A5081902361- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Bacterial Infections and Vaccines
- Viral gastroenteritis research and epidemiology
- Cellular transport and secretion
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Estrogen and related hormone effects
- Retinoids in leukemia and cellular processes
- Salmonella and Campylobacter epidemiology
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Neonatal and Maternal Infections
- Inflammatory mediators and NSAID effects
- Gene Regulatory Network Analysis
- Microbial infections and disease research
- Plant Virus Research Studies
- Microbial Community Ecology and Physiology
- Gut microbiota and health
- Cerebrospinal fluid and hydrocephalus
- Infective Endocarditis Diagnosis and Management
- Complement system in diseases
- Biochemical and Structural Characterization
- Evolution and Genetic Dynamics
- Evolutionary Game Theory and Cooperation
National University of Singapore
2025
Loyola University Chicago
2025
University of Lausanne
2023
Swiss Federal Institute of Aquatic Science and Technology
2021-2023
IRCCS Humanitas Research Hospital
2023
ETH Zurich
2021-2023
University of Arizona
2013-2018
Retraction of the type IV pilus (Tfp) mediates DNA uptake, motility, and social infection behavior in a wide variety prokaryotes. To date, investigations into Tfp retraction-dependent activities have used mutant deleted PilT, ATPase motor protein that causes fiber to retract. ΔpilT cells are nontransformable, nonmotile, cannot aggregate microcolonies. We tested hypothesis these sensitive strength PilT enzymatic activity by using pathogen Neisseria gonorrhoeae as model. constructed an N. with...
Salmonella spp. express pathogenicity island 1 Type III Secretion System (T3SS-1) genes to mediate the initial phase of interaction with their host. Prior studies indicate short-chain fatty acids, microbial metabolites at high concentrations in gastrointestinal tract, limit population-level T3SS-1 gene expression. However, only a subset cells population these genes, suggesting acids could decrease expression by acting on per-cell or proportion expressing cells. Here, we combine single-cell,...
Introduction The orphan nuclear receptor COUP-TFII plays an undefined role in breast cancer. Previously we reported lower expression tamoxifen/endocrine- resistant versus sensitive cancer cell lines. identification of COUP-TFII-interacting proteins will help to elucidate its mechanism action as a transcriptional regulator Results FLAG-affinity purification and multidimensional protein technology (MudPIT) identified nucleolin among the interacting with MCF-7 tamoxifen-sensitive cells....
Members of the genus Neisseria have been isolated from or detected in a wide range animals, non-human primates and felids to rodent, guinea pig. By means selective culture, biochemical testing, Gram staining PCR screening for Neisseria-specific internal transcribed spacer region rRNA operon, we four strains oral cavity wild house mouse, Mus musculus subsp. domesticus. The isolates are highly related form separate clade genus, as judged by tree analyses using either multi-locus sequence...
Multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) family transporters are essential in glycan synthesis, flipping lipid-linked precursors across cell membranes. Yet, how they select their substrates remains enigmatic. Here, we investigate the substrate specificity of MOP capsular polysaccharide (CPS) synthesis pathway Streptococcus pneumoniae . These capsule flippases collectively transport more than 100 types precursors. To determine whether can substitute for one another, developed a...
Microbial communities are fundamental to life on Earth. Different strains within these often connected by a highly metabolic network, where the growth of one strain depends activities other community members. While distributed functions allow microbes reduce costs and optimize pathways, they make them metabolically dependent. Here, we hypothesize that such dependencies can be detrimental in situations external conditions change rapidly, as do natural environments. After shift conditions,...
Human-adapted Neisseria includes two pathogens, gonorrhoeae and meningitidis, at least 13 species of commensals that colonize many the same niches as pathogens. The Type IV pilus plays an important role in biology pathogenic Neisseria. In these species, Sigma factor RpoD (σ(70)), Integration Host Factor, repressors RegF CrgA regulate transcription pilE, gene encoding structural subunit. is also a strictly conserved trait commensal We present evidence different mechanism regulates pilE...
ABSTRACT The øX174 DNA pilot protein H forms an oligomeric DNA-translocating tube during penetration. However, monomers are incorporated into 12 pentameric assembly intermediates, which become the capsid's icosahedral vertices. protein's N terminus, a predicted transmembrane helix, is not represented in crystal structure. To investigate its functions, series of absolute and conditional lethal mutations were generated. proteins, deletion triple substitution, efficiently virus-like particles...
Summary Post‐translational acetylation is a common protein modification in bacteria. It was recently reported that Neisseria gonorrhoeae acetylates the Type IV pilus retraction motor, PilT. Here, we show recombinant PilT can be acetylated vitro and does not affect ultrastructure. To investigate function of acetylation, mutated an lysine, K117, to mimic its or unacetylated forms. These mutations were tolerated by wild‐type N. gonorrhoeae, but they carrying inducible pilE when grown without...
Abstract Secreted immunoglobulins, predominantly SIgA, influence the colonization and pathogenicity of mucosal bacteria. While part this effect can be explained by SIgA-mediated bacterial aggregation, we have an incomplete picture how SIgA binding influences cells independently aggregation. Here show that akin to microscale crosslinking cells, targeting Salmonella Typhimurium O-antigen extensively crosslinks O-antigens on surface individual at nanoscale. This results in essentially...
Introductory paragraph The ability of gut bacterial pathogens to escape immunity by antigenic variation, particularly via changes surface-exposed antigens, is a major barrier immune clearance 1 . However, not all variants are equally fit in environments 2, 3 It should therefore be possible exploit such mechanisms direct an evolutionary trade-off. Here we demonstrated this phenomenon using Salmonella enterica subspecies serovar Typhimurium ( S. Tm). A dominant surface antigen Tm its...
Abstract Salmonella spp. express pathogenicity island 1 (SPI-1) genes to mediate the initial phase of interaction with host cells. Prior studies indicate short-chain fatty acids, microbial metabolites at high concentrations in gastrointestinal tract, limit SPI-1 gene expression. A number reports show only a subset cells population these genes, suggesting acids could decrease population-level expression by acting on per-cell and/or proportion expressing Here, we combine single-cell,...