This is a wide epidemiological study of 499 E. coli isolates recovered from 179 outbreaks enteric colibacillosis pig production farms in Spain during period 10 years. Most samples were diarrheagenic cases occurred the post wean (PWD) which showed to be significantly associated with ETEC (67%) followed by aEPEC (21.7%). On contrary, was more prevalent (60.3%) among diarrheas suckling piglets, (38.8%). STEC/ETEC or STEC 11.3% and 0.9% PWD neonatal diarrhea, respectively. Detection F4...

Escherichia coli is the main pathogen responsible for extraintestinal infections. A total of 196 clinical E. consecutively isolated during 2016 in Spain (100 from Lucus Augusti hospital Lugo) and France (96 Beaujon Clichy) were characterized. Phylogroups, clonotypes, sequence types (STs), O:H serotypes, virulence factor (VF)-encoding genes antibiotic resistance determined. Approximately 10% infections caused by ST131 isolates both hospitals approximately 60% these belonging to only 10 STs...

Antimicrobial agents are crucial for the treatment of many bacterial diseases in pigs, however, massive use critically important antibiotics such as colistin, fluoroquinolones and 3rd-4th-generation cephalosporins often selects co-resistance. Based on a comprehensive characterization 35 colistin-resistant Escherichia coli from swine enteric colibacillosis, belonging to prevalent Spanish lineages, aims present study were investigate characteristics E. clones successfully spread assess...

The aim of the present study was to examine prevalence and determine molecular characteristics extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) causing bacteraemia in a Spanish Hospital over 12-year period (2000 2011). As far as we know, this is first which has investigated compared serotypes, phylogroups, clonotypes, virotypes, PFGE profiles ST131 non-ST131 clones ESBL-EC isolates. Of 2,427 E. bloodstream isolates, 96 (4.0%) were positive for ESBL production: 40 CTX-M-15,...

The present study was carried out to evaluate the prevalence of sequence type 131 (ST131) among 188 extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) collected in 2015 Lucus Augusti University hospital (Lugo, Spain) and AP-HP Beaujon (Clichy, France) with regard other STs characterize, types ESBL produced, serotypes, virulence factor (VF)-encoding genes ST131 clades subclades. detected 33 (39.1%) 46 (47.9%) isolates Beaujon, respectively. 109 remaining displayed 57 STs,...

Porcine Escherichia coli ST131 isolates are scarcely documented. Here, whole genome sequencing, core (CG) and plasmidome analysis of seven collected from diarrhoeic piglets four pork meat was performed. All the 11 belonged to serotype O25b:H4 clade B, showed fimH22 allele or mutational derivatives. The porcine possessed virulence traits that classified as avian pathogenic, uropathogenic extraintestinal pathogenic E. like status (APEC, UPEC ExPEC like) constituted virotype D. CG performed for...

Under a one health perspective and the worldwide antimicrobial resistance concern, we investigated extraintestinal pathogenic Escherichia coli (ExPEC), uropathogenic E. (UPEC), multidrug resistant (MDR) from 197 isolates recovered healthy dogs in Spain between 2013 2017. A total of 91 (46.2%) were molecularly classified as ExPEC and/or UPEC, including 50 clones, among which (i) four clones dominant (B2-CH14-180-ST127, B2-CH52-14-ST141, B2-CH103-9-ST372 F-CH4-58-ST648) (ii) 15 had been...

The prevalence of Shiga toxin (Stx)-producing Escherichia coli (STEC) was determined by evaluating its presence in faecal samples from 155 heifers, and 254 dairy cows 21 farms at North Portugal sampled between December 2017 June 2019. STEC heifers (45%) significantly higher than lactating (16%) ( p <0.05, Fisher exact test statistic value is <0.00001). A total 133 were isolated, 24 (13.8%) carried Shiga-toxin 1 stx1 ) genes, 69 (39.7%) 2 stx2 40 (23%) both . Intimin eae virulence gene...

Escherichia coli biofilm formation has mostly been assessed in specific pathogenic E. groups. Here, we the early (EBF), i.e., adhesion stage, using BioFilm Ring Test® on 394 clinical isolates (EC) [196 consecutively isolated (CEC) 2016 and 198 ESBL-producing (ESBLEC) 2015]. Then, biofilm-forming ability was contrasted with phylogroups, clonotypes (fumC-fimH), sequence types (STs), all being used to define clones, virulence factors (VF), FimB.According both production levels at 2, 3, 5 h, EBF...

Escherichia coli O25b:H4 sequence type 131 (ST131), which is resistant to fluoroquinolones and a producer of CTX-M-15, globally one the major extraintestinal pathogenic E. (ExPEC) lineages. Phylogenetic analyses showed that multidrug-resistant ST131 strains belong clade C, recently emerged from B by stepwise evolution. It has been hypothesized features other than multidrug resistance could contribute this dissemination since global ExPEC lineages (ST73 ST95) are mostly antibiotic...

Escherichia coli is the main pathogen responsible for extraintestinal infections. A total of 196 clinical E. consecutively isolated during 2016 in Spain (100 from Lucus Augusti hospital Lugo) and France (96 Beaujon Clichy) were characterized. Phylogroups, clonotypes, sequence types (STs), O:H serotypes, virulence factor (VF)-encoding genes antibiotic resistance determined. Approximately 10% infections caused by ST131 isolates both hospitals approximately 60% these belonging to only 10 STs...

Abstract Self-synthesizing transposons are integrative mobile genetic elements (MGEs) that encode their own B-family DNA polymerase (PolB). Discovered a few years ago, they proposed as key players in the evolution of several groups viruses and virus–host interaction machinery. Pipolins most recent addition to group, integrated genomes bacteria from diverse phyla also present circular plasmids mitochondria. Remarkably, pipolins-encoded PolBs proficient polymerases endowed with priming...

Under one-health perspective and the worldwide antimicrobial resistance concern, we investigate extraintestinal pathogenic Escherichia coli (ExPEC), uropathogenic E. (UPEC), multidrug resistant (MDR) from 197 isolates recovered healthy dogs in Spain between 2013 2017. Ninety-one (46.2%) were classified as ExPEC and/or UPEC including 50 clones, among which (i) four clones dominant (B2-CH14-180-ST127, B2-CH52-14-ST141, B2-CH103-9-ST372 F-CH4-58-ST64815) (ii) 15 had been shown to be displayed...

Abstract The role of wildlife in the epidemiology antimicrobial resistance is unclear. Raccoons North America can carry a variety enteric bacteria, with associated resistance, that could infect humans and livestock. potential for raccoons to these bacteria Europe, where they are an invasive species, has not been explored. Our objectives were determine prevalence Escherichia coli from Madrid region Spain whether carriers human pathogens, including verotoxin‐producing E. (VTEC)...

Abstract Escherichia coli of sequence type (ST) 131 resistant to fluoroquinolones and producer CTX-M-15 is globally one the major extraintestinal pathogenic E. (ExPEC). ST131 phylogenesis showed that multidrug-resistant strains belong a clade called C, descending from an ancestral B, comprising mostly antibiotic-susceptible strains. Antibiotic resistance could appear as keys C global success. We hypothesized other features contribute this success since ExPEC clones (ST73, ST95) are...

Abstract Self-synthesizing transposons are integrative mobile genetic elements (MGEs) that encode their own B-family DNA polymerase (PolB). Discovered a few years ago, they proposed as key players in the evolution of several groups viruses and virus-host interaction machinery. Pipolins most recent addition to group, integrated genomes bacteria from diverse phyla also present circular plasmids mitochondria. Remarkably, pipolins-encoded PolBs proficient polymerases endowed with priming...