A5044502508- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Aquaculture disease management and microbiota
- Genetic diversity and population structure
- interferon and immune responses
- MicroRNA in disease regulation
- Muscle Physiology and Disorders
- Fish Biology and Ecology Studies
- Cancer-related molecular mechanisms research
- Aquaculture Nutrition and Growth
- Molecular Biology Techniques and Applications
- Circular RNAs in diseases
- RNA modifications and cancer
- RNA regulation and disease
- RNA Research and Splicing
- Histone Deacetylase Inhibitors Research
- Peroxisome Proliferator-Activated Receptors
- Fish biology, ecology, and behavior
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Cardiomyopathy and Myosin Studies
- Amino Acid Enzymes and Metabolism
- Drug Transport and Resistance Mechanisms
- Immune Response and Inflammation
- Animal Genetics and Reproduction
Guizhou University
2016-2023
Changsha University
2012-2018
Hunan Agricultural University
2016
Huazhong Agricultural University
2013-2015
The sinipercids are a group of 12 species freshwater percoid fish endemic to East Asia and their phylogenetic placements have perplexed generations taxonomists. We cloned sequenced the complete mitochondrial DNA (mtDNA) three sinipercid fishes (Siniperca chuatsi, S. kneri, scherzeri) characterize compare genomes. genomes scherzeri were 16,496, 17,002, 16,585 bp in length, respectively. organization is similar those reported from other genomes, which contains 37 genes (13 protein-coding...
Real-time quantitative reverse transcription PCR (RT-qPCR) is one of the most effective and sensitive techniques in gene expression assay, for which selection reference genes a prerequisite. In teleost species, such as Chinese perch, profiling miRNAs RT-qPCR has not been intensively studied. present study, profiles six (miR-101a, miR-146a, miR-22a, miR-23a, miR-26a let-7a) small nuclear RNA (U6) were assayed with different adult tissues, developmental stages growth conditions Siniperca...
In this study, we cloned and sequenced the complete mitochondrial DNAs of Chinese mandarin fish, Siniperca scherzeri populations from four different areas Yangtze River Pearl systems in China. system, fish samples were taken three regions, Dongting Lake (DT), Poyang (PL) Xiangjiang (XR). collected Lijiang (LR). The sizes their genome 16,502 bp (LR), 16,508 (PL), (XR) respectively. organization S. genomes was similar to those reported other genomes. Phylogenetic analyses using N–J...
Sinocyclocheilus furcodorsalis, a typical cavefish, has evolved some striking characters, for example loss of its eyes and reduction in melanin pigmentation, can serve as good model system evolutionary adaptation developmental mechanisms. So we cloned the complete mitochondrial DNA S. furcodorsalis (16,581 bp), which is similar to those reported from other fish genomes, containing 37 genes (13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs) major noncoding control region. The...
Abstract Muscle growth is mainly achieved by two processes, hyperplasia (fiber number increase) and hypertrophy size increase), regulated the myogenic regulatory factor (MRF) family. The muscle fiber composition expressional patterns of four MRFs (Myf5, MRF4, MyoD, myogenin [MyoG]) were characterized in white Ricefield Eels Monopterus albus ranging from 5 to 180 g. Results showed that mean diameters gradually increased as body mass 114 small fibers (diameter < 20 μm) found highest...
Culter dabryi, a medium-sized economic cyprinid fish, is widely distributed in East Asia. We sequenced the complete mitochondrial genome of this species. The 16,622 bp length, and consists 22 transfer RNA genes, 13 protein-coding two ribosomal genes non-coding control region with structural organization conserved relative to that other fishes. overall base composition C. dabryi descending order A 31.51%, C 27.60%, T 24.95% G 15.94%, slight + bias. mitogenome sequence data may provide useful...
In this study, we cloned and sequenced the complete mitochondrial DNA (mtDNA) of Synechogobius hasta to characterize compare their genomes. The total length genome is 16,655 bp with an accession number KM891736. organization genomes was similar those reported from other fish containing 37 genes (13 protein-coding genes, 2 ribosomal RNA 22 transfer RNAs) a major non-coding control region. Except for ND6 8 tRNAs, all are encoded on heavy strand. base composition 13 in third position relatively...
In this study, we cloned and sequenced the complete mitochondrial DNA of ricefield eel populations from four different areas (Guizhou province, Guangxi Hunan Guangdong province) in China. The genome was 16,622 bp length deposited GenBank with accession numbers KP779622-KP779625. organizations mitogenomes were similar to those other teleost species which contained 13 protein-coding genes, two rRNA 22 tRNA as well a putative control region (CR). Most these genes encoded on H-strand, except for...
In this study, we determined the complete mitochondrial DNA sequence of hybrid Ctenopharyngodon idellus (♀) × Elopichthys bambusa (♂). The total length genome was 16,609 bp (accession number KM401549), with base composition 31.89% A, 26.33% C, 15.68% G, 26.11% T. contained 2 ribosomal RNA genes, 13 protein-coding 22 transfer and a major non-coding control region (D-loop region). Except for ND6 8 tRNAs, all other genes were encoded on heavy strand. arrangements these similar to that found in...
The extant freshwater sinipercids represent a group of 12 species and they are endemic to East Asia. In this study, we cloned sequenced the complete mitochondrial DNA Siniperca obscura from Lijiang River. size genome is 16,492 bp. organization contained 37 genes (13 protein-coding genes, 2 ribosomal RNA 22 transfer RNAs) major non-coding control region as well those reported sinipercid fishes. Among 13 three reading-frame overlaps were found: ATP8 ATP6 overlap by 10 nucleotides ND4 ND4L 7...
Objective: The essential myosin light chains (EMLC) are the major structural and regulatory proteins that control actin-myosin interaction in striated muscles. purpose of this work is to provide a better understanding about EMLC properties their expression pattern during muscle development mandarin fish. Material Methods: cDNA library was constructed isolate characterize MLC sequences. nucleotide sequences inferred amino acid were analyzed. Whole-mount situ hybridization carried out...
Protein kinase RNA-activated (PKR) is an important component of the innate immune system induced by interferon. This antiviral protein senses a variety stresses and pathogen-driven double-stranded RNAs (dsRNAs). In present study, we cloned characterized PKR gene from Dabry's sturgeon (Acipenser dabryanus). Bioinformatics analysis revealed that AdPKR comprises coding sequence (CDS) 1848 bp, encoding 616 amino acids (aa) polypeptide. shares high aa identity/similarity with proteins other fish,...
Abstract The myocyte‐specific enhancer factor 2 (MEF2) gene family members are well‐established regulators in the control of muscle repair, regeneration, and development. In this study, we cloned CDS sequences four MEF2 ricefield eel, their coding (CDS) lengths were 1449 bp, 1419 1431 1608 respectively. Sequence analysis demonstrated that three them shared conserved protein domains, including a MADS‐Box, inside SRF‐TF adjacent HJURP‐C while MEF2B have no domains. Phylogenetic tree analyses...
We cloned and sequenced the complete mitochondrial DNA (mtDNA) of Cirrhinus mrigala (♀) × Labeo rohita (♂) by PCR-based method. The total mtDNA was 16,595 bp. It consisted 13 protein-coding genes, 22 tRNAs, 2 rRNA one putative control region (D-loop region). overall base composition on strand as follows A: 32.09%, G: 15.51%, C: 27.95%, T: 24.45%, A + T content 56.54%. All genes initiated typical ATG codon, ten ended with stop codon TAA or TAG, while COII2, ND4 Cytb terminated GCC, TAT GCT....
In this study, the complete mitochondrianl genome of Procypris merus was sequenced. It determined to be 16,581 bp and included 13 protein-coding genes, 22 tRNA 2 ribosomal RNA genes 1 non-coding region (D-loop). The descending order base composition on heavy strand 31.91% A, 24.95% T, 27.39% C, 15.75% G, which is similar other cyprinid fish mitochondrial genomes. All had ATG as start codon but stop codons have three types. Night end with TAA or TAG, COII, ND4, ND6 Cytb terminate an...