A5023288754- Nanopore and Nanochannel Transport Studies
- Lipid Membrane Structure and Behavior
- Antimicrobial Peptides and Activities
- Microfluidic and Capillary Electrophoresis Applications
- Bacteriophages and microbial interactions
- Microfluidic and Bio-sensing Technologies
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Membrane Separation Technologies
- Advancements in Transdermal Drug Delivery
- Fuel Cells and Related Materials
- Ion-surface interactions and analysis
- Connective tissue disorders research
- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
Adolphe Merkle Institute
2017-2021
University of Fribourg
2017-2021
Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement
2012-2018
Centre National de la Recherche Scientifique
2012-2013
Université d'Évry Val-d'Essonne
2012-2013
This paper demonstrates that high-bandwidth current recordings in combination with low-noise silicon nitride nanopores make it possible to determine the molecular volume, approximate shape, and dipole moment of single native proteins solution without need for labeling, tethering, or other chemical modifications these proteins. The analysis is based on modulations caused by translation rotation through a uniform electric field inside nanopore. We applied this technique nine show measured...
Glycosaminoglycans are biologically active anionic carbohydrates that among the most challenging biopolymers with regards to their structural analysis and functional assessment. The potential of newly introduced biosensors using protein nanopores have been mainly described for nucleic acids date, has here applied this polysaccharide-based third class bioactive biopolymer. This nanopore approach harnessed in study analyze hyaluronic acid glycosamiglycan its depolymerization-derived...
Protein pores recently enabled a breakthrough in bioanalytics by making it possible to sequence individual DNA and RNA strands during their translocation through the lumen of pore. Despite this success overall promise nanopore-based single-molecule analytics, protein have not yet reached full potential for analysis characterization globular biomolecules such as natively folded proteins. One reason is that diameters available are too small accommodating most proteins lumen. The work presented...
MelP5 is a 26 amino acid peptide derived from melittin, the main active constituent of bee venom, with five replacements. The pore-forming activity in lipid membranes attracting attention because forms larger pores and induces dye leakage through liposome at lower concentration than melittin. Studies have so far focused on ensemble measurements membrane impedance; here we extend this characterization an electrophysiological comparison between melittin using planar bilayer recordings. These...
Pore-forming antimicrobial peptides (AMPs) are attracting interest as cytolytic antibiotics and drug delivery agents with potential use for targeting cancer cells or multidrug-resistant pathogens. Ceratotoxin A (CtxA) is an insect-derived AMP 36 amino acids that thought to protect the eggs of medfly Ceratitis capitata against Single channel recordings using planar lipid bilayers have shown CtxA forms pores well-defined conductance states resembling those alamethicin; it also one largest...
Abstract: Pore-forming peptides are of interest due to their antimicrobial activity and ability form gateways through lipid membranes. Chemical modification these makes it possible arrange several peptide monomers into well-defined pore-forming structures using various templating strategies. These templated super-structures can exert at significantly lower total concentration than untemplated equivalents. In addition, the chemical moieties used for may be functionalized interact specifically...