A5008266377- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Adenosine and Purinergic Signaling
- Photoreceptor and optogenetics research
- Acupuncture Treatment Research Studies
- Neurobiology and Insect Physiology Research
- Cannabis and Cannabinoid Research
- Multiple Sclerosis Research Studies
- Protein Structure and Dynamics
- Ion channel regulation and function
- Nerve injury and regeneration
- Receptor Mechanisms and Signaling
- Neuroscience of respiration and sleep
- Plant and Biological Electrophysiology Studies
- Vagus Nerve Stimulation Research
- Complementary and Alternative Medicine Studies
- Planarian Biology and Electrostimulation
- Gene Regulatory Network Analysis
- Neural dynamics and brain function
- Pain Management and Placebo Effect
- Cellular Mechanics and Interactions
- Neonatal and fetal brain pathology
- Microbial Metabolic Engineering and Bioproduction
- Neurological Complications and Syndromes
University of the Basque Country
2014-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2014-2024
Achucarro Basque Center for Neuroscience
2014-2024
The University of Texas Medical Branch at Galveston
2020
Universidad Nacional Autónoma de México
2015-2020
Centro de Investigación Biomédica en Red
2015-2017
Euskadiko Parke Teknologikoa
2016
Instituto de Salud Carlos III
2013
Oligodendrocyte death and demyelination are hallmarks of multiple sclerosis (MS). Here we show that ATP signaling can trigger oligodendrocyte excitotoxicity via activation calcium-permeable P2X 7 purinergic receptors expressed by these cells. Sustained in vivo causes lesions reminiscent the major features MS plaques, i.e., demyelination, death, axonal damage. In addition, treatment with antagonists chronic experimental autoimmune encephalomyelitis (EAE), a model MS, reduces ameliorates...
Phagocytosis is essential to maintain tissue homeostasis in a large number of inflammatory and autoimmune diseases, but its role the diseased brain poorly explored. Recent findings suggest that adult hippocampal neurogenic niche, where excess newborn cells undergo apoptosis physiological conditions, phagocytosis efficiently executed by surveillant, ramified microglia. To test whether microglia are efficient phagocytes as well, we confronted them with series apoptotic challenges discovered...
Microglia survey the brain microenvironment for signals of injury or infection and are essential initiation resolution pathogen- tissue damage-induced inflammation. Understanding mechanism microglia responses during pathology is hence vital to promote regenerative responses. Here, we analyzed role purinergic receptor P2X4 (P2X4R) in microglia/macrophages autoimmune Blockade P2X4R signaling exacerbated clinical signs experimental encephalomyelitis (EAE) model also favored activation a...
Brain ischemia leading to stroke is a major cause of disability in developed countries. Therapeutic strategies have most commonly focused on protecting neurons from ischemic damage. However, damage white matter causes oligodendrocyte death, myelin disruption, and axon dysfunction, it partially mediated by glutamate excitotoxicity. We previously demonstrated that oligodendrocytes express ionotropic purinergic receptors. The objective this study was investigate the role signaling ischemia....
During brain ischemia, an excessive release of glutamate triggers neuronal death through the overactivation NMDA receptors (NMDARs); however, underlying pathways that alter homeostasis and whether synaptic or extrasynaptic sites are responsible for excess remain controversial. Here, we monitored ischemia-gated currents in pyramidal cortical neurons slices from rodents response to oxygen glucose deprivation (OGD) as a real-time sensor identify source determined extent damage. Blockade...
The endocannabinoids 2-araquidonoylglycerol (2-AG) and anandamide (AEA) are bioactive lipids crucially involved in the regulation of brain function basal pathological conditions. Blockade endocannabinoid metabolism has emerged as a promising therapeutic strategy for inflammatory diseases central nervous system, including myelin disorders such multiple sclerosis. Nevertheless, biological actions degradation inhibitors oligodendrocytes white matter tracts still ill defined. Here we show that...
Abstract Glutamate uptake is crucial to terminate glutamate signaling and prevent excitotoxicity. The present study describes the expression of functional transporters GLAST GLT‐1 in oligodendrocytes by means electrophysiology, assays, immunocytochemistry. Inhibition uptake, both oligodendrocyte cultures isolated optic nerves, increases levels causes excitotoxicity, which prevented alpha‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid (AMPA) kainate receptor antagonists. Furthermore,...
Glutamate excitotoxicity and complement attack have both been implicated separately in the generation of tissue damage multiple sclerosis its animal model, experimental autoimmune encephalomyelitis. Here, we investigated whether glutamate receptor activation sensitizes oligodendrocytes to attack. We found that a brief incubation with followed by exposure was lethal vitro freshly isolated optic nerves. Complement toxicity induced kainate but not AMPA receptors abolished removing calcium from...
The role of P2X7 receptors and pannexin-1 channels in ischemic damage remains controversial. Here, we analyzed their contribution to postanoxic depolarization after ischemia cultured neurons brain slices. We observed that pharmacological blockade or delayed the onset currents reduced slope, simultaneous inhibition did not further enhance effects blocking either one. These results were confirmed acute cortical slices from knockout mice. Oxygen-glucose deprivation organotypic cultures caused...
Pannexin1 (Panx1) is a plasma membrane channel permeable to relatively large molecules, such as ATP. In the central nervous system (CNS) Panx1 found in neurons and glia immune macrophages T-cells. We tested hypothesis that Panx1-mediated ATP release contributes expression of Experimental Autoimmune Encephalomyelitis (EAE), an animal model for multiple sclerosis, using wild-type (WT) knockout (KO) mice. KO mice displayed delayed onset clinical signs EAE decreased mortality compared WT mice,...
Myelination requires oligodendrocyte-neuron communication, and both neurotransmitters contact interactions are essential for this process. Oligodendrocytes endowed with neurotransmitter receptors whose expression levels properties may change during myelination. However, only scant information is available about the extent timing of these changes or how they regulated by interactions. Here, we used electrophysiology to study ionotropic GABA, glutamate, ATP in oligodendrocytes derived from...
Associative memory is the main type of learning by which complex organisms endowed with evolved nervous systems respond efficiently to certain environmental stimuli. It has been found in different multicellular species, from cephalopods humans, but never individual cells. Here we describe a motility pattern consistent associative conditioned behavior microorganism Amoeba proteus. We use controlled direct-current electric field as stimulus, and specific chemotactic peptide unconditioned...
Abnormalities in myelination are associated to behavioral and cognitive dysfunction neurodevelopmental psychiatric disorders. Thus, therapies promote or accelerate could potentially ameliorate symptoms autism. Clemastine, a histamine H1 antagonist with anticholinergic properties against muscarinic M1 receptor, is the most promising drug promyelinating properties. Clemastine penetrates blood brain barrier efficiently promotes remyelination different animal models of neurodegeneration...
Adenosine receptor activation is involved in myelination and apoptotic pathways linked to neurodegenerative diseases. In this study, we investigated the effects of adenosine viability oligodendrocytes rat optic nerve. Selective A 3 receptors pure cultures caused concentration‐dependent necrotic death which was preceded by oxidative stress mitochondrial membrane depolarization. Oligodendrocyte apoptosis induced caspase‐dependent caspase‐independent. addition dissociated cultures, incubation...