The functions of POU class 5 transcription factor 1 (Oct-4) and caudal-type homeobox 2 (Cdx2) in the differentiation murine inner cell mass (ICM) trophectoderm (TE) have been described detail. However, little is known about roles OCT-4 CDX2 preimplantation bovine embryos. To elucidate their during early development embryos, we performed downregulation using RNA interference. We injected OCT-4- or CDX2-specific short interfering RNAs (siRNAs) into zygotes. rate blastocyst OCT-4-downregulated...

Transcription factor TEA domain family transcription 4 (Tead4) is one of the key factors involved in differentiation trophectoderm (TE) murine embryos. However, knowledge on roles TEAD4 preimplantation development during bovine embryos currently limited. This study examined transcript and protein expression patterns attempted to elucidate functions using RNA interference. mRNA was found be upregulated between 16-cell morula stages, nuclear localization detected at stage, as well subsequent...

Oct-4, a member of the POU family transcription factors, is key factor that regulates segregation inner cell mass (ICM) and trophectoderm (TE) during transition from morula to blastocyst in mice. However, little known about its role porcine early embryogenesis. To determine function OCT-4 ICM TE embryos, we studied developmental morphology embryos using RNA interference technology. Our experiments demonstrated when 1-cell stage were co-injected with small interfering (siRNA)for targeted...

Krüppel-like protein Gli-similar 1 (GLIS1) is known as a direct reprogramming factor for the generation of induced pluripotent stem cells. The objective this study was to investigate role GLIS1 in preimplantation development bovine embryos. transcripts vitro-matured oocytes and 1-cell 4-cell stage embryos were detected, but they either absent or at trace levels 8-cell blastocyst stages. We attempted downregulation early by RNA interference evaluated developmental competency gene transcripts,...

TEA domain family transcription factor 4 (Tead4) is known to be important for the trophectoderm (TE) segregation in murine embryos. However, role of TEAD4 early development porcine embryos still unknown. We examined expression patterns and attempted determine functions during preimplantation using RNA interference. mRNA was upregulated from 2-4-cell 8-16-cell stages then decreased blastocyst stage. Nuclear localization protein detected at 16-cell stage, as well subsequent developmental...

In mouse development, differentiation of the inner cell mass (ICM) and trophectoderm (TE) during transition from morula to blastocyst stage is regulated by Hippo pathway; however, functions pathway in porcine embryogenesis have not been investigated. present study, we examined gene expression patterns members yes-associated protein 1 (YAP1) large tumor suppressor 2 (LATS2) these genes preimplantation development using RNA interference. Both YAP1 LATS2 mRNA levels were shown high vitro...

The evolutionary introduction of asymmetric cell division (ACD) into the developmental program facilitates formation a new type, contributing to diversity and, eventually, species diversification. micromere sea urchin embryo may serve as one those examples: An ACD at 16-cell stage forms micromeres unique echinoids among echinoderms. We previously reported that polarity factor, Activator G-protein Signaling (AGS), plays crucial role in formation. However, AGS and its associated factors are...

The evolutionary introduction of asymmetric cell division (ACD) into the developmental program facilitates formation a new type, contributing to diversity and, eventually, species diversification. micromere sea urchin embryo may serve as one those examples: an ACD at 16-cell stage forms micromeres unique echinoids among echinoderms. We previously reported that polarity factor, activator G-protein signaling (AGS), plays crucial role in formation. However, AGS and its associated factors are...

The evolutionary introduction of asymmetric cell division (ACD) into the developmental program facilitates formation a new type, contributing to diversity and, eventually, species diversification. micromere sea urchin embryo may serve as one those examples: An ACD at 16-cell stage forms micromeres unique echinoids among echinoderms. We previously reported that polarity factor, Activator G-protein Signaling (AGS), plays crucial role in formation. However, AGS and its associated factors are...

The evolutionary introduction of asymmetric cell division (ACD) into the developmental program facilitates formation a new type, contributing to diversity and, eventually, species diversification. micromere sea urchin embryo may serve as one those examples: An ACD at 16-cell stage forms micromeres unique echinoids among echinoderms. We previously reported that polarity factor, Activator G-protein Signaling (AGS), plays crucial role in formation. However, AGS and its associated factors are...

The evolutionary introduction of asymmetric cell division (ACD) into the developmental program facilitates formation a new type, contributing to diversity and, eventually, species diversification. micromere sea urchin embryo may serve as one those examples: an ACD at 16-cell stage forms micromeres unique echinoids among echinoderms. We previously reported that polarity factor, activator G-protein signaling (AGS), plays crucial role in formation. However, AGS and its associated factors are...

Steroidogenic Factor 1 (SF-1/NR5A1), an orphan nuclear receptor, is important for sexual differentiation and the development of multiple endocrine organs, as well cell proliferation in cancer cells. Activating transcription factor 3 (ATF3) a transcriptional repressor, its expression rapidly induced by DNA damage oncogenic stimuli. Since both NR5A1 ATF3 can regulate cooperate with several factors, we hypothesized that may interact plays functional role development. First, found physically...