A5074124976- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Chemical and Physical Properties in Aqueous Solutions
- Viral gastroenteritis research and epidemiology
- Additive Manufacturing and 3D Printing Technologies
- Microfluidic and Bio-sensing Technologies
- Bacteriophages and microbial interactions
- Spectroscopy and Chemometric Analyses
- Chromatography in Natural Products
- Microbial Metabolic Engineering and Bioproduction
- Virus-based gene therapy research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- Hydrogels: synthesis, properties, applications
- Proteins in Food Systems
- Advanced Biosensing Techniques and Applications
Karlsruhe Institute of Technology
2016-2025
Institute of Process Engineering
2014-2023
Max Planck Institute of Colloids and Interfaces
2022
Biolog Life Science Institute
2022
Biocat
2021
John Wiley & Sons (United States)
2015-2019
Hudson Institute
2016-2019
Institute of Engineering
2017
Bioengineering Center
2015-2017
National Center for Genetic Engineering and Biotechnology
2015-2017
Abstract The necessity of being “first on the market” paired with need to cut production costs highlights for strategies and techniques allowing a fast process development while at same time covering high number potential parameters in order reach an optimal sequence. This can only be met by use throughput screening (HTS) techniques. In this paper HTS applications presently applied bioprocess are reviewed, their chromatographic parameter evaluation is discussed concepts rational design...
Abstract This study evaluates the applicability of liquid handling workstation Tecan Evo Freedom 200 and μL Media Scout RoboColumns to dynamic chromatographic operations such as frontal analysis (breakthrough) elution experiments in a high throughput screening mode. Breakthrough were conducted using BSA model protein stationary phases Poros 50 D, Q Ceramic HyperD DEAE Sepharose FF. The obtained capacities at 10 % breakthrough matched well with reference data. Elution performed applying three...
Aqueous two-phase systems (ATPSs) as separation technique have regained substantial interest from the biotech industry. Biopharmaceutical companies faced with increasing product titers and stiffening economic competition reconsider ATPS an alternative to chromatography. As implementation of is material, time, labor intensive, a miniaturized automated screening process would be beneficial. In this article such method, its statistical evaluation, application biopharmaceutical task are shown....
The biopharmaceutical industry is at a turning point moving toward more customized and patient-oriented medicine (precision medicine). Straightforward routines such as the antibody platform process are extended to production processes for new portfolio of molecules. As consequence, individual tailored productions require generic approaches fast dedicated purification development. In this article, different effective strategies in development reviewed that can analogously be used generation...
Abstract Mechanistic modeling of chromatography processes is one the most promising techniques for digitalization biopharmaceutical process development. Possible applications models range from in silico optimization early phase development to root cause investigation during manufacturing. Nonetheless, cumbersome and complex model calibration still decelerates implementation mechanistic industry. Therefore, industry demands strategies that ensure adequate certainty a limited amount time. This...
Multimodal chromatography offers an increased selectivity compared to unimodal chromatographic methods and is often employed for challenging separation tasks in industrial downstream processing (DSP). Unfortunately, the implementation of multimodal polishing into a generic platform can be hampered by non-robust conditions leading time cost intensive process development. Mechanistic modeling assist experimental development but readily applicable easy calibrate models are lacking. In this...
Multimodal chromatography has emerged as a powerful method for the purification of therapeutic antibodies. However, process development this separation technique remains challenging because an intricate and molecule-specific interaction towards multimodal ligands, leading to time-consuming costly experimental optimization. This study presents multiscale modeling approach predict chromatographic behavior antibodies based on their sequence information. Linear gradient elution (LGE) experiments...
Abstract A feasibility study to couple high throughput screening of packed bed chromatography with mass spectrometric detection by SELDI‐TOF MS is presented. As model system monoclonal antibodies (mAb) versus host cell protein (HCP) from an industrial cultivation was chosen. Packed screened on a TECAN Evo Freedom 200 station using miniaturized chromatographic columns placed specially designed array carrier linked commercially available T‐Stack module. Gradient elution the bound proteins...
Molecular Dynamics (MD) simulations are a promising tool to generate molecular understanding of processes related the purification proteins. Polyethylene glycols (PEG) various length commonly used in production and The mechanisms behind PEG driven precipitation, aqueous two-phase formation or effects PEGylation however still poorly understood.In this paper, we ran MD single molecules variable explicitly simulated water. resulting structures good agreement with experimentally determined 3D...
Abstract In the last decade, high‐throughput downstream process development techniques have entered biopharmaceutical industry. As chromatography is standard purification method, several chromatographic methods been developed and applied including miniaturized columns for utilization on liquid handling stations. These were used to setup a complete station first time. this article, monoclonal antibody was established in lab‐scale afterwards. The scale‐down methodology presented discussed....