A5057398382- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Actinomycetales infections and treatment
- Insect symbiosis and bacterial influences
- Bacterial biofilms and quorum sensing
- Insect and Arachnid Ecology and Behavior
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Insect and Pesticide Research
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Biofuel production and bioconversion
- Protist diversity and phylogeny
- Vibrio bacteria research studies
- Plant Pathogenic Bacteria Studies
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Mycobacterium research and diagnosis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Probiotics and Fermented Foods
- Oral microbiology and periodontitis research
- Enzyme Production and Characterization
- Identification and Quantification in Food
- Molecular Biology Techniques and Applications
University of Malaya
2010-2022
Phylogenetic analysis based on 16S rRNA gene sequences of the genus Streptomyces showed presence six distinguishable clusters, with 100 % sequence similarity values among strains in each cluster; thus they shared almost same evolutionary distance. This result corroborated well outcome core (orthologous clusters) genome phylogeny 190 genomes including species those clusters. These preeminent results led to an investigation genome-based indices such as digital DNA-DNA hybridization (dDDH),...
The weaver ant Oecophylla smaragdina is an aggressive predator of other arthropods and has been employed as a biological control agent against many insect pests in plantations. Despite playing important roles pest management, information about the microbiota O. limited. In this work, number colonies (n = 12) from Malaysia had studied on their microbiome profile using Illumina 16S rRNA gene amplicon sequencing. We characterized core associated with these investigated variation between...
The family Streptomycetaceae is a large and diverse within the phylum Actinomycetota . members of are known for their ability to produce medically important secondary metabolites, notably antibiotics. In this study, 19 type strains showing low 16S rRNA gene similarity (<97.3 %) other were identified high genetic diversity was reflected in phylogenomic analysis using conserved universal proteins. This resulted identification six distinct genus-level clades, with two separated from genus...
N-acylhomoserine lactones (AHLs) are well-studied signalling molecules produced by some Gram-negative Proteobacteria for bacterial cell-to-cell communication or quorum sensing. We have previously demonstrated the degradation of AHLs an Antarctic bacterium, Planococcus versutus L10.15T, at low temperature through production AHL lactonase. In this study, we cloned lactonase gene and characterized purified novel enzyme. Rapid resolution liquid chromatography analysis indicated that AidP...
A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15T. The organism isolated from soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using quorum-quenching enrichment medium. Growth occurred at 4-30 °C, pH 6-11 and moderately high salinity (0-15 %, w/v, NaCl), with optimal growth 26 7-8 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that L10.15T...
Abstract Planococcus is a Gram-positive halotolerant bacterial genus in the phylum Firmicutes, commonly found various habitats Antarctica. Quorum quenching (QQ) disruption of cell-to-cell communication (known as quorum sensing), which has previously been described mesophilic bacteria. This study demonstrated QQ activity psychrotolerant strain, versutus strain L10.15 T , isolated from soil sample obtained near an elephant seal wallow Whole genome analysis this revealed presence N -acyl...
Pandoraea species have been isolated from diverse environmental samples and are emerging important respiratory pathogens, particularly in people with cystic fibrosis (CF). In the present study, two bacterial isolates initially recovered consecutive sputum collected a CF patient identified as pnomenusa underwent polyphasic taxonomic analysis. The were found to be Gram-negative, facultative anaerobic motile bacilli subsequently designated strains 6399T (=LMG29626T=DSM103228T) 7641...
Abstract Arthrobacter alpinus R3.8 is a psychrotolerant bacterial strain isolated from soil sample obtained at Rothera Point, Adelaide Island, close to the Antarctic Peninsula. Strain was sequenced in order help discover potential cold active enzymes with biotechnological applications. Genome analysis identified various adaptation genes including some coding for anti-freeze proteins and cold-shock proteins, involved bioremediation of xenobiotic compounds naphthalene, chitinolytic N...
A Gram-stain-negative, aerobic, rod-shaped, leaf-associated bacterium, designated JS23T, was isolated from surface-sterilized leaf tissue of an oil palm grown in Singapore and investigated by polyphasic taxonomy. Phylogenetic analyses based on 16S rRNA gene sequences 180 conserved genes the genome several members Burkholderiaceae revealed that strain JS23T formed a distinct evolutionary lineage independent other taxa within family Burkholderiaceae. The predominant ubiquinone Q-8. primary...
Abstract A bacterial strain designated as P08 T was isolated from laboratory tap water during a quality assessment in University of Malaya, Malaysia. The Gram‐negative, rod‐shaped, nonmotile, and aerobic bacterium. Complete genome comprised 2,820,660 bp chromosome with G + C content 36.43%. Both 16S rRNA phylogeny phylogenetic tree inferred the core gene matrix demonstrated that formed hitherto unknown subline within family Neisseriaceae . Ortho average nucleotide identity (OrthoANI) values...
Root exudates are chemical compounds that released from living plant roots and provide significant energy, carbon, nitrogen phosphorus sources for microbes inhabiting the rhizosphere. The shape microflora associated with plant, as well influences health productivity. Therefore, a better understanding of trophic link is established between bacteria necessary. In this study, comprehensive survey on utilization grapevine rootstock related organic acids were conducted vineyard soil isolate which...
The most common quorum sensing (QS) system in Gram-negative bacteria consists of signaling molecules called N-acyl-homoserine lactones (AHLs), which are synthesized by an enzyme AHL synthase (LuxI) and detected a transcriptional regulator (LuxR) that usually located close proximity. However, many recent studies have also evidenced the presence LuxR solos LuxR-related proteins Proteobacteria devoid cognate LuxI synthase. Pandoraea species opportunistic pathogens frequently isolated from...
DATA REPORT article Front. Microbiol., 31 August 2016Sec. Microbial Physiology and Metabolism Volume 7 - 2016 | https://doi.org/10.3389/fmicb.2016.01362
Abstract In this study, bacterial strains Ha5 T , Ta1 and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina . They distinguished as based on matrix-assisted laser desorption ionization-time flight (MALDI-TOF) mass spectrometry distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells these Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive oxidase-negative. able to grow at 15–37°C (optimum, 28–30°C) in the presence 0–1.5 % (w/v) NaCl...
Abstract Term heterotypic synonym refers to different names have been associated with type strains, however from the opinion of a bacteriologist, belongs same taxon and term subspecies strains genetically close organisms that were diverging phenotypically. In this study, sequenced publicly available genomes in Edgar 2.0 server carefully analysed based on high (>98 %) amino acid identity value, synonyms putatively identified. The 16S rRNA gene sequence those species used for construction...