Abstract Some populations of marine organisms appear to have inherent tolerance or the capacity for acclimation stressful environmental conditions, including those associated with climate change. Sydney rock oysters from B2 breeding line exhibit resilience ocean acidification ( OA ) at physiological level. To understand molecular basis this resilience, we analysed gill transcriptome that had been exposed near‐future projected pH over two consecutive generations. Our results suggest...

Marine organisms need to adapt in order cope with the adverse effects of ocean acidification and warming. Transgenerational exposure CO2 stress has been shown enhance resilience offspring from a number species. However, molecular basis underlying such adaptive responses is currently unknown. Here, we compared transcriptional profiles two genetically distinct oyster breeding lines following transgenerational elevated explore acclimation or adaptation these organisms. The expression key target...

Currently, there are few predictive biomarkers in key biomonitoring species, such as oysters, that can detect heavy metal pollution coastal waterways. Several attributes make oysters superior to other organisms for positive of pollution. In particular, they filter feeders with a high capacity bioaccumulation. this study, we used two proteomics approaches, namely label‐free shotgun based on SDS‐PAGE gel separation and gas phase fractionation, investigate the stress responses Sydney rock...

Abstract Previous work suggests that larvae from Sydney rock oysters have been selectively bred for fast growth and disease resistance are more resilient to the impacts of ocean acidification than nonselected, wild‐type oysters. In this study, we used proteomics investigate molecular differences between oyster populations in adult identify whether these form basis observations seen larvae. Adult a selective breeding line (B2) nonselected wild types ( WT ) were exposed 4 weeks elevated p CO 2...

This study characterises the molecular processes altered by both elevated CO2 and increasing temperature in oysters. Differences resilience of marine organisms against environmental stressors associated with climate change will have significant implications for sustainability coastal ecosystems worldwide. Some evidence suggests that can differ between populations within a species. B2 oysters represent unique genetic resource because their capacity to better withstand impacts at physiological...

The acidification of oceans is predicted to fundamentally alter marine ecosystems. Previous studies have found that elevated CO2 has an effect on adult calcification, fertilisation and larval development, perhaps because the organisms' inability regulate acid-base status, but little known about mechanisms underlie such responses. This study investigated growth response larvae a wild selectively bred line Sydney rock oyster, Saccostrea glomerata, measured pattern expression proteins. Overall...

Runoff from acid sulfate soils (ASS) has severe environmental and economic impacts on estuarine ecosystems. Oysters display reduced abundance, growth rate shell thickness when exposed to ASS runoff, yet the molecular underpinnings of their responses have not been explored. We hypothesised that proteomes wild Sydney rock oysters, Saccostrea glomerata, would differ between populations recurrently compared with those unaffected by runoff ASS. used two-dimensional electrophoresis compare protein...

In the current study, we tested effects of common environmental contaminants (the metals zinc and lead) on gene expression in Sydney rock oysters (Saccrostrea glomerata). Oysters were exposed to a range metal concentrations under controlled laboratory conditions. The 14 putative stress response genes was then measured using quantitative, real-time (q) PCR. all significantly affected (p < 0.05 vs. nonexposed controls) by at least one metals, dose metal. For 5 target (actin, calmodulin,...

This study tested the proteomic responses of three spatially distinct Sydney rock oyster populations to elevated pCO2. Oysters were collected from environmentally different sites, two chronically affected by acid sulfate soil. each exposed ambient (380µatm) or (856 and 1500µatm) pCO2 for 4 weeks. Subsequent analysis haemolymph revealed that (1) there differences between proteomes after exposure pCO2, (2) mounted significantly Proteins differed in concentration treatments fell into five broad...

Emissions of ammonia and methane from an anaerobic lagoon at a swine animal feeding operation were evaluatedfive times over period two years. The plane-integrated (PI) open-path Fourier transform infrared spectrometry(OP-FTIR) methodology was used to transect the plume five locations. path-integrated concentration data, along withwind speed direction analyzed using emission flux computational method known as Vertical Radial PlumeMapping (VRPM). VRPM algorithm utilizes smooth basis function...

Bivalve molluscs, such as oysters, are threatened by shifts in seawater chemistry resulting from climate change. However, a few species and populations within stand out for their capacity to cope with the impacts of change-associated stressors. Understanding intracellular basis differential responses can contribute development strategies minimise pervasive effects changing ocean on marine organisms. In this study, we explored acidification two genetically distinct Sydney rock oysters...