Decellularized extracellular matrices (dECMs) have shown enormous potential for the biofabrication of tissues due to their biomimetic properties that promote enhanced cellular interaction and tissue regeneration. However, schemes requiring electrostimulation pose an additional constraint insulating natural materials. Here, we propose a methacryloyl-modified decellularized small intestine submucosa (SISMA) hydrogel, embedded with graphene oxide (GO) nanosheets, extrusion-based 3D bioprinting...

Antibiotic resistance has become a major public health problem generated by their excessive and inappropriate use. This is worrisome because multiple microbial infections that could traditionally be treated without complications are now considerably challenging to treat. In this regard, research in field been focused on searching for new molecules capable of arresting these with high effectiveness, including antimicrobial peptides (AMP) various nanomaterials. Here, we proposed novel topical...

Antibiotic resistance is a worldwide public health problem due to the costs and mortality rates it generates. However, large pharmaceutical industries have stopped searching for new antibiotics because of their low profitability, given rapid replacement imposed by increasingly observed acquired microorganisms. Alternatively, antimicrobial peptides (AMPs) emerged as potent molecules with much lower rate generation. The discovery these carried out through extensive in vitro screenings either...

Therapeutic drugs for Alzheimer's disease have been extensively studied due to its recurrence and abundance among neurodegenerative diseases. It is thought that the accumulation of amyloid precursor protein (APP) products, a consequence an up-regulation β-site APP-cleaving enzyme 1 (BACE1), main triggering mechanism during early stages disease. This study aims explore ability multifunctional conjugate based on magnetite nanoparticles cellular delivery siRNA against expression

Over the past decade, gene therapies have attracted much attention for development of treatments various conditions, including cancer, neurodegenerative diseases, protein deficiencies, and autoimmune disorders. Despite benefits this approach, several challenges are yet to be solved reach clinical implementation. Some these include low transfection rates, limited stability under physiological specificity towards target cells. An avenue overcome such issues is deliver with aid potent...

Nutraceutical formulations based on probiotic microorganisms have gained significant attention over the past decade due to their beneficial properties human health. Yeasts offer some advantages other organisms, such as immunomodulatory properties, anticancer effects and effective suppression of pathogens. However, one main challenges for oral administration is ensuring that cell viability remains high enough a sustained therapeutic effect while avoiding possible substrate inhibition issues...

As life expectancy continues to increase, the inevitable weakening and rupture of bone tissue have grown as concerns in medical community, thus leading need for adhesive materials suitable repair applications. However, current commercially available adhesives face certain drawbacks that prevent proper repair, such low biocompatibility, poor adhesion wet surfaces, high polymerization temperatures. This work aims develop an injectable photo-responsive chitosan methacrylate/graphene oxide...

One of the major challenges modern pharmacology is development systems for delivery therapeutic molecules in a controlled and localized manner. strategy to use nanostructured supports, which are well suited carry large number on per mass basis. A challenge these supports is, however, their limited ability bypass cell membrane. Recent studies propose that overcome this issue, potent translocating cell-penetrating peptides (CPPs) can be conjugated surfaces.Here, we antimicrobial CPP buforin II...

Outer membrane protein A (OmpA) has been extensively studied in Gram-negative bacteria due to its relevance the adhesion of pathogens host cells and surfactant capabilities. It consists a hydrophobic β-barrel domain hydrophilic periplasmic domain, that confers OmpA an amphiphilic structure. This study aims elucidate capacity Escherichia coli translocate liposomal membranes serve as potential cell-penetrating vehicle. We immobilized on magnetite nanoparticles investigated possible functional...

Magnetite nanoparticles (MNPs) have gained significant attention in several applications for drug delivery. However, there are some issues related to cell penetration, especially the transport of cargoes that show limited membrane passing. A widely studied strategy overcome this problem is encapsulation MNPs into liposomes form magnetoliposomes (MLPs), which capable fusing with membranes achieve high delivery rates. This study presents a low-cost microfluidic approach synthesis and...

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease. Therefore, development of novel technologies and strategies to treat PD a global health priority. Current treatments include administration Levodopa, monoamine oxidase inhibitors, catechol-O-methyltransferase anticholinergic drugs. However, effective release these molecules, due limited bioavailability, major challenge for treatment PD. As strategy solve this challenge, in study we...

Non-centrifugal cane sugar (NCS) is a traditional sweetener in most sugarcane regions of the world. In Colombia, this product has socio-economic importance due to extensive cultivation area and high consumption rate per capita. NCS processing involves consecutive stages thermal that begin with juice extraction, clarification, evaporation, finish syrup crystallization into solid commercial product, identified as NCS. Sugarcane known have natural content polyphenols, amino acids, vitamins,...

Abstract Mucopolysaccharidosis IV A (MPS IVA) is a lysosomal disorder caused by mutations in the GALNS gene. Consequently, glycosaminoglycans (GAGs) keratan sulfate and chondroitin 6-sulfate accumulate lumen. Although enzyme replacement therapy has shown essential advantages for patients, several challenges remain to overcome, such as limited impact on bone lesion recovery of oxidative profile. Recently, we validated CRISPR/nCas9-based gene with promising results an vitro MPS IVA model. In...

Melanoma is an aggressive type of skin cancer that accounts for over 75% deaths despite comprising less than 5% all cancers. Despite promising improvements in surgical approaches melanoma resection, the survival undetectable microtumor residues has remained a concern. As result, hyperthermia- and drug-based therapies have grown as attractive techniques to target treat cancer. In this work, we aim develop stimuli-responsive hydrogel based on chitosan methacrylate (ChiMA), porcine small...

Gene delivery has emerged as a promising alternative to conventional treatment approaches, allowing for the manipulation of gene expression through insertion, deletion, or alteration. However, susceptibility components degradation and challenges associated with cell penetration necessitate use vehicles effective functional delivery. Nanostructured vehicles, such iron oxide nanoparticles (IONs) including magnetite (MNPs), have demonstrated significant potential applications due their chemical...

Over the past decades, Colombia has suffered complex social problems related to illicit crops, including forced displacement, violence, and environmental damage, among other consequences for vulnerable populations. Considerable effort been made in regulation of predominantly Cannabis sativa, leading advances such as legalization medical cannabis its derivatives, improvement leaving an open window development scientific knowledge explore alternative uses. It is estimated that C. sativa can...

Nanomedicine is entering a high maturity stage and ready to reach full translation into the clinical practice. This because of ample spectrum applications enabled by large arsenal nanostructured materials. In particular, bimetallic patchy core/shell nanoparticles offer tunable surfaces that allow multifunctional responses. Despite their attractiveness, major challenges regarding environmental impact biocompatibility obtained materials are yet be solved. Here, we developed green synthesis...

The gangliosidoses GM2 are a group of pathologies mainly affecting the central nervous system due to impaired ganglioside degradation inside lysosome. Under physiological conditions, is catabolized by β-hexosaminidase A in activator protein-dependent mechanism. In contrast, uncharged substrates such as globosides and some glycosaminoglycans can be hydrolyzed B. Monogenic mutations on HEXA, HEXB, or GM2A genes arise Tay-Sachs (TSD), Sandhoff (SD), AB variant diseases, respectively. this work,...

Cell-penetrating agents based on functionalized nanoplatforms have emerged as a promising approach for developing more efficient and multifunctional delivery vehicles treating various complex diseases that require reaching different intracellular compartments. Our previous work has shown achieving full cellular coverage high endosomal escape rates is possible by interfacing magnetite nanoparticles with potent translocating peptides such Buforin II (BUF-II). In this work, we extended an to...

The limited delivery of cargoes at the cellular level is a significant challenge for therapeutic strategies due to presence numerous biological barriers. By immobilizing Buforin II (BUF-II) peptide and OmpA protein on magnetite nanoparticles, new family cell-penetrating nanobioconjugates was developed in previous study. We propose this study extend strategy silica nanoparticles (SNPs) silanized fullerenol (F) as nanostructured supports conjugating these potent agents. same molecule...

At the beginning of 2020, pandemic caused by SARS-CoV-2 virus led to fast sequencing its genome facilitate molecular engineering strategies control pathogen's spread. The spike (S) glycoprotein has been identified as leading therapeutic agent due role in localizing ACE2 receptor host's pulmonary cell membrane, binding, and eventually infecting cells. Due difficulty delivering bioactive molecules intracellular space, we hypothesized that S protein could serve a source membrane translocating...