A5070288104- Plant pathogens and resistance mechanisms
- RNA and protein synthesis mechanisms
- Plant-Microbe Interactions and Immunity
- RNA modifications and cancer
- Fungal and yeast genetics research
- Plant and Fungal Interactions Research
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Agronomic Practices and Intercropping Systems
- Forest Insect Ecology and Management
- Microbial Metabolic Engineering and Bioproduction
- Cystic Fibrosis Research Advances
- Microtubule and mitosis dynamics
- 14-3-3 protein interactions
- Plant Disease Management Techniques
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Biofuel production and bioconversion
- Pancreatic function and diabetes
- Advanced biosensing and bioanalysis techniques
Huazhong Agricultural University
2015-2025
Shanghai Zhangjiang Laboratory
2024
The University of Texas Southwestern Medical Center
2020-2021
Purdue University West Lafayette
2015
Small, secreted proteins have been found to play crucial roles in interactions between biotrophic/hemi-biotrophic pathogens and plants. However, little is known about the of these produced by broad host-range necrotrophic phytopathogens during infection. Here, we report that a cysteine-rich, small protein SsSSVP1 phytopathogen Sclerotinia sclerotiorum was experimentally confirmed be protein, secretion from hyphae followed internalization cell-to-cell movement independent pathogen host cells....
Abstract Our comparative genomic analysis showed that the numbers of plant cell wall (PCW)- and fungal (FCW)-degradation-associated carbohydrate-active enzymes (CAZymes) in necrotrophic hemibiotrophic fungi are significantly larger than most biotrophic fungi. However, our transcriptional analyses CAZyme-encoding genes Melampsora larici-populina , Puccinia graminis Sclerotinia sclerotiorum many encoding PCW- FCW-degradation-associated CAZymes were up-regulated during infection both fungi,...
Abstract Essential cellular functions require efficient production of many large proteins but synthesis encounters obstacles in cells. Translational control is mostly known to be regulated at the initiation step. Whether translation elongation process can feedback regulate efficiency unclear. Codon usage bias, a universal feature all genomes, plays an important role determining gene expression levels. Here, we discovered that there conserved codon usage-dependent genome-wide negative...
Eukaryotic cell cycle involves a number of protein kinases important for the onset and progression through mitosis, most which are well characterized in budding fission yeasts conserved other fungi. However, unlike model yeast filamentous fungi that have single Cdc2 essential progression, wheat scab fungus Fusarium graminearum contains two CDC2 orthologs. The cdc2A cdc2B mutants had no obvious defects growth rate conidiation but deletion both them is lethal, indicating these orthologs...
Under amino acid starvation conditions, eukaryotic organisms activate a general control response. In Neurospora crassa, Cross Pathway Control Protein 1 (CPC-1), the ortholog of Saccharomyces cerevisiae bZIP transcription factor GCN4, functions as master regulator Codon usage biases are universal feature genomes and critical for regulation gene expression. Although codon has also been implicated in protein structure function, genetic evidence supporting this conclusion is very limited. Here,...
Codon usage bias is a universal feature of all genomes and plays an important role in regulating protein expression levels. Modification adenosine to inosine at the tRNA anticodon wobble position (I34) by deaminases (ADATs) observed eukaryotes has been proposed explain correlation between codon pool. However, how pool affected I34 modification influence usage-dependent gene unclear. Using Neurospora crassa as model system, combining molecular, biochemical bioinformatics analyses, we show...
Microbial opsins play a crucial role in responses to various environmental signals. Here, we report that the microbial opsin homolog gene sop1 from necrotrophic phytopathogenic fungus Sclerotinia sclerotiorum was dramatically up-regulated during infection and sclerotial development compared with vegetative growth stage. Further, study showed essential for growth, full virulence of S. sclerotiorum. Sop1-silenced transformants were more sensitive high salt stress, fungicides osmotic stress....
Codon usage bias (CUB) could reflect co-evolutionary changes between viruses and hosts in contrast to plant animal viruses, the systematic analysis of codon among mycoviruses that infect pathogenic fungi is limited. We performed an extensive patterns 98 characterized RNA from eight phytopathogenic fungi. The GC GC3s contents have a wide variation 29.35% 64.62% 24.32% 97.13%, respectively. Mycoviral CUB weak, natural selection plays major role formation mycoviral pattern. In this study, we...
Previous research has unearthed the integration of coat protein (CP) gene from alphapartitivirus into plant genomes. Nevertheless, prevalence this horizontal transfer (HGT) between partitiviruses and cellular organisms remains an enigma. In our investigation, we discovered a novel partitivirus, designated Sclerotinia sclerotiorum 1 (SsAPV1), hypovirulent strain sclerotiorum. Intriguingly, traced homologs SsAPV1 CP to genomes, including Helianthus annuus. To delve deeper, employed...
Abstract Many plant pathogens secrete effector proteins into the host to suppress immunity and facilitate pathogen colonization. The necrotrophic Sclerotinia sclerotiorum causes severe diseases results in enormous economic losses, which secreted play a crucial role. SsCVNH was previously reported as protein, its expression is significantly upregulated at 3 h after inoculation on plant. Here, we further demonstrated that deletion of leads attenuated virulence. Heterologous Arabidopsis...
Codon usage bias is a universal feature of all genomes. Although codon has been shown to regulate mRNA and protein levels by influencing decay transcription in eukaryotes, little or no genome-wide correlations between are detected mammalian cells, raising doubt on the significance effect gene expression. Here we show that gene-specific regulation reduces correlations: Constitutively expressed genes exhibit much higher than differentially from fungi human cells. Using Drosophila S2 cells as...
Abstract Carbon fixation pathway of plants (CFPP) in photosynthesis converts solar energy to biomass, bio-products and biofuel. Intriguingly, a large number heterotrophic fungi also possess enzymes functionally associated with CFPP, raising the questions about their roles fungal development evolution. Here, we report on presence 17 CFPP (ten Calvin-Benson-Basham reductive pentose phosphate seven C4-dicarboxylic acid cycle) genome Sclerotinia sclerotiorum , phytopathogenic fungus only two...
The seed microbiota is an important component given by nature to plants, protecting seeds from damage other organisms and abiotic stress. However, little known about the dynamic changes potential functions of during development. In this study, we investigated composition rapeseed (Brassica napus). A total 2496 amplicon sequence variants (ASVs) belonging 504 genera in 25 phyla were identified, all sampling stages divided into three groups. flower buds, young pods, at 20 days after flowering...
Mitogen-activated protein kinase (MAPK) cascades play a central role in cellular growth, proliferation, and survival. MAPK cascade genes have been extensively investigated model plants, mammals, yeast, fungi but are not characterized Plasmodiophora brassicae, which causes clubroot disease cruciferous plants. Here, we identified 7 PbMAPK, 3 PbMAPKK, 9 PbMAPKKK the P. brassicae genome. Transcriptional profiling analysis demonstrated that several MAPK, (MAPKK), (MAPKKK) were preferentially...
Strain IBc-114 was isolated from a gray mold lesion and identified as the fungus Schizophyllum commune. In this strain, two mycoviruses, commune RNA virus 1 (ScRV1, C_AA053475.1) Botrytis cinerea mitovirus 9 strain (BcMV9/IBc-114, C_AA053476.1), were characterized. ScRV1 has flexuous filamentous particles about 20 ± 2.1 nm in diameter 1000 94.2 length. The genome of is 7370 nt length contains open reading frames (ORFs) which encode polyprotein coat protein, respectively. 1967 aa, including...
Heredity and variation are inherent characteristics of species mainly reflected in the stability genome; former is relative, while latter continuous. However, whether life has both stable genomes extremely diverse at same time unknown. In this study, we isolated Sclerotinia sclerotiorum strains from sclerotium samples Quincy, Washington State, USA, found that four single-sclerotium-isolation (PB4, PB273, PB615, PB623) had almost identical to reference strain 1980 west Nebraska 40 years ago....
ABSTRACT Under amino acid starvation condition, eukaryotic organisms activate a general control response. In Neurospora crassa , Cross Pathway Control-1 (CPC-1), the ortholog of Saccharomyces cerevisiae bZIP transcription factor GCN4, functions as master regulator Codon usage biases are universal feature genomes and critical for regulation gene expression. Although codon has also been implicated in protein structure function, genetic evidence supporting this conclusion is very limited. Here...