A5080125001- Microfluidic and Bio-sensing Technologies
- Electrowetting and Microfluidic Technologies
- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Virology and Viral Diseases
- IoT and Edge/Fog Computing
- Single-cell and spatial transcriptomics
- Intravenous Infusion Technology and Safety
University of Toronto
2015-2022
Toronto Public Health
2015-2020
Portable digital microfluidic serological immunoassays for measles and rubella were developed evaluated in a remote setting.
Abstract We introduce Digital microfluidic Isolation of Single Cells for -Omics (DISCO), a platform that allows users to select particular cells interest from limited initial sample size and connects single-cell sequencing data their immunofluorescence-based phenotypes. Specifically, DISCO combines digital microfluidics, laser cell lysis, artificial intelligence-driven image processing collect the contents single heterogeneous populations, followed by analysis genomes transcriptomes...
Nanostructured microelectrodes (NMEs) are three-dimensional electrodes that have superb sensitivity for electroanalysis. Here we report the integration of NMEs with versatile fluid-handling system digital microfluidics (DMF), eventual application to distributed diagnostics outside laboratory. In new methods reported here, indium tin oxide DMF top plates were modified include Au as well counter and pseudoreference electrodes. The was observed outperform planar sensing type typically...
Finger-stick blood sampling is convenient for point of care diagnostics, but whole samples are problematic many assays because severe matrix effects associated with cells and cell debris. We introduce a new digital microfluidic (DMF) diagnostic platform integrated porous membranes blood-plasma separation from finger-stick volumes, capable performing complex, multi-step, assays. Importantly, the can be directly loaded onto device by finger "dab" user-friendly operation. characterize...
A digital microfluidic system was developed for portable Zika virus diagnosis, featuring integrated, automated sample extraction and amplification.
Microfluidics has become an important tool that is useful for a wide range of applications. A drawback microfluidics many the techniques are commonly used to fabricate devices not widely accessible, scalable high‐volume manufacturing processes, or both. Recently, number printing strategies were originally developed other applications have been applied microfluidic device fabrication. These techniques, which include inkjet (IJP), screen (SP), and solid wax (SWP), proposed transformative...
Abstract Background Blood typing, donor compatibility testing, and hematocrit analysis are common tests that important in many clinical applications, including those found high-stakes settings such as the trauma center. These typically performed centralized laboratories with sample batching; minutes lost this mode can lead to adverse outcomes, especially for critical-care patients. As a step toward providing rapid results at bedside, we developed point-of-care hemagglutination system relying...
The Democratic Republic of the Congo (DRC) has a high measles incidence despite elimination efforts and yet to introduce rubella vaccine. We evaluated performance prototype rapid digital microfluidics powered (DMF) enzyme-linked immunoassay (ELISA) assessing infection, by testing for immunoglobulin M (IgM), immunity from natural infection or vaccine, G (IgG), in outbreak settings. Field evaluations were conducted during September 2017, Kinshasa province, DRC. Blood specimens collected an...