A5044063771- Microtubule and mitosis dynamics
- Biofuel production and bioconversion
- Polysaccharides and Plant Cell Walls
- Bacteriophages and microbial interactions
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Polysaccharides Composition and Applications
- Plant Molecular Biology Research
- Advanced Cellulose Research Studies
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Cellular Mechanics and Interactions
- 14-3-3 protein interactions
- Advancements in Transdermal Drug Delivery
- Legume Nitrogen Fixing Symbiosis
- Enterobacteriaceae and Cronobacter Research
- DNA Repair Mechanisms
- Dermatology and Skin Diseases
- Mitochondrial Function and Pathology
- Advanced Fluorescence Microscopy Techniques
- Wound Healing and Treatments
- Micro and Nano Robotics
- Microbial Metabolic Engineering and Bioproduction
- Protist diversity and phylogeny
- RNA Research and Splicing
Pennsylvania State University
2018-2024
The University of Texas Southwestern Medical Center
2023
Binghamton University
2016-2018
National Renewable Energy Laboratory
2009
San Diego Supercomputer Center
2007
Merck & Co., Inc., Rahway, NJ, USA (United States)
1987-1993
Consejo Nacional de Investigaciones Científicas y Técnicas
1991
University of California, San Diego
1981-1983
University of California, Los Angeles
1981
The chromosomal replication origin (oriC) of Vibrio harveyi has been isolated on a plasmid and shown to function as an in Escherichia coli. nucleotide sequence the V. oriC was determined. From comparison this with sequences five enteric bacteria, we derived consensus bacterial origins that E. This identifies 122 positions within where substitutions can occur without loss function. These are clustered rather than scattered. Four interrelated nine-base-pair repeats eight dam methylation...
Xanthomonas campestris produces copious amounts of a complex exopolysaccharide, xanthan gum. Nonmucoid mutants, defective in synthesis polysaccharide, were isolated after nitrosoguanidine mutagenesis. To isolate genes essential for polysaccharide (xps), genomic library X. DNA, partially digested with SalI and ligated into the broad-host-range cloning vector pRK293, was constructed Escherichia coli. The pooled clone bank conjugated en masse from E. coli three nonmucoid mutants by using...
Cellobiohydrolases are the most effective single component of fungal cellulase systems; however, their molecular mode action on cellulose is not well understood. These enzymes act to detach and hydrolyze cellodextrin chains from crystalline in a processive manner, carbohydrate-binding module (CBM) thought play an important role this process. Understanding interactions between CBM at level can assist greatly formulating selective mutagenesis experiments confirm function CBM. Computational...
Microtubule polymerization dynamics result from the biochemical interactions of αβ-tubulin with polymer end, but a quantitative understanding has been challenging to establish. We used interference reflection microscopy make improved measurements microtubule growth rates and fluctuations in presence absence GTP hydrolysis. In hydrolysis, microtubules grew steadily very low fluctuations. These data were best described by computational model implementing slow assembly kinetics, such that rate...
The replication origins of viral and complementary strands bacteriophage M13 DNA are contained within a 507-nucleotide intergenic region the genome. Chimeric plasmids have been constructed by inserting restriction endonuclease fragments into plasmid pBR322. Replication these hybrid plasmids, under conditions not permissive for replicon, depends on specific segments origin presence helper virus. Thus M13-infected polA- Escherichia coli can be transformed to ampicillin resistance that...
Genes required for xanthan polysaccharide synthesis (xps) are clustered in a DNA region of 13.5 kb the chromosome Xanthomonas campestris. Plasmid pCHC3 containing 12.4-kb insert xps genes has been suggested to include gene involved pyruvylation gum (N.E. Harding, J.M. Cleary, D.K. Cabañas, I. G. Rosen, and K. S. Kang, J. Bacteriol. 169:2854-2861, 1987). An essential step toward understanding biosynthesis enable genetic manipulation structure is determination biochemical function encoded by...
A genetic and biochemical analysis of Xanthomonas campestris chromosomal functions required for xanthan polysaccharide synthesis (xps) was undertaken. Seven xps DNA regions were isolated after conjugation chemically induced non-mucoid mutants with a genomic library X. DNA. No overlapping segments between detected, based on physical mapping, indicating the unlinked character these regions. Clones complementing several different belonging to same region contained Complementation analysis,...
A cloned 270-nucleotide fragment from the origin region of M13 duplex replicative form DNA confers an M13-dependent replication mechanism upon plasmid vector pBR322. This insert permits helper-dependent hybrid in polA cells which are unable to replicate pBR322 replicon alone. Using vitro techniques, we have constructed several plasmids containing deletions DNa insert. The endpoints these been determined by sequence analysis and correlated with transformation properties each plasmid....
The chromosomal DNA replication origins (oriC) from two members of the family Enterobacteriaceae, Enterobacter aerogenes and Klebsiella pneumoniae, have been isolated as functional in Escherichia coli. SalI restriction fragments 17.5 10.2 kilobase pairs, cloned E. K. respectively, were found to be between asnA uncB genes, are coli Salmonella typhimurium chromosomes. Plasmids containing oriC E aerogenes, S. replicate cell-free enzyme system (Fuller, et al., Proc. Natl. Acad. Sci. U.S.A....
Contemporary studies have revealed dramatic changes in the diversity of bacterial microbiota between healthy and diseased skin. However, prevailing use swabs to extract microorganisms has meant that only population ‘snapshots’ are obtained, all spatially resolved information growth is lost. Here we report on temporospatial Staphylococcus aureus surface human stratum corneum (SC); outermost layer This species dominates populations skin with atopic dermatitis (AD). We first establish...
The nucleotide sequences of the Enterobacter aerogenes and Klebsiella pneumoniae DNA replication origins (oriC) were determined compared with those Escherichia coli Salmonella typhimurium. Four interrelated, 9-base-pair repeats identified from conserved regions within minimal origin. Evolutionary rates calculated origin yielded a quantitative phylogenic tree which agreed taxonomic classification these genera.
GTP-tubulin is preferentially incorporated at growing microtubule ends, but the biochemical mechanism by which bound nucleotide regulates strength of tubulin:tubulin interactions debated. The 'self-acting' (cis) model posits that (GTP or GDP) to a particular tubulin dictates how strongly interacts, whereas 'interface-acting' (trans) interface two dimers determinant. We identified testable difference between these mechanisms using mixed simulations elongation: with self-acting nucleotide,...
Axons and dendrites are distinguished by microtubule polarity. In Drosophila, dominated minus-end-out microtubules, whereas axons contain plus-end-out microtubules. Local nucleation in generates microtubules both orientations. To understand why dendritic does not disrupt polarity, we used live imaging to analyze the fate of generated at branch points. We found that they had different rates success exiting based on orientation: correctly oriented succeeded leaving about twice as often...
GTP-tubulin is preferentially incorporated at growing microtubule ends, but the biochemical mechanism by which bound nucleotide regulates strength of tubulin:tubulin interactions debated. The ‘self-acting’ (cis) model posits that (GTP or GDP) to a particular tubulin dictates how strongly interacts, whereas ‘interface-acting’ (trans) interface two dimers determinant. We identified testable difference between these mechanisms using mixed simulations elongation: with self-acting nucleotide,...
Article abstract–Since October 1991, nearly 51,000 Cubans have been afflicted in an outbreak of optic and peripheral neuropathies. To begin investigation the possible role mitochondrial DNA (mtDNA) mutations outbreak, we studied mtDNA from 14 affected two unaffected for 12 associated with Leber9s hereditary neuropathy. Eleven probands (12 patients) had neuropathy only. We also relatives one proband. identified mutations, at nucleotides 11778 3460, 11 Although this data set is too small to...