pronounced chemical-biological protection, metal (Zn or Ta) nanoparticles were jointly applied polyelectrolyte complexes of enzymes and polypeptides being their stabilizers. Computer modeling revealed the preferences between certain partners for N-acyl-homoserine lactone acylase hexahistidine-tagged organophosphorus hydrolase (His6-OPH) possessing quorum quenching (QQ) behavior bacterial cells. The combinations appeared to function better as compared same...

Combined use of various antimicrobial peptides (AMPs) with enzymes that hydrolyze the signaling molecules resistance mechanism microorganisms, quorum sensing (QS), to obtain effective antimicrobials is one leading approaches in solving problem. Our study investigates lactoferrin-derived AMPs, lactoferricin (Lfcin), lactoferampin and Lf(1-11), as potential partners for combination hydrolyzing lactone-containing QS molecules, hexahistidine-containing organophosphorus hydrolase (His6-OPH)...

The article deals with the issues of design and construction a laboratory stand ship boiler plant based on Kiturami TURBO17 boiler, located in “Ship Boiler Steam Generating Plants” Federal State Budgetary Educational Institution Higher Education “Astrakhan Technical University”. considered paper, made accordance requirements International Convention Standards Training, Certification Watchkeeping for Seafarers (STCW 78), was developed scientific research training students. presents...

The purpose of the study is selection stable, most decorative large-flowered seedlings panicled phlox and their description. As a result plants from seed progeny (Phlox paniculata) in 2022, original them were selected. greatest interest was represented by with large flowers – more than 4 cm diameter, various colors, beautiful inflorescences, general habit, resistant to adverse factors. paper characterizes describes, taking into account rules International Code Nomenclature Cultivated Plants,...

The possible use of the concentration intracellular adenosine triphosphate (ATP) as a parameter enabling quick and adequate evaluation metabolic activity methanogenic cells was demonstrated in work. This approach used to analyze effect introducing potassium humate fulvic acids (1–10 g/L) into media with four different consortia producing biogas. ATP analyzed by bioluminescent luciferin–luciferase method at beginning end process. During entire process, biogas composition, efficiency, kinetics...

Polysaccharides synthesized by microorganisms (bacterial cellulose, dextran, pullulan, xanthan, etc.) have a set of valuable properties, such as being antioxidants, detoxifying, structuring, biodegradable, etc., which makes them suitable for variety applications. Biocatalysts are the key substances used in producing polysaccharides; therefore, modern research is focused on composition and properties biocatalysts. determine possible range renewable raw materials can be substrates synthesis,...

This work was aimed at the development of an immobilized artificial consortium (IMAC) based on microorganisms belonging to Gram-positive and Gram-negative bacterial cells capable jointly carrying out rapid effective degradation different organophosphorus pesticides (OPPs): paraoxon, parathion, methyl diazinon, chlorpyrifos, malathion, dimethoate, demeton-S-methyl. A cryogel poly(vinyl alcohol) applied as a carrier for IMAC. After selection made between several candidates genera Rhodococcus...

Interest in enzymes capable of neutralizing various mycotoxins is quite high. The methods used for the screening and selection that catalyze detoxification should be sensitive fast. However toxic compounds can generated under action such enzymes. Thus, assessment overall reduction properties reaction media towards bioluminescent bacteria seems to most reasonable control method allowing a quick search effective enzymatic biocatalysts. influence wide range glucanases, which hydrolyze toxins...

Since the growing number of fungi resistant to fungicides used is becoming a serious threat human health, animals, and crops, there need find other effective approaches in eco-friendly suppression fungal growth. One main mechanisms development resistance fungi, as well bacteria, antimicrobial agents quorum sensing (QS), which various lactone-containing compounds participate signaling molecules. This work aimed study effectiveness action enzymes exhibiting lactonase activity against For this,...

We have performed studies and comparative analysis of the biosynthesis characteristics intracellular recombinant enzyme, such as hexahistidine-containing organophosphorus hydrolase (His6-OPH) in Escherichia coli SG13009[pREP4] cells when various perfluorocarbon compounds (PFC) were introduced into medium for cell cultivation. The PFC found to facilitate His6-OPH: increased levels total OPH-activity (up 37%) measured upon introduction 1,1,1,2,2,3,3,4,4,5,5,6,6,6-tetradecafluorohexane (PFH)...

To improve the action of already in use antibiotics or new antimicrobial agents against different bacteria, development effective combinations peptides (AMPs) with enzymes that can quench quorum (QQ) sensing bacterial cells was undertaken. Enzymes hydrolyzing N-acyl homoserine lactones (AHLs) and are signal molecules Gram-negative Gram-positive cells, respectively, were estimated as “partners” for newly designed antimicrobial–enzymatic combinations. The molecular docking six to surface 10 QQ...