A5018700861- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Biochemical and Molecular Research
- Glycosylation and Glycoproteins Research
- Synthesis and Biological Evaluation
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Allergic Rhinitis and Sensitization
- Sphingolipid Metabolism and Signaling
- Toxoplasma gondii Research Studies
- Parasites and Host Interactions
- Bacteriophages and microbial interactions
- Food Allergy and Anaphylaxis Research
- Herpesvirus Infections and Treatments
- Polyamine Metabolism and Applications
- Rabies epidemiology and control
- Peptidase Inhibition and Analysis
- Malaria Research and Control
- Toxin Mechanisms and Immunotoxins
- Protist diversity and phylogeny
- Protease and Inhibitor Mechanisms
- Aldose Reductase and Taurine
- Pharmacological Effects of Natural Compounds
- Herbal Medicine Research Studies
- Parasitic Diseases Research and Treatment
Administración Nacional de Laboratorios e Institutos de Salud
2012-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
1992-2023
Ministerio de Ciencia, Tecnología e Innovación
2022
Ministerio de Salud
2008-2021
Ministerio de Salud
2015
National University of General San Martín
2000-2003
University of Buenos Aires
1992
Fundación Ciencias Exactas y Naturales
1991
Trypanosoma cruzi , the parasitic protozoan that causes Chagas disease, contains a major cysteine proteinase, cruzipain. This lysosomal enzyme bears an unusual C‐terminal extension number of post‐translational modifications, and most antibodies in natural experimental infections are directed against it. In this report we took advantage UV‐MALDI‐TOF mass spectrometry conjunction with peptide N‐glycosidase F deglycosylation high performance anion exchange chromatography analysis to address...
The subcellular localization of NAD- and NADP-linked glutamate dehydrogenases (GDH-NAD GDH-NADP), alanine aminotransferase (ALAT) aspartate (ASAT) in epimastigotes Trypanosoma cruzi was studied by digitonin extraction from whole cells, fractionation differential centrifugation. A isopycnic ultracentrifugation. All enzymes presented both a cytosolic mitochondrial form; addition, GDH-NADP seems to have third, still undefined, localization. results are compatible with the existence two pathways...
Trypanosoma cruzi, the agent of Chagas disease contains a major cysteine proteinase, cruzipain (Cz), with an unusual carboxyl-terminal extension (C-T). We have previously reported presence sulfate groups in N-linked oligosaccharide chains this domain. In order to evaluate immune responses sulfated moieties on Cz, BALB/c mice were immunized purified Cz and C-T prior after desulfation treatment. The humoral response sulfates or was mainly IgG2b. Interestingly, abolishment IgG2b reactivity when...
Protein expression, characterized in Western blots and gelatinolytic activity, of cruzipain (Cr), the major Trypanosoma cruzi cysteine proteinase, was compared among 3 attenuated T. strains (TUL 0, TCC, Y null) their virulent counterparts 2, Tulahuen, Y). All displayed a weaker activity as with counterparts. The electrophoretic mobility immunological reactivity revealed quantitative qualitative differences, parasites showing bands less density all lower 2 them, strains. Sequence analysis 1...
With the aim to study proteinases released culture medium during Trypanosoma cruzi metacyclogenesis, presence of cysteine (CPs) was analysed in supernatants obtained throughout differentiation induced by stimulation epimastigotes with Triatoma infestans hindgut homogenate. In SDS-gelatin containing gels, an important endopeptidase activity apparent molecular weight range between 97 and 116 kDa encountered at pH 6, which abolished specific proteinase inhibitor E-64 TLCK, but not pepstatin,...
It is well known that allergen extracts used for specific therapy of allergic disorders are commonly stored as mixtures, causing an alteration its stability.The aim this report to identify pollen allergens susceptible degradation during storage mixtures containing different sources proteases in the absence glycerol a preserving agent.Mixes Lolium perenne (Lol p) extract with either Aspergillus fumigatus or Periplaneta americana were prepared and co-incubated 90 days at 4 degrees C. Samples...
Single units of O-linked N-acetylglucosamine (GlcNAc), usually components nuclear and cytoplasmatic proteins, are present at the C-terminal domain cruzipain (Cz), a lysosomal major antigen from Trypanosoma cruzi. On other hand, antibodies directed against some self-antigens like myosin associated with Chagas heart disease. The participation O-GlcNAc moieties in molecular antigenicity Cz was determined using GlcNAc linked to aprotinin by ELISA. immune cross-reactivity between is mainly...
Cruzipain (Cz), the major cysteine proteinase of Trypanosoma cruzi , is a glycoprotein that contains sulfated high‐mannose‐type oligosaccharides. We have previously determined these sulfate groups are targets specific immune responses. In order to evaluate structural requirements for antibody recognition Cz, systematic structure–activity study chemical characteristics needed binding Cz epitope was performed by immunoassays. With this aim, different synthesized molecules were coupled proteins...
Cell-free extracts of epimastigotes Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the latter procedure also allowed complete separation pHPLDH. subcellular localization both T. enzymes, as determined digitonin extraction, fractionation differential centrifugation, isopycnic ultracentrifugation in...
<i>Background:</i> It is important to study the crossreactivity between orange tree pollen (OTPE) and fruit (OFE) due high incidence of pollen/food-related allergies worldwide. The aim present was determine antigenic relationship OTPE OFE. <i>Methods:</i> OFErabbit antisera as well sera from patients allergic OFE were comparatively applied in IgG- and/or IgE-specific ELISA inhibition, crossover or inhibition immunoblotting assays using allergenic extracts solid phase....